Borrelia burgdorferi infection among forestry workers - assessed with an immunoenzymatic method [ELISA], PCR, and correlated with the clinical state of the patients

• Autorzy: Niscigorska J. , Skotarczak B. , Wodecka B.
• Języki publikacji: EN

Abstrakty

EN

Occurrence of borreliosis in human population is associated with possibility of contact with the biological vector of this disease - a common European tick, Ixodes ricinus. Therefore, the highest number of cases of Lyme disease has been recorded among forestry workers and inhabitants of wooded areas. Diagnostics of borreliosis is based on immunoserologic tests - ELISA or indirect immunofluorescence method, Western blot technique, or on increasingly popular DNA examination using the polymerase chain reaction (PCR). In the present study, where 61% of the forestry workers were seropositive, we also tried to find a correlation between the results of serological tests and PCR tests with the clinical state of the patients. Despite finding IgM antibodies in 10 persons tested, which would indicate their recent infection, no DNA of B. burgdorferi s.l. was detected in their blood. Also, no DNA of this bacteria was present in 8 persons with IgM and IgG antibodies. No genetic material of the bacteria was found in persons with IgG antibodies, indicating the possibility of chronic infection. The clinical data suggested past symptomatic infection (ECM), or even more often, asymptomatic infection with B. burgdorferi.

Słowa kluczowe

EN

occupational hazard; serology; immunoenzymatic method; patient; infection; ELISA test; occupational medicine; polymerase chain reaction; Borrelia burgdorferi; borreliosis; forest worker

• Wydawca: -
• Czasopismo: Annals of Agricultural and Environmental Medicine
• Rocznik: 2003
• Tom: 10
• Numer: 1
• Opis fizyczny: p.15-19

Twórcy

autor

Niscigorska J.

• Pomeranian Medical University, Szczecin, Poland

autor

Skotarczak B.

autor

Wodecka B.

