Resistance to Melampsora larici-epitea leaf rust in Salix: analyses of quantitative trait loci

• Autorzy: Ronnberg-Wastljung A -C , Samils B , Tsarouhas V , Gullberg U
• Języki publikacji: EN

Abstrakty

EN

Quantitative resistance of Salix to Melampsora larici-epilea leaf rust was studied in 2 Salix mapping populations. One population was a backcross between a S. schwerinii x S. viminalis hybrid and S. viminalis, and the other was an F₂ population between S. viminalis and S. dasyclados. A leaf disc bioassay was used to study the components of quantitative resistance (latent period, uredinia number, and uredinia size) to 3 isolates of the leaf rust. The analysis of quantitative trait loci (QTLs) revealed 9 genomic regions in the backcross population and 7 genomic regions in the F₂ population that were important for rust resistance, with QTLs explaining 8-26% of the phenotypic variation. An important genomic region was identified for the backcross population in linkage group 2, where QTLs were identified for all resistance components for 2 of the rust isolates. Four of the QTLs had overlapping mapping intervals, demonstrating a common genetic background for latent period, uredinia diameter, and uredinia number. QTLs specific to some rust isolates and to some resistance components were also found, indicating a combination of common and specific mechanisms involved in the various resistance components. Breeding implications in relation to these findings are discussed.

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2008
• Tom: 49
• Numer: 4
• Opis fizyczny: p.321-331,fig.,ref.

Twórcy

autor

Ronnberg-Wastljung A -C

• Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, Box 7080, SE-750 07 Uppsala, Sweden

autor

Samils B

• Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, Uppsala, Sweden

autor

Tsarouhas V

• Department of Developmental Biology, Wenner-Gren Institute, Stockholm University, Stockholm, Sweden

autor

Gullberg U

• Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, Box 7080, SE-750 07 Uppsala, Sweden

