Surface active properties of bacterial strains isolated from petroleum hydrocarbon-bioremediated soil

• Autorzy: Plaza G A , Ulfig K. , Brigmon R.L.
• Języki publikacji: EN

Abstrakty

EN

Two bacterial strains identified as Ralstonia picketti (BP 20) and Alcaligenes piechaudii (CZOR L-1B) were isolated from petroleum hydrocarbon-contaminated soil following bioremediation treatment. The surface active properties, e.g. surface tension, emulsification and foamability of their culture filtrates were evaluated. Bacterial cell-surface hydro-phobicity (BAH) as measured by analyzing cell affinity towards aliphatic and aromatic compounds was also determinated. The bacteria grew in liquid cultures containing 1% (v/v) of crude oil as carbon and energy source at 30°C under aerobic conditions. The surface tensions were reduced to 61 mN/m and 55 mN/m by Ralstonia picketti and Alcaligenes piechaudii, respectively. The emulsification index (EI24) was almost 100% for all tested compounds except diesel oil. The stability of the emulsions was determinated at 4°C, 45°C and 65°C. The emulsions were stable at 4°C. Ralstonia picketti was better foam inducer (FV = 50 ml) compared to Alcaligenes piechaudii (FV= 10 ml). The BAH measurements revealed higher adhesion of Alcaligenes piechaudii cells towards different hydrocarbons compared to Ralstonia picketti cells. The strains were found to have a surface hydrophobicity in the following order: aliphatic hydrocarbons, BTEX, and PAHs. The ability to adhere to bulk hydrocarbon is mostly a characteristic of hydrocarbon-degrading bacteria. The strains were found to be better emulsifiers than surface tension reducers. They produce water-soluble extracellular bioemulsifiers. Both bacterial isolates have good properties to use them, mainly in the petroleum industry, e.g. in enhanced oil recovery and in bioremediation processes-primarily due to their emulsification property, i.e. emulsion forming and stabilizing capacity.

Słowa kluczowe

EN

surface property; petroleum hydrocarbon-bioremediated soil; bioremediation; bacterial strain; Alcaligenes piechaudii; hydrocarbon; microorganism; bioemulsifier; biosurfactant; bacteria; microbiology; Ralstonia picketti

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2005
• Tom: 54
• Numer: 2
• Opis fizyczny: p.161-167,fig.,ref.

Twórcy

autor

Plaza G A

• Institute for Ecology of Industrial Areas, 40-844 Katowice, Poland

autor

Ulfig K.

autor

Brigmon R.L.

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