Identification of a CAPA-PVK [Ixori-PVK] from single cells of the gulf Gulf Coast tick, Amblyomma maculatum

• Autorzy: Neupert S. , Russell W.K. , Russell D.H. , Strey O.F. , Teel P.D. , Strey A. , Nachman R.J.
• Języki publikacji: EN

Abstrakty

EN

MALDI-TOF/TOF tandem mass spectrometry has been applied to determine the complete sequence of a CAPA-PVK in the Gulf Coast tick, Amblyomma maculatum. Single cell analysis allowed the identification of the amino acid sequence of Ixori-PVK (PALIPFPRV-NH2), a periviscerokinin which had previously been identified from two other ticks, Ixodes ricinus and Boophilus microplus. The identification indicates greater conservation of sequence for the CAPA-PVK/CAP2b family in ticks as compared with insects. Side-chain fragmentation experiments provided data to distinguish between Leu/Ile ambiguities. The tick CAPA peptide shows a high sequence homology with other members of the insect periviscerokinin/ CAP2b peptides, which are associated with the regulation of critical physiological processes such as diuresis. Thus, the identification of this neuropeptide will provide the experimental basis to better understand regulation of water balance in these arthropods, providing a potential opportunity to develop neuropeptide-based control strategies against these livestock pests.

Słowa kluczowe

EN

diuresis; MALDI analysis; single cell; periviscerokinin; Boophilus microplus; Ixodidae; immunocytochemistry; Gulf Coast tick; amino acid sequence; MALDI-TOF mass spectrometry; neurohormone; Ixodes ricinus; Amblyomma maculatum

• Wydawca: -
• Czasopismo: Pestycydy
• Rocznik: 2008
• Numer: 1-2
• Opis fizyczny: p.67-73,fig.,ref.

Twórcy

autor

Neupert S.

• Friedrich-Schiller University, Erbertstrasse 1, 07745 Jena, Germany

autor

Russell W.K.

autor

Russell D.H.

autor

Strey O.F.

autor

Teel P.D.

autor

Strey A.

autor

Nachman R.J.

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