Evaluation of some immunoregulatory cytokines in serum of rats exposed to cadmium and ethanol

• Autorzy: Moniuszko-Jakoniuk J , Jurczuk M , Galazyn-Sidorczuk M
• Języki publikacji: EN

Abstrakty

EN

The aim of the present study was to investigate the effects of cadmium (Cd) (5 and 50 mg Cd/l in drinking water) and ethyl alcohol (ethanol, EtOH) (5 g EtOH/kg b.wt., intragastrically), administered alone or simultaneously, on the concentrations of pro-inflammatory (interleukin-1, IL-1?; interleukin-6, IL-6; tumor necrosis factor-?, TNF? and interferon ?, INF?) and anti-inflammatory (interleukin-4, IL-4) cytokines in the serum of rats. In order to estimate the involvement of Cd- or/and EtOH-induced oxidative stress in damage to cytokines, the concentration of protein carbonyl groups (PC), as a marker of oxidative protein damage, was also determined. Exposure to 5 and 50 mg Cd/l, alone or in combination with EtOH, led to an increase in the serum concentrations of IL-1?, TNF? and INF? with a simultaneous decrease in IL-4 concentration, compared to the control. The concentration of IL-6 was elevated only after administration of 50 mg Cd/l, both alone and in combination with EtOH. The exposure to EtOH alone resulted in increased concentrations of TNF? and INF?, as well as in decreased concentrations of IL-4. In rats co-exposed to Cd and EtOH, the changes observed in the concentrations of the cytokines, except in IL-6, were more advanced, compared to the animals treated with these xenobiotics alone. Exposure to Cd and EtOH, both alone and in combination, caused an increase in the serum PC concentration. The concentration of PC positively correlated with the concentrations of IL-1?, IL- 6, TNF? and INF? and negatively with IL-4 concentration. The results suggest that changes in the cytokines examined are more enhanced after combined exposure to Cd and EtOH, especially at higher Cd dosage. Moreover, it can be hypothesized that oxidative stress may be involved in the mechanism leading to changes in the concentration of cytokines after exposure to Cd and EtOH alone and in combination.

Słowa kluczowe

EN

cadmium; ethanol; cytokine; protein carbonyl group; rat; serum; immunoregulatory cytokine; drinking water; analytical procedure

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2009
• Tom: 18
• Numer: 4
• Opis fizyczny: p.673-680,fig.,ref.

Twórcy

autor

Moniuszko-Jakoniuk J

• Department of Toxicology, Medical University of Bialystok, Mickiewicza 2C, 15-222 Bialystok, Poland

autor

Jurczuk M

• Department of Toxicology, Medical University of Bialystok, Mickiewicza 2C, 15-222 Bialystok, Poland

autor

Galazyn-Sidorczuk M

• Department of Toxicology, Medical University of Bialystok, Mickiewicza 2C, 15-222 Bialystok, Poland

