The Escherichia coli RNA polymerase alpha subunit and transcriptional activation by bacteriophage lambda CII protein

• Autorzy: Gabig M. , Obuchowski M. , Ciesielska A. , Latala B. , Wegrzyn A. , Thomas M.S. , Wegrzyn G.
• Języki publikacji: EN

Abstrakty

EN

Bacteriophage λ is not able to lysogenise the Escherichia coli rpoA341 mutant. This mutation causes a single amino acid substitution Lys271Glu in the C-terminal domain of the RNA polymerase α subunit (αCTD). Our previous studies indicated that the impaired lysogenisation of the rpoA341 host is due to a defect in transcriptional activation by the phage CII protein and suggested a role for αCTD in this process. Here we used a series of truncation and point mutants in the rpoA gene placed on a plasmid to investigate the process of transcriptional activation by the CII gene product. Our results indicate that amino-acid residues 265, 268 and 271 in the α subunit may play an important role in the CII-mediated activation of the pE promoter (most probably residue 271) or may be involved in putative interactions between αCTD and an UP-like element near pE (most probably residues 265 and 268). Measurement of the activity of pE-lacZ, pI-lacZ and paQ-lacZ fusions in the rpoA+ and rpoA341 hosts demonstrated that the mechanism of activation of these CII-dependent promoters may be in each case different.

Słowa kluczowe

EN

bacteriophage; substitution; transcription; polymerase; Escherichia coli; RNA polymerase

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1998
• Tom: 45
• Numer: 1
• Opis fizyczny: p.271-280,fig.

Twórcy

autor

Gabig M.

• University of Gdansk, Kladki 24, 80-822 Gdansk, Poland

autor

Obuchowski M.

autor

Ciesielska A.

autor

Latala B.

autor

Wegrzyn A.

autor

Thomas M.S.

autor

Wegrzyn G.

Bibliografia

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