Dual effect of 2-methoxyestradiol on cell cycle events in human osteosarcoma 143B cells

• Autorzy: Golebiewska J. , Rozwadowski P. , Spodnik J.H. , Knap N. , Wakabayashi T. , Wozniak M.
• Języki publikacji: EN

Abstrakty

EN

We have demonstrated for the first time that the steroid metabolite, 2-methoxy- estradiol (2-ME) is a powerful growth inhibitor of human osteosarcoma 143 B cell line by pleiotropic mechanisms involving cell cycle arrest at two different points and apoptosis. The ability of 2-ME to inhibit cell cycle at the respective points has been found con­centration dependent. 1 uM 2-ME inhibited cell cycle at G1 phase while 10 uM. 2-ME caused G2/M cell cycle arrest. As a natural estrogen metabolite 2-ME is expected to perturb the stability of microtubules (MT) in vivo analogously to Taxol — the MT bind­ing anticancer agent. Contrary to 2-ME, Taxol induced accumulation of osteosarcoma cells in G2/M phase of cell cycle only. The presented data strongly suggest two differ­ent mechanisms of cytotoxic action of 2-ME at the level of a single cell.

Słowa kluczowe

EN

143B cell; 2-methoxyestradiol; apoptosis; cell cycle; man; methoxyestradiol; osteogenic sarcoma zob.osteosarcoma; osteosarcoma

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2002
• Tom: 49
• Numer: 1
• Opis fizyczny: p.59-65,fig.

Twórcy

autor

Golebiewska J.

• Medical University of Gdansk, Debinki 1, 80-211 Gdansk, Poland

autor

Rozwadowski P.

autor

Spodnik J.H.

autor

Knap N.

autor

Wakabayashi T.

autor

Wozniak M.

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