Determination of IgG1, IgG2, and IgM antibody titres in the sera of bovine leukaemia virus - infected cows using the modified indirect ELISA

• Autorzy: Kozaczynska B
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to determine the titre of IgG₁, IgG₂, and lgM antibodies against bovine leukaemia virus (BLV) in the serum of either experimentally or naturally infected animals. Experimental infection performed in calves injected intravenously with blood from leukaemic cattle. The presence of BLV antibodies belonging to lgG₁, IgG₂, and IgM classes were studied for 7 months using the ELISA. Subsequent examinations were carried out on 126 cows from breeding stations extensively affected with BLV. Serological examinations of antibodies against BLV was performed by the ELISA. In the sera of experimentally infected animals IgM antibodies attained a maximum level in stage 2 (42 to 64 d) but lgG₁ antibodies during the last stage of immunogenesis ( 182 to 224 d). No statistically significant differences in IgG₂ antibodies throughout the entire experimental period were found. In the case of natural infection the IgG₁ and IgG₂ antibody levels increased concomitantly with the total antibody titres in the serum; however, IgG₂ values were significantly lower throughout the entire period of the study. In contrast, the highest lgM titre value was found in animals with the lowest level of total antibodies. Enzootic bovine leukaemia (EBL) is a neoplastic disease, associated with unlimited and irreparable proliferous alterations induced by bovine leukaemia virus (BL V) (25). Lymphocytes B are regarded as the target cells although T lymphocytes may also be infected (27, 30). Bovine leukosis reveals a long-lasting latency period involving integration of the viral genetic material with the host cell genome in the proviral form. A chronic lymphocytosis develops in about 30% of infected animals (1, 6, 27) whereas a small percentage of infected cattle develops tumours (6, 7). The production of antibodies against BLV is a permanent feature in the affected animals. The influence of BLV infection on immunoglobulin levels in sera has been extensively investigated but the results are contradictory (8, 9, 12, 13, 22, 29, 31). These investigations have mainly focused on total lg level measurements; consequently conflicting results concerning the behaviour of immunoglobulins regarded as the specific antibodies to the virus have been obtained.

Słowa kluczowe

EN

cow; leukemic cattle; serum; ELISA test; serological examination; animal infection; bovine leukemia virus; experimental infection; neoplastic disease; immunoglobulin level; enzootic bovine leukemia; antibody

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 1996
• Tom: 40
• Numer: 2
• Opis fizyczny: p.77-83,fig.

Twórcy

autor

Kozaczynska B

• National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

• 1. Abt D.A., Marshak R.R., Ferrer J.F., Piper C.E., Bhatt D.M.: Vet. Microbiol. 1, 287, 1976.
• 2. Atluru D., Johnson D.W., Paul P.S., Muscoplat C.C.: Am. J. vet. Res. 40, 515, 1979.
• 3. Bex F., Bruk C., Mammerickx M., Portetelle D., Ghysdael J., Cleuter Y., Leclercq M., Dekegel D., Burny A.: Cancer Res. 39, 1118, 1979.
• 4. Bicka L., Grundboeck-Juśko J., Kuzmak J., Jędrzejewska M., Kozaczyńska B.: Bull. vet. Inst. Pulawy 38, 81, 1993.
• 5. Deshayes L., Levy D., Parodi A. L., Levy J.P.: Int. J. Cancer 25, 503, 1980.
• 6. Ferrer J.F., Abt D.A., Bahatt D.M., Marshak R.R.: Cancer Res. 34, 893, 1974.
• 7. Ferrer J.F.: Adv. vet. Sci. comp. Med. 24, 1, 1980.
• 8. Gatei M.H., Brandon R.B., Naif H.M., Me Lennon M.W., Daniel R.C.W., Lavin M. F.: Vet. Immunol. Immunopath. 23, 139, 1989.
• 9. Gatei M.H., Lavin M.F., Daniel R. C.W.: J. vet. Med. B 37, 575, 1990.
• 10. Grundboeck-Juśko J., Grundboeck M., Bicka L., Kozaczyńska B., Stec J.: Bull. vet. Inst. Pulawy 30-31, 46, 1987-1988.
• 11. Grundboeck M., Grundboeck-Juśko J., Rułka J., Szczotka M.: Arch. exper. vet. Med. 40, 356, 1986.
• 12. Heeney J.L., Valli V., Montesanti J.: J. gen. Virol. 69, 659, 1988.
• 13. Ishihara K., Ohtani T., Kitagawa H.: Jap. J. vet. Sci. 42, 427, 1980.
• 14. Jacobs R.M., Valli V.E.O., Wilkie B.N.: Am. J. vet. Res. 41, 1942, 1980.
• 15. Klimentowski S.: Medycyna wet. 48, 56, 1992.
• 16. Kono Y., Hatakeyama H., Ishikawa H., Sentsui H.: Vet. Microbiol. 6, 167, 1981.
• 17. Kono Y., Sentsui H., Miyamoto T., Morozumi K., Sakamoto Y.: Int. J. Cancer 30, 655, 1982.
• 18. Kozaczyńska B.: Bull. vet. Inst. Pulawy 40, 17, 1996.
• 19. Kuźmak J., Grundboeck-Juśko J., Bicka L., Jędrzejewska M., Kozaczyńska B.: Bull. vet. Inst. Pulawy 38, 87, 1993.
• 20. Levy D., Deshayes L., Parodi A.L., Levy J.P.: Int. J. Cancer 25, 147, 1980.
• 21. Matthaeus W., Straub O.C.: Zentbl. Vet. Med. B 22, 758, 1975.
• 22. Matthaeus W., Straub O.C.: Vet. Microbiol. 1, 363, 1976.
• 23. Matthaeus W., Straub O.C.: Zbl. Bakteriol. Parasit. Infekt. Hyg. I. Abt. Orig. A. 240, 152, 1978.
• 24. Meiron R., Brenner J., Glukman A., Avraham R., Trainin Z.: Vet. lmmunol. Immunopath. 9, 105, 1985.
• 25. Miller J.M., Miller L.D., Olson C., Gillette K. G.: J. natl. Cancer Inst. 43, 1297, 1969.
• 26. Mussgay M., Kaaden O.R.: Curr. Top. Microbiol. Immunol. 79, 43, 1978.
• 27. Paul P.S., Pomeroy K.A., Johnson D.W., Muscoplat C.C., Handwerger B.S., Soper F.F., Sorensen D.K.: Am. J. vet. Res. 38, 873, 1977.
• 28. Pierce K.R., Young M.F., Mc Arthur N.H., Williams J. D.: Am. J. vet. Res. 38, 771, 1977.
• 29. Schultz R.D., Duncan J.R.: Fifth int. Symp. on Bovine Leukosis, CEC, Tubingen 1984.
• 30. Schwartz S., Felber B.K., Benko D.M., Fenyo E.M., Pavlakis G.N.: J.Virol. 64, 2519, 1990.
• 31. Sobiech E.: Praca doktorska. Akademia Rolnicza, Wrocław, 1979.
• 32. Trainin Z., Nobel T.A., Klopfer U., Neumann F.: Refuah vet. 5, 185, 1968.
• 33. Trainin Z., Mairom R., Klopfer U., Meidan G.: J. comp. Path. 83, 87, 1973.
• 34. Trainin Z., Ungar-Waron H., Meirom R., Barnea A., Sela M.: J. comp. Pathol. 86, 571, 1976.