TG-repeat lenght polymorphism in the 5'-noncoding region of the growth hormone receptor gene in cattle and its association with meat production traits

• Autorzy: Maj A , Korczak M. , Oprzadek J. , Zwierzchowski L. , Dymnicki E.
• Języki publikacji: EN

Abstrakty

EN

A variable TG-repeat polymorphism was studied in the bovine growth hormone receptor (GHR) gene 5’-noncoding region. A total of seven alleles of the GHR gene were detected in six breeds. The length of the PCR-amplified variable region was from 306 to 320 bp, corresponding to 14 to 21 TG-repeats.Three additional alleles not reported so far, with 14, 19, and 21 TG-repeats were found. The associations between TG microsatellite genotypes and live body weight, daily live weight gain and carcass traits were studied in bulls of five beef breeds and Polish Black-and-White (Friesian) dairy cattle. In the latter the TG-repeat polymorphism in the 5’-noncoding region of the bovine gene GHR was significantly associated with mean body live weight, daily live weight gain, cold carcass weight and weight of lean and fat in valuable cuts. The longest 320-bp allele, with 21 TG repeats, was superior for most carcass traits, but when the growth rate was considered, the 320-bp allele homozygotes proved inferior to others.

PL

Badano polimorfizm długości powtórzeń dinukleotydu TG (mikrosatelity) w rejonie niekodującym 5’ genu receptora hormonu wzrostu (GHR) bydła. Analizując próbki DNA pochodzące od sześciu ras bydła stwierdzono łącznie siedem alleli genu GHR. Amplifikowany metodą PCR fragment DNA miał od 306 do 320 pz długości, co odpowiada 14 do 21 powtórzeń TG. Wykryto trzy nowe allele z 14, 19 i 21 powtórzeniami TG, nie opisane poprzednio w literaturze. Zbadano związek pomiędzy polimorfizmem genu GHR a masą ciała, tempem wzrostu i cechami jakości tuszy bydła mięsnego i polskiego bydła czarno-białego (cb). Wykazano istotny statystycznie (P≤0.05) związek ze średnimi dziennymi przyrostami, masą ciała, masą schłodzonej tuszy i masą chudego mięsa i tłuszczu w wartościowych wyrębach tuszy u bydła cb.„Najdłuższy” allel 320-pz, zawierający 21 powtórzeń TG, okazał się korzystny dla większości cech jakości tuszy, jednak homozygoty tego allelu wykazywały najmniejsze tempo wzrostu.

Słowa kluczowe

EN

polymorphism; gene; animal genetics; fattening; cattle; meat production trait; animal breeding; growth hormone; carcass

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2004
• Tom: 22
• Numer: 3
• Opis fizyczny: p.297-305,ref.

Twórcy

autor

Maj A

• Polish Academy of Sciences, Jastrzebiec, 05-552 Wolka Kosowska, Poland

autor

Korczak M.

autor

Oprzadek J.

autor

Zwierzchowski L.

autor

Dymnicki E.

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