Macrolide-lincosamide-streptogramin B resistant phenotypes and genotypes for methicillin-resistant Staphylococcus aureus in Turkey, from 2003 to 2006

• Autorzy: Gul H C , Kilic A. , Guclu A.U. , Bedir O. , Orhon M. , Basustaoglu C.
• Języki publikacji: EN

Abstrakty

EN

Methicillin-resistant Staphylococcus aureus (MRSA) strains with inducible macrolide-lincosamide-streptogramin B (iMLSB) resistance phenotype may lead to clinical failure during clindamycin (CLI) therapy. The aim of this study was to determine the incidence of MLS|t phenotypes by using D-test method and genotypes by using multiplex real-time PCR method in MRSA strains. A total of 265 MRSA strains were obtained from clinical samples from hospitalized and outpatients. Of the MRSA isolates, 225 (84.9%) were resistant to erythromycin (ERT), and 170 (64.1%) to CLI. Among the 225 ERT-resistant MRSA strains, the constitutive MLS|f (cMLSu) rate was found in 49.3%, iMLSB in 39.1% and the M phenotype in 11.5%. Overall, ermA, ermC, ermA+ermC, msrA, ermC+msrA, and ermA+ermC+msrA genes were detected in 85 (37.7%), 60 (26.6%), 42 (18.6%), 26 (11.5%), 11 (4.8%), and 1 (0.4%) isolates, respectively. Most prevalent resistance determinant in MRSA strains was ermA", which was detected in 37.7% of the isolates. The 26 MRSA strains with M phenotype harboured only msrA gene. In conclusion, due to aware of the potential of CLI treatment failure, D-test should be performed and reported in MRSA strains in clinical laboratories. The multiplex real-time PCR method is easy to perform, fast and reliable method for the detection of MLSB resistance genotypes.

Słowa kluczowe

EN

methicillin-resistant strain; health problem; clindamycin therapy; detection; phenotype; genotype; resistance genotype; multiplex real-time polymerase chain reaction; Staphylococcus aureus

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2008
• Tom: 57
• Numer: 4
• Opis fizyczny: p.307-312,ref.

Twórcy

autor

Gul H C

• Gulhane Military Medical Academy and School of Medicine, 06018, Etlik, Ankara, Turkey

autor

Kilic A.

autor

Guclu A.U.

autor

Bedir O.

autor

Orhon M.

autor

Basustaoglu C.

Bibliografia

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