The activity of lipoxygenase in Arabidopsis thaliana [L.] Heynh - A preliminary study

• Autorzy: Skorzynska-Polit E , Krupa Z.
• Języki publikacji: EN

Abstrakty

EN

The activity of lipoxygenase (EC 1.13.11.12) in Arabidopsis thaliana (L.) Heynh seedlings and mature plants was estimated spectrophotometrically at 234 nm. Linoleic acid was used as a substrate. Lipoxygenase activity showed two pH optima: at 7.0 and 10.0 in seedlings, and at pH 8.0 and 10.0 in leaves of mature plants. Seven-week-old plants were transferred to a hydroponic system and treated with different concentrations of Cd2+ or Cu2+ [in μM]: 0, 5, 25, 50, 100 for 7 days. The lipoxygenase activities at pH 8.0 and 10.0 depended on the metal that was added to the nutrient solution. The main change in lipoxygenase activity was under Cd2+ stress at pH 8.0 and under Cu2+ excess at pH 10.0.

Słowa kluczowe

EN

linoleic acid; lipoxygenase; Arabidopsis thaliana; cadmium; copper; fatty acid; plant

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2003
• Tom: 08
• Numer: 2
• Opis fizyczny: p.279-284,fig.

Twórcy

autor

Skorzynska-Polit E

• Maria Sklodowska-Curie University, Akademicka 19, 20-033 Lublin, Poland

autor

Krupa Z.

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