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2009 | 50 | 1 |

Tytuł artykułu

Isolation, sequence identification, and tissue expression profile of 3 novel porcine genes: NCF2, BCKDHB and BCKDHA

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The complete coding sequences of porcine genes NCF2, BCKDHB and BCKDHA were amplified by using reverse transcriptase polymerase chain reaction (RT-PCR), basing on the conserved coding sequence information of humans or other mammals. These 3 novel porcine genes were then assigned GeneIDs: 100142665, 100142669 and 100142666. The phylogenetic tree analysis revealed that the porcine NCF2, BCKDHB and BCKDHA all are most closely related to the bovine NCF2, BCKDHB and BCKDHA. Tissue expression profile analysis revealed that porcine NCF2, BCKDHB and BCKDHA genes were differentially expressed in tissues, including skeletal muscle, the heart, liver, fat, kidney, lung, small and large intestine.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

50

Numer

1

Opis fizyczny

p.47-50,fig.,ref.

Twórcy

autor
  • Key Laboratory of Animal Nutrition and Feed of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China

Bibliografia

  • Chinsky J, Appel M, Almashanu S, Costeas P, Ambulos N Jr, Carmi R, 1998. A nonsense mutation (R242X) in the branched-chain alpha-keto acid dehydrogenase E1 alpha subunit gene (BCKDHA) as a cause of maple syrup urine disease. Hum Mutat 12: 136.
  • Edelmann L, Wasserstein MP, Kornreich R, Sansaricq C, Snyderman SE, Diaz GA, 2001. Maple syrup urine disease: identification and carrier-frequency determination of a novel founder mutation in the Ashkenazi Jewish population. Am J Hum Genet 69: 863-868.
  • Flaschker N, Feyen O, Fend S, Simon E, Schadewaldt P, Wendel U, 2007. Description of the mutations in 15 subjects with variant forms of maple syrup urine disease. J Inherit Metab Dis 30: 903-909.
  • Gauss KA, Bunger PL, Crawford MA, McDermott BE, Swearingen R, Nelson-Overton LK, et al. 2006. Variants of the 5'-untranslated region of human NCF2: expression and translational efficiency. Gene 366: 169-179.
  • Hardison RC, 2003. Comparative genomics. PLoS Biol 1: E58.
  • Kang H, Lee SK, Cho SW, Lee SH, Kwack K, 2008. Branched chain alpha-keto acid dehydrogenase, El-beta subunit gene is associated with premature ovarian failure. Fertil Steril 89: 728-731.
  • Krötz F, Sohn HY, Gloe T, Zahler S, Riexinger T, Schiele TM, et al. 2006. Variants of the 5'-untranslated region of human NCF2: expression and translational efficiency. Gene 366: 169-179.
  • Lee PL, West C, Crain K, Wang L, 2006. Genetic polymorphisms and susceptibility to lung disease. J Negat Results Biomed 5: 5.
  • Liu GY, Xiong YZ, 2007. Isolation, sequence analysis and expression profile of a novel porcine gene, NIP7, differentially expressed in the longissimus dorsi muscle tissues from Meishan, Meishan Large White cross and Large White pigs. Mol Biol Rep 344: 213-219.
  • Liu YG, Xiong YZ, Deng CY, Zuo B, Zhang JH, 2004. Comparison of gene expression patterns in longissimus dorsi of pigs between the high-parent heterosis cross combination Landrace Large White and the mid-parent heterosis cross combination Large White Meishan. Asian-Aust J Anim Sci 17: 1192-1196.
  • Naik MT, Huang TH, 2004. Conformational stability and thermodynamic characterization of the lipoic acid bearing domain of human mitochondrial branched chain alpha-ketoacid dehydrogenase. Protein Sci 13: 2483-2492.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-cb818c1c-5556-4929-874d-4436c6ad8b33
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