The ELISA was used to measure anti-Pasteurella multocida IgG levels in the serum of 30 geese collected randomly from a 300-goose flock aged 2 months and vaccinated against pasteurellosis with the Pastivac E vaccine comprising Polish isolates of serotypes 1 and 3 with oil adjuvant. The blood was sampled every 14 days for 2 months from two groups of 10 birds each vaccinated singly and doubly and from non-vaccinated considered the controls. The ELISA kits were prepared by the author's procedure because no commercial kits for goose blood were available. An increase in anti-P. multocida IgG level was found after each vaccination.
1. Avakian A.P., Dick J.W., Deriux W.T., Henry C.W.: Comparison of various antigens and their ability to detect protective antibodies against Pasteurella multocida using enzyme-linked immunosorbent assay. Avian Dis. 1986, 30, 527-535.
2. Avakian A.P., Dick J.W., Derieux W.T.: Fowl cholera immunity induced by various vaccines in broiler minibreeder chickens determined by enzyme-linked immunosorbent assay. Avian Dis. 1989, 33, 97 - 101.
3. Briggs D.J., Skeeles J.K.: An enzyme-linked immunosorbent assay for detecting antibodies to Pasteurella multocida in chickens. Avian Dis. 1984, 28, 208 - 215.
4. Briggs D.J., Whitfill C.E., Skeeles J.K.: Probability of survival based on ELISA titer following challenge of vaccinated chickens with the X-73 strain of Pasteurella multocida. Avian Dis. 1985, 29, 904 - 911.
5. Carter G.R.: Studies on Pasteurella multocida. I. A hemagglutination test for identification of serological types. Am. J. Vet. Res. 1955, 16, 481 – 484.
6. Choi K.H., Maheswaran S.K., Molitor T.W.: Comparison of enzyme-linked immunosorbent assay with dot immunobinding assay for detection of antibodies against Pasteurella multocida in turkeys. Avian Dis. 1990, 34, 539 - 547.
7. Dick J.W., Johnson J.W.: Fowl cholera immunity in broiler breeder chickens determined by the enzyme-linked immunosorbent assay. Avian Dis. 1985, 29, 706 - 714.
8. Gardin Y., Huguet J.M., Bataille D.: Utilisation de la sérologic ELISA comme outil de suivi de la vaccination contre la Pasteurellose du canard. Revue Méd. Vét. 2000, 151, 57 - 62.
9. Heddleston K.L., Gallagher J.E., Rebers P.A.: Fowl cholera: gel diffusion precipitin test for serotyping Pasteurella multocida from avian species. Avian Dis. 1972, 16, 925 - 936.
10. Hofacre C.L., Glisson J.R., Kleven S.H.: Comparison of vaccination protocols of broiler breeder hens for Pasteurella multocida utilizing enzyme-linked immunosorbent assay and virulent challenge. Avian Dis. 1987, 31, 260 - 263.
11. Hogg A.: Mechanizm działania oraz możliwości zastosowania adiuwantu olejowego w produkcji szczepionek. Materiały z seminarium pt. Zastosowanie adiuwantów olejowych w celu zwiększenia skuteczności szczepionek. Iwet Puławy 1993, 32 -35.
12. Huang Z., Huang Y.X., Du W.X., Zhao X.X., Wu H.Q.: Measuring dynamic changes in antibodies against Pasteurella multocida in ducks with ELISA. Chinese J. vet. Sci. Technol. 1997, 27, 24 - 25.
13. Klaassen J.M., Bernard B.L., DiGiacomo R.F.: Enzyme-linked immunosorbent assay for immunoglobulin G antibody to Pasteurella multocida in rabbits. J. clin. Microbiol. 1985, 21, 617 - 621.
14. Marshall M.S., Robison R.A., Jensen M.M.: Use of an ezyme-linked immunosorbent assay to measure antibody responses in turkeys against Pasteurella multocida. Avian Dis. 1981, 25, 964 - 971.
15. Perelman B., Hadash D., Meroz M., Gur-Lavie A., Abramson M., Samberg Y.: Vaccination of young turkeys against fowl cholera. Avian Path. 1990, 19, 131 - 137.
16. Rutkowska-Jurga I.: Charakterystyka szczepów Pasteurella multocida występujących u drobiu oraz opracowanie szczepionki przeciw pasterelozie gęsi. Doctoral dissertation, PIWet Puławy 1999.
17. Samorek-Salamonowicz E., Czekaj H., Kozdruń W., Wilczyńska-Kowal M.: Wpływ immunostymulatorów na serokonwersję po szczepieniu przeciwko chorobie Derzsyego u gęsi. Medycyna Wet. 2000, 56, 103 - 106.
18. Sander J.E., Reynaldo S., Resurreccion W., Waltman D., McMurray B.L:. Pasteurella challenge and ELISA serology evaluation of broiler breeders vaccinated with live cholera vaccine. Avian Dis. 1998, 42, 190 - 193.
19. Tereszczukowa M., Truszczyński M., Rutowska-Jurga I.: Comparison of immunogenic properties of French Soma-Bacterine4 V vaccine with experimental vaccine against fowl cholera prepared in the Veterinary Research Institute. Bull. Vet Inst. Pulawy, 1982, 25, 1-4.
20. Truszczyński M., Tereszczukowa M., Rutkowska-Jurga I.: Wstępne badania nad czynnym uodpornianiem drobiu przeciw pasterelozie. Medycyna Wet. 1975, 31, 199 - 202.
21. Tsai H.J., Hwang M.J., Lu Y.S.: Enzyme-linked immunosorbent assay for antibodies to Pasteurella multocida in ducks. Taiwan J. Vet. Med. Anim. Husbandry 1995, 65, 139 - 146.
22. Westphal O., Jann K.: Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure. In: Methods in carbohydrate chemistry, Ed. Whistler R.L., Wolform M.L., Acad. Press, London, 1965, 83-91