Bibliografia

• 1. Barciszewski J, Łastowski K, Twardowski T: Nowe tendencje w biologii molekularnej i inżynierii genetycznej oraz medycynie. T. 2. Sorus. Poznań 1996.
• 2. Berglund J, Eitrem R, Norrby S: Long-term study of Lyme borreliosis in a highly endemic area in Sweden. Scand J Infect Dis 1996, 28, 473-478.
• 3. Brettschneider S, Bruckbauer H, Klugbauer N, Hofmann H: Diagnostic value of PCR for detection of Borrelia burgdorferi in skin biopsy and urine samples from patients with skin borreliosis. J Clin Microbiol 1998, 36(9),2658-2665.
• 4. Brown CR, Reiner S: Genetic control of experimental Lyme arthritis in the absence of specific immunity. Infect Immun 1999, 67, 1967-1973.
• 5. Brown JP, Zachary J, Teuscher C, Weis J, Wooten R: Dual role of interleukin-10 in murine Lyme disease: regulation of arthritis severity and host defense. Infect Immun 1999, 67, 5142-5150.
• 6. Burgdorfer W, Barbour AG, Hayes SF, Benach JL, Grunwaldt E, Davies JP: Lyme disease: a tich-borne spirochetosis? Science 1982, 216, 1317-1319.
• 7. Carlson D, Hernandez J, Bloom BJ, Coburn J, Aversa JM, Steere AC: Lack of Borrelia burgdorferi DNA in synovial samples from patients with antibiotic treatment-resistant Lyme arthritis. Arthritis Rheum 1999, 42, 2705-2709.
• 8. Chmielewska-Badora J: Seroepidemiologic study on Lyme borreliosis in the Lublin region. Ann Agric Environ Med 1998, 5, 183-186.
• 9. Cisak E, Chmielewska-Badora J, Dutkiewicz J, Zwoliński J: Preliminary studies on the relationship between Ixodes ricinus activity and tick-borne infection among occupationally-exposed inhabitants of eastern Poland. Ann Agric Environ Med 2001, 8, 293-295.
• 10. Flisiak R, Prokopowicz D, Flisiak I, Bobrowska E, Męgoć H, Grzeszczuk A, Sulik E, Okołów C: Zagrożenia endemiczne boreliozą z Lyme w regionie Puszczy Białowieskiej. Przeg Epid 1994, 48, 210-217.
• 11. Grignolo MC, Buffrini L, Monteforte P, Rovetta G: Reliability of a polymerase chain reaction (PCR) technique in the diagnosis of Lyme borreliosis. Minerva Med 2001, 92, 29-33.
• 12. Guy EC, Stanek G: Detection of Borrelia burgdorferi in patients with Lyme disease by the polymerase chain reaction. J Clin Pathol 1991, 44(7), 610-611.
• 13. Harter L, Straubinger R, Summers B, Erb H, Appel M: Up- regulation of inducible nitric oxide synthase mRNA in dogs experimentally infected with Borrelia burgdorferi. Vet Immunol Immunopathol 1999, 67, 271-284.
• 14. Honegr K, Hulinska D, Dostal V, Gebousky P, Hankova E, Horacek J, Vysloužil L, Havlasova J: Persistence of Borrelia burgdorferi sensu lato in patients with Lyme borreliosis. Epidemiol Mikrobiol Imunol 2001,50, 10-16.
• 15. Hulínská D, Votypka J, Valešova M: Persistence of Borrelia garinii and Borrelia afzelii in patients with Lyme arthritis. Zentralbl Bakteriol 1999, 289(3), 301-318.
• 16. Januszkiewicz J, Kieda A: Przypadki boreliozy z Lyme na Pomorzu Zachodnim. Przeg Epidem 1987, 3, 324-329.
• 17. Kaiser R: Neuroborreliosis. J Neurol 1998, 245, 247-255.
• 18. Lebech AM, Hansen K, Brandrup F, Clemmensen O, Halkier- Sorensen L: Diagnostic value of PCR for detection of Borrelia burgdorferi DNA in clinical specimens from patients with erythema migrans and Lyme neuroborreliosis. Mol Diagn Jun 2000, 5(2), 139-150.
• 19. Levy S, Lissman B, Ficke C: Performance of a Borrelia burgdorferi bacterin in borreliosis-endemic areas. J Am Vet Med Assoc 1993, 202, 1834-1838.
• 20. Liang FT, Jacobson R, Straubinger R, Grooters A, Philipp M: Characterization of a Borrelia burgdorferi VisE Invariable Region Useful in Canine Lyme Disease Serodiagnosis by Enzyme-Linked Immunosorbent Assay. J Clin Microbiol 2000, 38, 4160-4166.
• 21. Liebling MR, Nishio MJ, Rodriguez A, Sigal LH, Jin T, Louie JS: The polymerase chain reaction for the detection of Borrelia burgdorferi in human body fluids. Arthritis Rheum 1993, 36, 665-675.
• 22. Lunemann JD, Zarmas S, Priem S, Franz J, Zschenderlein R, Aberer E, Klein R, Schouls L, Burmester GR, Krause A: Rapid typing of Borrelia burgdorferi sensu lato species in specimens from patients with different manifestations of Lyme borreliosis. J Clin Microbiol 2001, 39, 1130-1133.
