Identification of zygotic and nucellar seedlings in Citrus interploid crosses by means of isozymes, flow cytometry and ISSR-PCR

• Autorzy: Tusa N , Abbate L. , Ferrante S. , Lucretti S. , Scarano M.T.
• Języki publikacji: EN

Abstrakty

EN

‘Milam’ (a purported hybrid of Citrus jambhiri Lush) + ‘Femminello’ lemon (Citrus limon L. Burm. f.) allotetraploid somatic hybrids were used as pollen parents in interploid crosses with diploid ‘Femminello’ lemon to achieve mal secco tolerance in different populations of seedless triploid lemon types with good fruit quality. A total of 137 plantlets were obtained and subjected to screening experiments, in order to distinguish zygotic embryos from nucellars. Here we report on and discuss the results obtained with three techniques: flow cytometry, isozyme analysis and ISSR-PCR (the inter-simple sequence repeats-polymerase chain reaction). ISSR-PCR resulted to be a very efficient and reliable technique for the identification of zygotic plantlets.

Słowa kluczowe

EN

interploid cross; Phoma tracheiphila; fruit quality; Femminello cultivar; lemon; Citrus jambhiri; hybrid; somatic hybrid; zygotic embryo; lemon cultivar; flow cytometry; back-crossing; fungal disease

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2002
• Tom: 07
• Numer: 2B
• Opis fizyczny: p.703-708,fig.

Twórcy

autor

Tusa N

• Istituto di Ricerca per la Genetica degli Agrumi - C.N.R. Viale delle Scienze n.11, 90128-Palermo, Italy

autor

Abbate L.

autor

Ferrante S.

autor

Lucretti S.

autor

Scarano M.T.

Bibliografia

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