EN
The aim of the study was to determine the immunological state of bitches suffering from pyometra. The study was performed on 87 bitches with open pyometra, 13 bitches with closed pyometra and 17 healthy control bitches. All bitches with pyometra were treated by ovario-hysterectomy. Prior to surgery, blood samples were collected from them to determine: w.b.c., r.b.c., Hb, Ht, differential w.b.c. A NBT reduction test and phagocytic index was also performed on the blood. In addition, the lysozyme activity, total protein level and globulin fraction were evaluated in the serum. C-reactive protein (CRP) was determined in 40 of the bitches. Uterus aspirates were collected from 68 bitches with open pyometra and 13 with closed pyometra for bacteriological examination. The study demonstrated that all immunological parameters in bitches with pyometra were lower compared to those of the control group. Only the CRP level was very high. In bitches with open pyometra the following aerobes were isolated: E. coli (78%), streptococci (13%), staphylococci (6%) and Pseudomonas aeruginosa (3%). No aerobes were isolated in bitches with closed pyometra.