Fenotyp i genotyp szczepow z rodzaju Clostridium wytwarzajacych neurotoksyny botulinowe

• Autorzy: Augustynowicz E. , Gzyl A. , Gniadek G. , Slusarczyk J.
• Języki publikacji: PL

Abstrakty

PL

W pracy podjęto określenie fenotypu i genotypu szczepów C. botulinum. Poszukiwano również genów kodiyących BoNT typu А, В oraz E wśród szczepów rodzaju Clostridium. Uzyskane wyniki wskazują na powszechność występowania genu kodującego BoNT/E wśród szczepów rodząju Clostridium. Analiza fenotypu i genotypu szczepów C. botulinum wykazała występowanie szczepów fenotypu В o genotypie be jak również szczepów fenotypu A o genotypie ae.

EN

Neurotoxins produced by strains of Clostridium sp. are belonging to the most toxic biological substances. In the study phenotypes and genotypes of C. botulinum strains in animal studies in Vivo and on the DNA level were evaluated, respectively. Additionally, the presence of genes encoding BoNT toxins of A, B, and E types among strains of Clostridium sp. were identified. In case of C. botulinum DNA was isolated from vegetative bacterial cells and from spores. Two different genes encoding two different neurotoxins harboured by three strains of Ae biotype/ae genotype, and by two strains of B biotype/be genotype were detected. Additionally, above E type C. botulinum strains, the presence of gene encoding E type neurotoxin, was found in genome of two C. baratii, two C. butyricum, and C. bifidobacterium, and C. oedematicum strains. C. bifidobacterium and C. oedematicum strains positive for presence of gene encoding E type neurotoxin, were found negative for E neurotoxin production in vivo in TN test. The study indicates that genes encoding BoNT/E neurotoxins are very common among Clostridium species. Phenotype and genotype analysies indicated co-presence of В phenotype together with be genotype and A phenotype together with ae genotype among C. botulinum strains.

Słowa kluczowe

PL

neurotoksyny botulinowe; genotyp; szczepy bakteryjne; neurotoksyny; fenotyp; bakterie; Clostridium; toksycznosc

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2003
• Tom: 55
• Numer: 3
• Opis fizyczny: s.244-252,tab.,bibliogr.

Twórcy

autor

Augustynowicz E.

• Panstwowy Zaklad Higieny, Warszawa

autor

Gzyl A.

autor

Gniadek G.

autor

Slusarczyk J.

Bibliografia

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