Bibliografia

• Åhman I, Larsson S, 1999. Resistensförädling i Salix för energiproduktion. Växtskyddsnotiser 2: 17-19.
• Åhman I, Bertholdsson N-O, 2001. Biomass willow cultivars and local variation in attacks by herbivores and rust. Asp Appl Biol 65: 205-214.
• Basten CJ, Weir BS, Zeng Z-B, 2001. QTL Cartographer, Version 1.15. Department of Statistics, North Carolina State University, Raleigh, NC.
• Beavis WD, 1994. The power and deceit of QTL experiments: lessons from comparative studies. Proceedings of the 49th Annual Corn And Sorghum Industry Research Conference, Chicago, IL: 250-266.
• Dowkiw A, Husson C, Frey P, Pinon J, Bastien C, 2003. Partial resistance to Melampsora larici-populina leaf rust in hybrid poplars: Genetic variability in inoculated excised leaf disk bioassay and relationship with complete resistance. Phytopathology 93: 421-427.
• Dowkiw A, Bastien C, 2004. Characterization of two major genetic factors controlling quantitative resistance to Melampsora larici-populina leaf rust in hybrid poplars: strain specificity, field expression, combined effects, and relationship with a defeated qualitative resistance gene. Phytopathology 94: 1358-1367.
• Dowkiw A, Bastien C, 2007. Presence of defeated qualitative resistance genes frequently has a major impact on quantitative resistance to Melampsora larici- populina leaf rust in P. inter americana hybrid poplars. Tree Genetics and Genomes 3: 261-274.
• Grattapaglia D, Sederoff R, 1994. Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-testcross: mapping strategy and RAPD markers. Genetics 137: 1121-1137.
• Jorge V, Dowkiw A, Faivre-Rampant P, Bastien C, 2005. Genetic architecture of qualitative and quantitative Melampsora larici-populina leaf rust resistance in hybrid poplar: genetic mapping and QTL detection. New Phytol 167: 113-127.
• Larsson S, 1998. Genetic improvement of willow for the short-rotation coppice. Biomass and Bioenergy 15: 23-26.
• Lascoux M, Ramstedth M, Åström B, Gullberg U 1996. Components of resistance of leaf rust (Melampsora laricii-epitea Kleb./Melampsora ribesii-viminalis Kleb.) in Salix viminalis L. Theor Appl Genet 93: 1310-1318.
• Lefèvre F, Pichot C, Pinon J, 1994. Intra- and interspecific inheritance of some components of the resistance to leaf rust (Melampsora larici-epitea Kleb.) in poplars. Theor Appl Genet 88: 501-507.
• Lefèvre F, Goué-Mourier MC, Faivre-Rampant P, Villar M, 1998. A single gene cluster controls incompatibility and partial resistance to various Melampsora larici-populina races in hybrid poplars. Phytopathology 88: 156-163.
• Legionnet A, Muranty H, Lefèvre F, 1999. Genetic variation of the riparian tree species Populus nigra. II. Variation in susceptibility to the foliar rust Melampsora larici-populina. Heredity 82: 318-327.
• McCracken AR, Dawson M, 1994. Effect of Melampsora rust on the growth and development of Salix burjatica ‘Korso’ in Northern Ireland. European Journal of Forest Pathology 24: 32-39.
• McCracken AR, Dawson M, 1998. Short rotation coppice willow in Northern Ireland since 1973: development of the use of mixtures in the control of foliar rust (Melampsora spp.). Eur J Forest Pathol 28: 241-250.
• McCracken AR, Dawson M, 2003. Rust disease (Melampsora epitea) of willow (Salix spp.) grown as short rotation coppice (SRC) in inter- and intra-species mixtures. Ann Appl Biol 143: 381-393.
• McDonald BA, Linde C, 2002. Pathogen population genetics, evolutionary potential and durable resistance. Annu Rev Phytopathol 40: 349-79.
• Newcombe G, 1998. Association of Mmd1, a major gene for resistance to Melampsora medusae f. sp. deltoidae, with quantitative traits in poplar rust. Phytopathology 88: 114-121.
• Pei MH, Royle DJ, Hunter T, 1996. Pathogen specialisation in Melampsora epitea var. epitea on Salix. Plant Pathol 45: 679-690.
• Pei MH, Yuan ZW, Hunter T, Ruiz C, 2000. Heterogeneous nature of a ‘new’ pathotype of Melampsora rust on Salix, revealed by AFLP. Eur J Plant Pathol 106: 771-779.
• Pinon J, van Dam BC, Genetet I, de Kam M, 1987. Two pathogenic races of Melampsora larici-populina in north-western Europe. Eur J Forest Pathol 17: 47-53.
• Pinon J, Frey P, 2005. Interactions between poplar clones and Melampsora populations and their implications for breeding for durable resistance. In: Pei M, McCracken AR, eds. Rust Diseases on Willow and Poplar. Wallingford UK: CAB International: 139-154.
• Rönnberg-Wästljung AC, Gunnerbeck E, 1985. Infektionsmönster och värdväxtskillnader vid Melampsora-angrepp i Salixodlingar. [Infection patterns and host differences in Melampsora attacks on Salix plantations]. Report 38. Dept, of Ecology and Environmental Sciences, Swedish University of Agricultural Sciences.
• Rönnberg-Wästljung A, Thorsén J, 1988. Inter- and intraspecific variation and genotype site interaction in Salix alba L., S. dasyclados Wimm. and S. viminalis L. Scand J Forest Res 3: 449—463.
• Rönnberg-Wästljung AC, Gullberg U, 1999. Genetics of breeding characters with possible effects on biomass production in Salix viminalis (L.). Theor Appl Genet 98: 531-540.
• Rönnberg-Wästljung AC, Tsarouhas V, Semerikov V, Lagercrantz U, 2003. A genetic linkage map of a tetraploid Salix viminalis and S. dasyclados hybrid based on AFLP markers. Forest Genet 10: 185-194.
• Rönnberg-Wästljung AC, Åhman I, Glynn C, Widenfalk O, 2006. Quantitative trait loci for resistance to herbivores in willow: field experiments with varying soils and climates. Entomol Exp Appl 118: 163-174.
• Samils B, Stepien V, Lagercrantz U, Lascoux M, Gullberg U, 2001. Genetic diversity in relation to sexual and asexual reproduction in populations of Melampsora larici-epitea. Eur J Plant Pathol 107: 871-881.
• Tsarouhas V, Gullberg U, Lagercrantz U, 2002. An AFLP and RFLP linkage map and quantitative trait loci (QTL) analysis of growth traits in Salix. Theor Appli Genet 105: 277-288.
• Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, et al. 1995. AFLP: a new technique for DNA fingerprinting. Nucl Acids Res 23: 4407-4414.