Inhibition of transcription starting from bacteriophage lambda pR promoter during the stringent response in Escherichia coli: implications for lambda DNA replication

• Autorzy: Szalewska-Palasz A. , Wegrzyn G.
• Języki publikacji: EN

Abstrakty

EN

Replication of X plasmid DNA is halted in amino acid-starved wild type (stringent) strains whereas it proceeds in relA (relaxed) mutants. The only transcription which could be important in X plasmid DNA replication in amino acid-starved Escherichia coli cells is that starting from the pR promoter. Using a fusion which consists of the lacZ gene under the control of bacteriophage X pR promoter we found that transcription starting from this promoter was inhibited during the stringent, but not the relaxed, response in E. coli. We confirmed our conclusion by estimating the relative level of the pR transcript by RNA-DNA hybridization. We propose that decreased transcription from the pR promoter which serves as transcriptional activation of oriX is responsible for inhibition of X plasmid replication during the stringent response. The results presented in this paper, combined with our recent findings (published elsewhere), indicate that the transcriptional activation of oriX may be a main regulatory process controlling X DNA replication not only during the relaxed response but also in normal growth conditions.

Słowa kluczowe

EN

bacteriophage; RNA; transcription; DNA replication; hybridization; regulation; Escherichia coli; stringent control

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1995
• Tom: 42
• Numer: 2
• Opis fizyczny: p.233-239,fig.

Twórcy

autor

Szalewska-Palasz A.

• University of Gdansk, Kladki 24, 80-822 Gdansk, Poland

autor

Wegrzyn G.

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