The effects of disodium pamidronate on human polymorphonuclear leukocytes and platelets: an in vitro study

• Autorzy: Salvolini E , Orciani M. , Vignini A. , Di Primo R. , Mazzanti L.
• Języki publikacji: EN

Abstrakty

EN

Recent reports have indicated that, as well as having antiresorptive effects, bisphosphonates could have an application as anti-inflammatory drugs. Our aim was to investigate whether this anti-inflammatory action could be mediated by the nitric oxide (NO) released by the leukocytes migrating to the site of inflammation. In particular, we investigated in vitro the intracellular calcium concentration ([Ca2+]i), the level of NO released by PMN and platelets, and the PMN myeloperoxidase activity after incubation with disodium pamidronate, since there was a postulated modulatory effect of this aminosubstituted bisphosphonate on leukocytes both in vitro and in vivo. Our data shows that the pamidronate treatment provoked a significant increase in the [Ca2+]i parallel to the enhancement in NO release, suggesting a possible activation of constitutive nitric oxide synthase, while the myeloperoxidase activity was significantly reduced. In conclusion, we hypothesized that treatment with pamidronate could stimulate NO-production by cells present near the bone compartment, thus constituting a protective mechanism against bone resorption occurring during inflammation. In addition, PMN- and platelet-derived NO could act as a negative feed-back signal to restrict the inflammatory processes.

Słowa kluczowe

EN

disodium pamidronate; pamidronate; nitric oxide; calcium; intracellular calcium; man; polymorphonuclear leucocyte; leucocyte; platelet; in vitro condition; myeloperoxidase activity; reactive oxygen species

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2009
• Tom: 14
• Numer: 3
• Opis fizyczny: p.457-465,fig.,ref.

Twórcy

autor

Salvolini E

• Polytechnic University of Marche, via Tronto 10/A, 60020 Ancona, Italy

autor

Orciani M.

autor

Vignini A.

autor

Di Primo R.

autor

Mazzanti L.

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