Bibliografia

• 1. ATSDR. Toxicological profile cadmium. Agency for Toxic Substances and Disease Registry, Atlanta, GA, 1999.
• 2. WORLD HEALTH ORGANISATION. Environmental Health Criteria 134, Cadmium, International Programme on Chemical Safety (IPCS), WHO, Geneva, 1992.
• 3. SARTARUG S., MOORE M. R. Adverse health effects of chronic exposure to low-level cadmium in foodstuffs and cigarette smoke. Environ. Health Perspect. 112, 1099, 2004.
• 4. OLSSON I. M., BENSRYD I., LUNDH T., OTTOSSON H., SKARFVING S., OSKARSSON A. Cadmium in blood and urine – impact of sex, age, dietary intake, iron status, and former smoking – association of renal effects. Environ. Health Perspect 110, 1185, 2002.
• 5. REHM J., ROOM R., GRAHAM K., MONTEIRO M., GMEL G., SEMPOS C. T. The relationship of average volume of alcohol consumption and patterns of drinking to burden of disease: an overview. Addiction 98, 1209, 2003.
• 6. MESSINGHAM K. A. N., FUANCE D. E., KOVACS E. J. Alcohol, injury, and cellular immunity. Alcohol 28, 137, 2002.
• 7. BRZÓSKA M. M., KAMIŃSKI M., SUPERNAK-BOBKO D., ZWIERZ K., MONIUSZKO-JAKONIUK J. Changes in the structure and function of the kidney of rats chronically exposed to cadmium. I. Biochemical and histopathological studies. Arch. Toxicol. 77, 344, 2003.
• 8. BRZÓSKA M. M., MONIUSZKO-JAKONIUK J. Bone metabolism of male rats chronically expose to cadmium. Toxicol. Appl. Pharmacol. 207, 195, 2005.
• 9. BRZÓSKA M. M., MONIUSZKO-JAKONIUK J., PIŁAT-MARCINKIEWICZ B., SAWICKI B. Liver and kidney function and histology in rats exposed to cadmium and ethanol. Alcohol Alcoholism, 38, 2, 2003.
• 10. TSHKAMOTO H., LU S. C. Current concepts in the pathogenesis of alcoholic liver injury. FASEB J. 15, 1335, 2001.
• 11. MARTH E. BARTH S., JELOVCAN S. Influence of cadmium on the immune system. Description of stimulating reactions. Cent. Eur. J. Public Health 8, 40, 2000.
• 12. DAN G., LALL S. B., RAO D. N. Humoral and cell mediated immune response to cadmium in mice. Drug Chem. Toxicol. 23, 349, 2000.
• 13. LAUFENETE A., GONZALEZ-CARRACEDO A., ESQUIFINO A. I. Differential effects of cadmium on blood lymphocyte subsets. BioMetals 17, 451, 2004.
• 14. LEFFEL E. K., WOLF C., POKLIS A., WHITE J. Drinking water exposure to cadmium, an environmental contamination, results in the exacerbation of autoimmune disease in the murine model. Toxicology 188, 233, 2003.
• 15. KATARANOVSKI M., KATARANOVSKI D., SAVIC D., JOVCIC G., BOGDANOVIC Z., JOVANOVIC T. Granulocyte and plasma cytokine activity in acute cadmium intoxication in rats. Physiol. Res. 47, 453, 1998.
• 16. GORAL J., KARAVITIS J. M., KOVACS E. J. Exposuredependent effects of ethanol on the innate immune system. Alcohol 42, 237, 2008.
• 17. SZABO G. Monocytes, alcohol use, and altered immunity. Alcohol. Clin. Exp. Res. 20, 216S, 1998.
• 18. STARKENBERG S., MUNROE M. E., WALTENBAUGH C. Early alteration in leukocyte population and Th1/Th2 function in ethanol-consuming mice. Alcohol. Clin. Exp. Res. 25, 1221, 2001.
• 19. CAO Q., BATEY R., PANG G., CLANCY R. Altered Tlymphocyte responsiveness to polyclonal cell activators is responsible for liver cell necrosis in alcohol-fed rats. Alcohol Clin. Exp. Res. 22, 723, 1998.
• 20. WĘGLARZ L., PARFINIEWICZ B., MERTAS A., KONDERA-ANASZ Z., JAWORSKA-KIK M., DZIERŻEWICZ Z., ŚWIĄTKOWSKA L. Effect of endotoxins isolated from Desulfovibrio desulfuricans soil and intestinal strain on the secretion of TNF-α by human mononuclear cells. Pol. J. Environ. Stud. 15, 615, 2006.
• 21. WĘGLARZ L., PARFINIEWICZ B., MERTAS A., KONDERA-ANASZ Z., JAWORSKA-KIK M., DZIERŻEWICZ Z. Effect of inositol hexsaphosphate on lipopolysaccharide-stimulated release of TNF-α from human mononuclear cells. Pol. J. Environ. Stud. 17, 283, 2008.
• 22. SHOOK L. B., LASKIN D. L. Xenobiotics and Inflammation. Academic Press, New York, 1994.
• 23. YUCESOY B., TURHAN A., URE M., IMIR T. KARAKAYA A. Effects of occupational lead and cadmium exposure on some immunoregulatory cytokine levels in man. Toxicology 123, 143, 1997.
• 24. SZUSTER-CIESIELSKA A., LOKAJ I., KANDEFER-SZERSZEŃ M. The influence of cadmium and zinc ions on the interferon and tumor necrosis factor production in bovine aorta endothelial cells. Toxicology 145, 135, 2000.
• 25. DANILUK J., SZUSTER-CIESIELSKA A., DRABKO J., KANDEFER-SZERSZEŃ M. Serum cytokine levels in alcohol-related liver cirrhosis. Alcohol 23, 29, 2001.
• 26. GONZALEZ-QUINTELA A., CAMPOS J., LOIDI L., QUINTERIO C., PEREZ L. F., GUDE F. Serum TNFα levels in relation to alcohol consumption and common TNF gene polymorphisms. Alcohol 42, 513, 2008.
