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2008 | 64 | 11 |

Tytuł artykułu

Przypadek babeszjozy u konia

Warianty tytułu

EN
Babesiosis in a horse

Języki publikacji

PL

Abstrakty

EN
This paper describes a case of babesiosis in a horse in Poland. The horse was a 2-year-old stallion with the symptoms of fever, anemia, loss of appetite and muscle weakness. Blood samples were collected from the animal for the standard hematological and biochemical examinations, serological examination for Borreliae and molecular tests for Babesia spp. and Ehrichia spp. The results of hematological examinations revealed a low level of hemoglobin (11 g/dl), low level of hematocrit (28%), trombocythopenia (145 tys./mm³), and an increase of total bilirubine level, which are characteristic of hemolytic anemia. In PCR reaction with GF2 and GR2 primers a fragment of Babesia spp 18S RNA gene with the length of 577 bp was detected. The sequence showed a 96% homology with the Babesia equi partial sequence of 18S RNA gene with the GeneBank access number Z15105. On the basis of molecular examination results and the efficacy of a therapy with imidocarb in a dose of 2 ml/100 kg and kanamycin in a dose of 10 mg/kg the diagnose of babesiosis was established. A control PCR examination of the horse blood taken 14 days after finished therapy did not reveal the genetic material of Babesia. Full recovery of the sick animal was observed.

Wydawca

-

Rocznik

Tom

64

Numer

11

Opis fizyczny

s.1317-1319,fot.,tab.,bibliogr.

Twórcy

autor
  • Uniwersytet Przyrodniczy w Lublinie, ul.Gleboka 30, 20-612 Lublin

Bibliografia

  • 1. Adaszek Ł., Winiarczyk S.: Molecular characterization of Babesia canis canis isolates from naturally infected dogs in Poland. Vet. Parasitol. 2008, 152, 235-241.
  • 2. Alhassan A., Govind Y., Tam N. T., Thekisoe O. M., Yokoyama N., Inoue N., Igarashi I.: Comparative evaluation of the sensitivity of LAMP, PCR and in vitro culture methods for the diagnosis of equine piroplasmosis Parasitol. Res. 2007, 100, 1165-1168.
  • 3. Battsetseg B., Lucero S., Xuan X., Claveria F. G., Inoue N., Alhassan A., Kanno T., Igarashi I., Nagasawa H., Mikami T., Fujisaki K.: Detection of natural infection of Boophilus microplus with Babesia equi and Babesia caballi in Brazilian horses using nested polymerase chain reaction Vet. Parasitol. 2002, 107, 351-357.
  • 4. Camacho A. T., Guitian F. J., Pallas E., Gestal J. J., Olmeda A. S., Habela M. A., Telford S. R., Spielman A.: Theileria (Babesia) equi and Babesia caballi infections in horses in Galicia, Spain. Trop. Anim. Health Prod. 2005, 37, 293-302.
  • 5. Criado A., Martinez J., Buling A., Barba J. C., Merino S., Jefferies R., Irwin P. J.: New data on epizootiology and genetics of piroplasms based on sequences of small ribosomal subunit and cytochrome b genes. Vet. Parasitol. 2006, 142, 238-247.
  • 6. Heim A., Passos L. M., Ribeiro M. F., Costa-Júnior L. M., Bastos C. V., Cabral D. D., Hirzmann J., Pfister K.: Detection and molecular characterization of Babesia caballi and Theileria equi isolates from endemic areas of Brazil. Parasitol. Res. 2007, 102, 63-68.
  • 7. Kumar S., Gupta A. K., Pal Y., Dwivedi S. K.: In vivo therapeutic efficacy trial with artemisinin derivative, buparvaquone and imidocarb dipropionate against Babesia equi infection in donkeys. J. Vet. Med. Sci. 2003, 65, 1171-1177.
  • 8. Mehlhorn H., Schein E.: Redescription of Babesia equi Laveran, 1901 as Theileria equi. Parasitol. Res. 1998, 84, 467-475.
  • 9. Rampersad J., Cesar E., Campbell M. D., Samlal M., Ammons D.: A field evaluation of PCR for the routine detection of Babesia equi in horses. Vet. Parasitol. 2003, 114, 81-87.
  • 10. Štefančíková A., Adaszek Ł., Pet'ko B., Winiarczyk S., Dudiňák V.: Serological and immunochemical evidence of Borrelia burgdorferi sensu lato in horses and cattle from Poland and diagnostic problems of Lyme borreliosis. Ann. Agric. Environ. Med. (praca w druku).

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-c98ce9e2-5e75-469e-90ac-64ee2730994a
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