Plant regeneration in callus culture of Trifolium nigrescens Viv.

• Autorzy: Konieczny R.
• Języki publikacji: EN

Abstrakty

EN

This study was conducted to test the possibility of plant regeneration from callus tissue of Trifolium nigrescens Viv. Somatic embryos capable for development occurred when callus was removed from MS medium containing 8 mg/l 2,4-D and 2 mg/l kinetin and placed onto MS with 0,5 mg/l NAA and 2 mg/l 2iP. Embryoids induced directly on medium with NAA and 2iP displayed morphological abnormalities and never developed into plants. Organogenesis was observed when medium with 0,5 mg/l NAA and 2 mg/l 2iP was used. Somatic embryos and shoots were rooted on half-strength MS medium free of hormones. Plants obtained via somatic embryogenesis and organogenesis produced flowers and had typical morphological features.

Słowa kluczowe

EN

smallseeded legum; plant cytology; embryology; in vitro; Trifolium nigrescens; plant regeneration; cotyledon; somatic embryogenesis; organogenesis; explanting morphogenesis; Leguminosae; morphological feature; hypocotyl; callus tissue; somatic embryo

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 1995
• Tom: 37
• Opis fizyczny: p.47-52,fig.

Twórcy

autor

Konieczny R.

• Jagiellonian University, Grodzka 52, 31-044 Krakow, Poland

Bibliografia

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