EN
This paper describes a case of babesiosis in a horse in Poland. The horse was a 2-year-old stallion with the symptoms of fever, anemia, loss of appetite and muscle weakness. Blood samples were collected from the animal for the standard hematological and biochemical examinations, serological examination for Borreliae and molecular tests for Babesia spp. and Ehrichia spp. The results of hematological examinations revealed a low level of hemoglobin (11 g/dl), low level of hematocrit (28%), trombocythopenia (145 tys./mm³), and an increase of total bilirubine level, which are characteristic of hemolytic anemia. In PCR reaction with GF2 and GR2 primers a fragment of Babesia spp 18S RNA gene with the length of 577 bp was detected. The sequence showed a 96% homology with the Babesia equi partial sequence of 18S RNA gene with the GeneBank access number Z15105. On the basis of molecular examination results and the efficacy of a therapy with imidocarb in a dose of 2 ml/100 kg and kanamycin in a dose of 10 mg/kg the diagnose of babesiosis was established. A control PCR examination of the horse blood taken 14 days after finished therapy did not reveal the genetic material of Babesia. Full recovery of the sick animal was observed.