Apoptosis and autophagy induced by TGF-beta1 in bovine mammary epithelial BME-UV1 cells

• Autorzy: Gajewska M. , Gajkowska B. , Motyl T.
• Języki publikacji: EN

Abstrakty

EN

Mammary gland growth and involution is based on a dynamic equilibrium between proliferation and apoptosis of mammary gland epithelial cells (MEC). TGF-ß1 is an important antiproliferative and apoptogenic factor for mammary gland epithelial cells, acting in auto/paracrine matter and thus considered an important local regulator of mammary tissue involution. So far the studies on mammary gland involution concerned only apoptosis as a type I of MEC programmed cell death (PCD). Autophagy is known to be type II of PCD and this paper is the first, supporting evidence for the TGF-ß1-induced autophagy in bovine mammary epithelial cell line BME-UV1, as a distinct to apoptosis type of PCD. Laser scanning cytometry and confocal microscopy were used for analysis of MAP1 LC3 and Beclin1 expression - two proteins considered being the most reliable biochemical markers of autophagy. The significant increase of MAP1 LC3 and Beclin1 expression in cells treated with TGF-ß1 (2 ng/ml) was observed. Ultrastructural observation in electron microscopy revealed that autophagy is not only alternative, but also complementary to apoptosis type of cell death in TGF-ß1-treated bovine MEC. It was manifested by typical morphological features of apoptosis (cell shrinkage, margination and condensation of chromatin) and autophagy (autophagosomes, autophagic vacuoles) in the same cell.

Słowa kluczowe

EN

mammary epithelial cell; epithelial cell; transforming growth factor-beta 1; BME-UV1 cell; apoptosis; mammary gland; autophagy

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology. Supplement
• Rocznik: 2005
• Tom: 56
• Numer: 3
• Opis fizyczny: p.143-157,fig.,ref.

Twórcy

autor

Gajewska M.

• Warsaw Agricultural University, Nowoursynowska 159, 02-787 Warsaw, Poland

autor

Gajkowska B.

autor

Motyl T.