• 23. Marconi RT, Garon CF: Development of polymerase chain reaction primer sets for diagnosis of Lyme disease and for species- specific identification of Lyme disease isolates by 16S rRNA signature nucleotide analysis. J Clin Microbiol 1992, 30(11), 2830-2834.
• 24. Mouritsen CL, Wittwer CT, Litwin CM, Yang L, Weis JJ, Martins TB, Jaskowski TD, Hill HR: Polymerase Chain Reaction Detection of Lyme Disease. Clin Microbiol Infect Dis 1996,105, 647-655.
• 25. Niscigorska J: Epidemiologic-clinical aspects of tick borne borreliosis in the Szczecin Province. Ann Acad Med Stetin 1999, 45, 157-173.
• 26. Niścigorska J: Ocena częstości występowania zakażeń Borrelia burgdorferi w województwie szczecińskim na podstawie badań immuno- serologicznych wybranych populacji. Prob Hig 1997, 54, 136-141.
• 27. Nohlmans M, van den Bogaard A, Blaauw A, van Boven C: Prevalence of Lyme borreliosis in The Netherlands. Ned Tijdschr Geneeskd 1991,135, 2288-2292.
• 28. Oksi J, Marttila H, Soini H, Aho H, Uksila J, Viljanen MK: Early dissemination of Borrelia burgdorferi without generalized symptoms in patients with erythema migrans. APMIS 2001,109, 581-588.
• 29. Ornstein K, Berglund J, Nilsson I, Norrby R, Bergstrom S: Characterization of Lyme borreliosis isolates from patients with erythema migrans and neuroborreliosis in southern Sweden. J Clin Microbiol 2001, 39, 1294-1298.
• 30. Pähl A, Kuhlbrandt U, Brune K, Rollinghoff M, Gessner A: Quantitative detection of Borrelia burgdorferi by real-time PCR. J Clin Microbiol 1999, 37, 1958-1963.
• 31. Pancewicz SA, Januszkiewicz A, Hermanowska-Szpakowicz T: Obecność przeciwciał przeciwko Borrelia burgdorferi wśród mieszkańców północno-wschodniej Polski. Przeg Epid 1996, 4, 375-381.
• 32. Pancewicz SA, Zajkowska J, Kondrusik M, Snarska-Furla I, Swierzbińska R Hermanowska-Szpakowicz T: Detection of antibodies to Borrelia burgdorferi among forestry workers in North-Eastern Poland. Med Pr 1998, 49, 253-259.
• 33. Pleyer U, Priem S, Bergmann L, Burmester G, Hartmann C, Krause A: Detection of Borrelia burgdorferi DNA in urine of patients with ocular Lyme borreliosis. Br J Ophthalmol 2001, 85, 552-555.
• 34. Priem S, Rittig MG, Kamradt T, Burmester GR, Krause A: An optimized PCR leads to rapid and highly sensitive detection of Borrelia burgdorferi in patients with Lyme borreliosis. J Clin Microbiol 1997, 35(3), 685-690.
• 35. Seiler K, Weis J: Immunity to Lyme disease: protection, pathology and peristence. Curr Opin Immunol 1996, 8, 503-509.
• 36. Skotarczak B, Wodecka В, Hermanowska Szpakowicz T: Czu³oœæ techniki PCR w wykrywaniu DNA Borrelia burgdorferi sensu lato w ró¿nych izolatach. Przeg Epidemiol 2002, 56, 73-79.
• 37. Stańczak J, Picken RN, Wegner Z, Kubica-Biernat B, Picken MM: Comparison of immunofluorescence assay and culture isolation for the detection of Borrelia burgdorferi sensu lato in Ixodes ricinus from northern Poland. VII International Congress on Lyme Borreliosis, San Francisco, 16-21 June 1996. Abstracts: 102. California 1996.
• 38. Steere A, Taylor E, Wilson M, Levin J, Spielman A: Longitudinal assessment of the clinical and epidemiological features of Lyme disease in defined population. J Infect Dis 1986, 200, 344-347.
• 39. Straubinger R, Straubinger A, Jacobson R, Chang Y-F, Summers B, Erb H, Appel M: Two Lessons From the Canine Model of Lyme Disease: Migration of Borrelia burgdorferi in Tissues and Persistence After Antibiotic Treatment. J Spirochetal Tick-Borne Dis 1997, 4, 24- 31.
• 40. Straubinger RK: PCR-Based quantification of Borrelia burgdorferi organisms in canine tissues over a 500-Day postinfection period. J Clin Microbiol 2000, 38(6), 2191-2199.
• 41. Weis JJ, McCracken В A, Ma Y, Fairbairn D, Roper RJ, Morrison TB, Weis JH, Zachary JF, Doerge RW, Teuscher C: Identification of quantitative trait loci governing arthritis severity and humoral responses in the murine model of Lyme disease. J Immunol 1999, 162, 948-956.