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2009 | 58 | 4 |

Tytuł artykułu

Highly thermostable exopolysaccharide produced by the moderately halophilic bacterium isolated from a man-made young salt lake in Romania

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Halophilic bacterial strains isolated from a man-made salt lake in Romania produce a specific exopolysaccharide. This product is synthesized under both static and stirring conditions, and the yield of this exoploysaccharide depends on the composition of the culture medium. The highest amount of polymer was obtained in the presence of casamino acids and yeast extract, under stirring conditions. This polymer has high thermostability, with a melting point at 207°C. The melting process is associated with the thermal degradation of the compound. This polymer is characterized by maximum absorbtion at 260 nm and fluorescence emission at 530 nm. The FT-IR analysis of the polymer revealed the presence of a saccharide structure and of amine and sulfate groups throughout the sugar backbone.

Wydawca

-

Rocznik

Tom

58

Numer

4

Opis fizyczny

p.289-294,fig.,ref.

Twórcy

autor
  • Romanian Academy, Splaiul Independentei 296, P.O.Box 56-53, Sector 6, Bucharest 060031, Romania
autor
autor
autor
autor
autor

Bibliografia

  • Arias S., A. del Moral, M.R. Ferrer, R. Tallon, E. Quesada and V. Bejar. 2003. Mauran, an exopolysaccharide produced by the halophilic bacterium Halomonas maura, with a novel composition and interesting properties for biotechnology. Extremophiles 7: 319-326.
  • Bejar V., I. Llamas, C. Calvo and E. Quesada. 1998. Characterization of exopolysaccharides produced by 19 halophilic strains included in the species Halomonas ewihalina. J. Biotechnol. 61: 135-141.
  • Bouchotroch S., E. Quesada, A. del Moral, I. Llamas and V. Béjar. 2001. Halomonas maura sp. nov., a novel moderately halophilic, exopolysaccharide-producing bacterium. Intl. J. Syst. Evol. Microbiol. 51: 1625-1632.
  • Calvo C, F. Martinez-Checa, A. Mota, V. Bejar and E. Quesada. 1998. Effect of cations, pH and sulphate content on the viscosity and emulsifying activity of the Halomonas eurihalina exopolysaccharide. J. Ind. Microbiol. Biotechnol. 20: 205-209.
  • Hasui M., M. Matsuda, K. Okutani and S. Shigeta. 1995. In vitro antiviral activities of sulfated polysaccharides from marine microalga (Cochlodiniumpolykrikoides) against human immunodeficiency virus and other enveloped virus. J. Biol. Macromol. 17: 293-297.
  • Hayashi K., T. Hayashi and I. Kojima. 1996. A natural sulfated polysaccharide calcium spirulan, isolated from Spirulinaplatensis: in vitro and anti-human immunodeficiency virus activities. Aids Res. Hum. Retroviruses. 12: 463-471.
  • Itoh H., H. Noda, H. Amano, C. Zhuaug, T. Mizuno and H. Ito.1993. Antitumor activity and immunological properties of marine algal polysaccharides, especially fucoidan, prepared from Sargassum thunbergii of Phaeophyceae. Anticancer Res. 13: 2045-2052.
  • Kushner D.J. and M. Kamekura. 1988. Physiology of halophilic Eubacteria. In: Rodriguez-Valera F (ed) Halophilic Bacteria, Vol. I. CRC Press, Inc. Boca Raton, Florida, pp. 109-138.
  • Margesin R. and F. Schinner. 2001. Potential of halotolerant and halophilic microorganisms for biotechnology. Extremophiles 5: 73-83.
  • Mata J.A., V. Béjar, I. Llamas, S. Arias, P. Bressollier, R. Tallon et al. 2006. Exopolysaccharides produced by the recently described halophilic bacteria Halomonas ventosae and Halomonas anticariensis. Research in Microbiol. 157: 827-835.
  • Philips R. de. and M. Vicenzini. 1998. Exocellular polysaccharides from cyanobacteria and their possible application. FEMS Microbiol. Rev. 22: 151-157.
  • Polz M.F. and M.C. Cavanaugh. 1998. Bias in Template-to-Product Ratios in Multitemplate PCR. Appl. Environ. Microbiol. 64: 3724-3730
  • Quesada E., V. Béjar and C. Calvo. 1993. Exopolysaccharide production by Volcaniella eurihalina. Experientia, 49: 1037-1041.
  • Riou D., S. Colliec-Jouault, D. Pinczon du Sel, S. Bosch, S. Siavoshian, V. Le Bert et al., 1996. Antitumor and antiproliferative effects of a fucan extracted from Ascophyllum nodosum against a non-small-cell bronchopulmonary carcinoma line. Anticancer Res. 16: 1213-1218.
  • Sutherland I.W. 1998. Novel and established applications of microbial polysaccharides. TIBTECH 16: 41-46.
  • Sutherland I.W. 1999. Microbial polysaccharide products. Biotechnol. Genet. Env. Rev. 16: 217-229.
  • Sutherland I.W. 2001. Microbial polysaccharides from Gram-negative bacteria. Intl. Dairy J. 11: 663-674.
  • Ventosa A., E. Quesada, F. Rodriguez-Valera, F. Ruiz-Berraquero and A. Ramos-Cormenzana. 1982. Numerical taxonomy of moderately halophilic Gram-negative rods. J. Gen. Microbiol. 128: 1959-1968.
  • Wilson K.H., R.B. Blitchington and R.C. Greene. 1990. Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction. J. Clin. Microbiol. 28: 1942-1946.
  • Witvrouw M. and E. de Clerq. 1997. Sulfated polysaccharides extracted from sea algae as potential antiviral drugs. Gen. Pharmacol. 29: 497-511.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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