• 27. DONG W., SIMEONOVA P. P., GALLUCCI R., MATHESON J., FLOOD L., WANG S., HUBBS A., LUSTER M. I. Toxic metals stimulate inflammatory cytokines in hepatocytes through oxidative stress mechanisms. Toxicol. Appl. Pharmacol. 151, 359, 1998.
• 28. ZHOU Z., WANG L., SONG Z., LAMBERT J. C., McCLAIN C. J., KANG J. A critical involvement of oxidative stress in acute alcohol-induced hepatic TNF-α production. Am. J. Pathol. 163, 1137, 2003.
• 29. WEELER M.D., KONO H., YIN M. The role of Kupffer cell oxidant production in early ethanol induced liver disease. Free Radic. Biol. Med. 31, 1544, 2001.
• 30. JURCZUK M., BRZÓSKA M. M., MONIUSZKO-JAKONIUK J., GAŁAŻYN-SIDORCZUK M., KULIKOWSKA-KARPIŃSKA E. Antioxidant enzymes activity and lipid peroxidation in liver and kidney of rats exposed to cadmium and ethanol. Food Chem. Toxicology 42, 429, 2004.
• 31. JURCZUK M., MONIUSZKO-JAKONIUK J., ROGALSKA J. Evaluation of oxidative stress in hepatic mitochondria of rats exposed to cadmium and ethanol. Pol. J. Environ. Stud. 15, 853, 2006.
• 32. JURCZUK M., MONIUSZKO-JAKONIUK J., ROGALSKA J. Glutathione-related enzyme activities in liver and kidney of rats exposed to cadmium and ethanol. Pol. J. Environ. Stud. 15, 861, 2006.
• 33. GAŁAŻYN-SIDORCZUK M., BRZÓSKA M. M., JURCZUK M., MONIUSZKO-JAKONIUK J. Oxidative damage to proteins and DNA in rats exposed to cadmium and/or ethanol. Chem. Biol. Interact. 180, 31, 2009.
• 34. HIRATSUKA H., SATOH S., SATOH M., NISHIJIMA M., KATSUKI Y., SUZUKI J., ANDO M. Tissue distribution of cadmium in rats given minimum amounts of cadmium-polluted rice or cadmium chloride for 8 months. Toxicol. Appl. Pharmacol. 160, 183, 1999.
• 35. WANG H., ZHU G., SHI Y., WENING S., JIN T., KONG Q., NORDBERG G. F. Influence of environmental cadmium exposure on forearm bone density. J. Bone Mineral. Res. 18, 553, 2003.
• 36. WIŚNIEWSKA-KNYPL J. M., WROŃSKA-NOFER T. Biological markers of oxidative stress induced by ethanol and iron overload in rats. Int. J. Occup. Med. Environ. Health 7, 355, 1994.
• 37. BANNO T., GAZEL A., BLUMENBERG M. Effects of tumor necrosis factor-alpha (TNF alpha) in epidermal keratinocytes revealed using global transcriptional profiling. J. Biol. Chem. 279, 32633, 2004.
• 38. BILLIAU A. Interferon-gamma: biology and role in pathogenesis. Adv. Immunol. 62, 61, 1996.
• 39. AREND W. P. The balance between IL-1 and IL-1R in disease. Cytokine Growth Factor Rev. 13, 323, 2002.
• 40. LOCKSLEY R. M., KILLEEN N., LENARDO M. J. The TNF and TNF receptor superfamiles: integrating mammalian biology. Cell 104, 487, 2001.
• 41. KAYAMA F., YOSHIDA T., ELWELL M. R., LUSTER M. I. Cadmium-induced renal damage and proinflammatory cytokines: possible role of IL-6 in tubular epithelial cell regeneration. Toxicol. Appl. Pharmacol. 134, 26, 1995.
• 42. MOSMANN T. R., SAD S. The expanding universe of Tcell subsets: Th1, Th2 and more. Immunol. Today 17, 138, 1996.
• 43. KROCOVA Z., MACELA A., KROCA M., HERNYCHOVA L. The immunomodulatory effect(s) of lead and cadmium on the cells of immune system in vitro. Toxicol In Vitro 14, 33, 2000.
• 44. SATOH M., KAJI T., TOHYAMA C. Low dose exposure to cadmium and its health effects. Part 3. Toxicity in laboratory animals and cultured cells. Nippon Eiseigaku Zasshi 57, 615, 2003.
• 45. TILG H., DIEHL A. M. Cytokines in alcoholic and nonalcoholic steatohepatitis. N. Engl. J. Med. 343, 1467, 2000.
• 46. LIN H. Z., YANG S. Q, ZELDIN G., DIEHL A. M. Chronic ethanol consumption induced the production of tumor necrosis factor-alpha and related cytokines in liver and adipose tissue. Alcohol Clin. Exp. Res. 22, 231S, 1998.
• 47. WORALL S., WILCE P. A. The effect of chronic ethanol feeding on cytokines in rat model of alcoholic liver disease. Alcohol Alcohol. Suppl. 2, 447, 1994.
• 48. GOLDSTEIN D. B. Ethanol-induced adaptation in biological membranes. Ann. N Y Acad. Sci. 492, 103, 1987.
• 49. HOEK J. B., RUBIN E. Alcohol and membrane-associated signal transduction. Alcohol Alcohol. 25, 143, 1990.
• 50. ZELDIN G., YANG S. Q., YIN M., LIN H. Z., RAI R., DIEHL A. M. Alcohol and cytokine-inducible transcription factors. Alcohol Clin. Exp. Res. 20, 1639, 1996.
• 51. McCLAIN C. J., SONG Z., BARVE S.S. HILL D. B., DEACIUC I. Recent advances in alcoholic liver disease. IV. Dysregulated cytokine metabolism in alcoholic liver disease. Am. J. Physiol Gastrointest. Liver Physiol. 287, G497, 2004.
• 52. WHEELER M. D., YAMASHINA S., FROH M., RUSYN I., THURMAN R. G. Adenoviral gene delivery can inactive Kupffer cells: role of oxidants in NF-κB activation and cytokine production. J. Leukoc. Biol. 69, 622, 2001.
• 53. YIN M., GABELE E., WHEELER M. D., CONNOR H., BRADFORD B. U., DIKALOVA A., RUSYN I., MASON R., THURMAN R. G. Alcohol-induced free radicals in mice: direct toxicants or signaling molecules? Hepatology 34, 935, 2001.