Light pulse induces ALA-S gene expression in the rat Harderian gland

• Autorzy: Djeridane Y. , Lyoumi S. , Puy H. , Touitou Y.
• Języki publikacji: EN

Abstrakty

EN

The rodent Harderian glands (HGs) are large paired orbital organs with highest porphyrinogenic rates. We have previously shown that continuous light exposure abolished the day/night variations of the -aminolevulinate synthase (ALA-S; the rate-limiting enzyme for porphyrin biosynthesis) gene expression observed under standard light: dark cycles (LD 12:12) in the rat HGs. This study was designed to examine whether the ALA-S changes were actually associated directly with light. The response of ferrochelatase (enzyme that converts protoporphyrin IX into heme) to light was also examined. Male Wistar rats were acclimatized to light: dark cycles regimen of 12:12 for 2 weeks. At the end of the 2 weeks, a 1 h-light pulse was applied in the middle of the dark phase. Animals were sacrificed immediately after the end of the light pulse. HGs were collected and stored at -80°C until processed for quantitative RT-PCR. A 1 h-light pulse applied during mid-dark caused a significant increase of ALA-S gene expression (3-fold higher than in controls), whereas it was without effect on ferrochelatase gene expression. Our results suggest that light per se may regulate ALA-S gene expression in the rat HGs, and reveal that the ALA-S gene expression, and so heme biosynthesis, is under a photodynamic control.

Słowa kluczowe

EN

Harderian gland; rat; gene expression; rodent; light; porphyrin; delta-aminolevulinate synthase; ferrochelatase

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2010
• Tom: 61
• Numer: 1
• Opis fizyczny: p.115-117,fig.,ref.

Twórcy

autor

Djeridane Y.

• Faculte de Medecine Pierre et Marie Curie, Service de Biochimie Medicale et Biologie Moleculaire, INSERM UMRS 975, Paris ,France

autor

Lyoumi S.

autor

Puy H.

autor

Touitou Y.

Bibliografia

• Cardalda CA, Juknat AA, Princ FG, Batlle A. Rat Harderian gland porphobilinogen deaminase: characterization studies and regulatory action of protoporphyrin IX. Arch Biochem Biophys 1997; 347: 69-77.
• Lyoumi S, Martin-Schmitt C, Touitou Y, Puy H, Djeridane Y. Melatonin and environmental lighting regulate ALA-S gene expression and so porphyrin biosynthesis in the rat Harderian gland. Chronobiol Int 2008; 25: 851-867.
• Djeridane Y. The harderian gland of desert rodents: a histological and ultrastructural study. J Anat 1992; 180: 465-480.
• Djeridane Y. The Harderian gland and its excretory duct in the Wistar rat. A histological and ultrastructural study. J Anat 1994; 184: 553-566.
• Djeridane Y. Comparative histological and ultrastructural studies of the Harderian gland of rodents. Microsc Res Tech 1996; 34: 28-38.
• Portaluppi F, Touitou Y, Smolensky MH. Ethical and methodological standards for laboratory and medical biological rhythm research. Chronobiol Int 2008; 25: 999-1016.
• Wetterberg L, Geller E, Yuwiler A. Harderian gland: an extraretinal photoreceptor influencing the pineal gland in neonatal rats? Science 1970; 167: 884-885.
• Cui ZJ, Zhou YD, Satoh Y, Habara Y. A physiological role for protoporphyrin IX photodynamic action in the rat Harderian gland? Acta Physiol Scand 2003;179: 149-154.
• Hugo J, Krijt, Vokurka JM, Janousek V. Secretory response to light in rat Harderian gland: possible photoprotective role of Harderian porphyrin. Gen Physiol Biophys 1987; 6: 401-404.
• Joo I, Kahan A. The porphyrin content of harderian glands in rats and the melatonin-melanocyte stimulating hormone-system. Endokrinologie 1975; 65: 308-312.
• Reiter RJ, Klein DC. Observations on the pineal gland, the Harderian glands, the retina, and the reproductive organs of adult female rats exposed to continuous light. J Endocrinol 1971; 51: 117-125.
• Shirama K, Kohda M, Kohda M, Hokano M. Effects of lighting conditions and of hormone replacement on the level of porphyrins in the rat Harderian gland. J Endocrinol Invest 1987; 10: 79-82.
• Strum JM, Shear CR. Constant light exposure induces damage and squamous metaplasia in Harderian glands of albino mice. Tissue Cell 1982; 14: 149-161.
• Tomas-Zapico C, Coto-Montes A, Martinez-Fraga J, Rodriguez-Colunga MJ, Hardeland R, Tolivia D. Effects of d-aminolevulinic acid and melatonin in the Harderian gland of the Syrian hamster. Free Radic Biol Med 2002; 32: 1197-1204.
• Ulrich R, Yuwiler A, Geller E, Wetterberg L. Effects of sex hormones and environmental lighting on rat Harderian gland porphyrin. J Endocrinol 1974; 63: 99-102.
• Payne AP. The Harderian gland: a tercentennial review. J Anat 1994; 185: 1-49.