Wystepowanie bakterii chorobotworczych w tuszach wolowych i zwiazane z tym zagrozenie zdrowia konsumentow

• Autorzy: Wieczorek K , Denis E. , Osek J.

Warianty tytułu

EN

Occurrence of pathogenic bacteria in bovine carcasses and the related health threat to consumers

• Języki publikacji: PL

Abstrakty

EN

The aim of the study was to investigate the prevalence of human pathogenic bacteria Salmonella, L. monocytogenes, Campylobacter and verotoxigenic E. coli (VTEC) in bovine carcasses and to assess the related health threat to consumer. The examined material consisted of 276 samples collected as swabs with sterile sponges from carcasses directly after evisceration in slaughterhouses, from 400 cm² of the brisket area. The samples were analyzed at a laboratory for the identification of the four above-mentioned pathogens by slightly modified ISO methods and molecular techniques. The bovine carcasses were mostly contaminated with VTEC (30 positive samples, 10.9%) as found by the amplification of the Shiga toxin stx gene by the PCR technique. The other microorganisms were identified in smaller proportions of the carcasses. Eight samples (2.9%) proved positive for Campylobacter spp., 7 (2.5%) for L. monocytogenes, and 5 (1.8%) were contaminated with Salmonella spp. The influence of the animals’ age on the carcass contamination was also analyzed: it was noted that the majority of isolates were present in samples recovered from cattle over 6 years old. Moreover, the influence of seasonality on the occurrence of the pathogens in the carcasses was tested, and it was demonstrated that the majority of L. monocytogenes were isolated in October and November. In the case of VTEC, most of the bacteria were isolated from the carcasses in October, November and December (83.3% of the isolates) whereas no VTEC was found in winter (January-March) and spring (April-June). Most of the Salmonella spp. positive samples (4 out of 5 carcasses) were found in the period of May-July. No seasonality was found in the case of Campylobacter spp. present in the bovine carcasses tested. The results of the study show that bovine carcasses are quite frequently contaminated with pathogenic microorganisms which may be a source of food-borne diseases. Taking this into account, proper hygiene throughout the entire food chain, especially at the stage of carcass processing in slaughterhouses, is necessary to protect the health of consumers.

Słowa kluczowe

PL

zywnosc; mieso wolowe; tusze wolowe; zanieczyszczenia bakteryjne; bakterie chorobotworcze; Campylobacter; Salmonella; Listeria monocytogenes; Escherichia coli; szczepy shigatoksyczne; wystepowanie; sezonowosc; identyfikacja; konsumenci; zagrozenia zdrowia

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2010
• Tom: 66
• Numer: 01
• Opis fizyczny: s.54-58,tab.,bibliogr.

Twórcy

autor

Wieczorek K

• Panstwowy Instytut Weterynaryjny - Panstwowy Instytut Badawczy, Al.Partyzantow 57, 24-100 Pulawy

autor

Denis E.

autor

Osek J.

Bibliografia

• 1. Bacon R. T., Sofos J. N., Belk K. E., Hyatt D. R., Smith G. C.: Prevalence and antibiotic susceptibility of Salmonella isolated from beef animal hides and carcasses. J. Food Prot. 2002, 65, 284-290.
• 2. Barkocy-Gallagher G. A., Arthur T. M., Rivera-Betancourt M., Nou X., Shackelford S. D., Wheeler T. L., Koohmaraie M.: Seasonal prevalence of Shiga toxin-producing Escherichia coli, including O157:H7 and non-O157 serotypes, and Salmonella in commercial beef processing plants. J. Food Prot. 2003, 66, 1978-1986.
• 3. Beach J. C., Murano E. A., Acuff G. R.: Prevalence of Salmonella and Campylobacter in beef cattle from transport to slaughter. J. Food Prot. 2002, 65, 1687-1693.
• 4. Bettelheim K. A.: Role of non-O157 VTEC. Symp. Ser. Soc. Appl. Microbiol. 2000, 29, 38-50.
• 5. Cobbold R. N., Davis M. A., Rice D. H., Szymanski M., Tarr P. I., Besser T. E., Hancock D. D.: Associations between bovine, human, and raw milk, and beef isolates of non-O157 Shiga toxigenic Escherichia coli within a restricted geographic area of the United States. J. Food Prot. 2008, 71, 1023-1027.
• 6. Ghafir Y., China B., Dierick K., De Zutter L., Daube G.: A seven-year survey of Campylobacter contamination in meat at different production stages in Belgium. Int. J. Food Microbiol. 2007, 116, 111-120.
• 7. Gyles C. L: Shiga toxin-producing Escherichia coli: an overview. J. Anim. Sci. 2007, 85, 45-62.
• 8. Hancock D., Besser T., Lejeune J., Davis M., Rice D.: The control of VTEC in the animal reservoir. Int. J. Food Microbiol. 2001, 66, 71-78.
• 9. Jelacic J. K., Damrow T., Chen G. S., Jelacic S., Bielaszewska M., Ciol M., Carvalho H. M., Melton-Celsa A. R., O'Brien A. D., Tarr P. I.: Shiga toxin-producing Escherichia coli in Montana: bacterial genotypes and clinical profiles. J. Infect. Dis. 2003, 188, 719-729.
• 10. Johnson K. E., Thorpe C. M., Sears C. L.: The emerging clinical importance of non-O157 Shiga toxin-producing Escherichia coli. Clin. Infect. Dis. 2006, 43, 1587-1595.
• 11. Korsak N., Daube G., Ghafir Y., Chahed A., Jolly S., Vindevogel H.: An efficient sampling technique used to detect four foodborne pathogens on pork and beef carcasses in nine Belgian abattoirs. J. Food Prot. 1998, 61, 535-541.
• 12. Mayrhofer S., Paulsen P., Smulders F. J., Hilbert F.: Antimicrobial resistance profile of five major food-borne pathogens isolated from beef, pork and poultry. Int. J. Food Microbiol. 2004, 97, 23-29.
• 13. McEvoy J. M., Doherty A. M., Sheridan J. J., Blair I. S., McDowell D. A.: The prevalence of Salmonella spp. in bovine faecal, rumen and carcass samples at a commercial abattoir. J. Appl. Microbiol. 2003, 94, 693-700.
• 14. Osek J.: Identyfikacja shigatoksycznych Escherichia coli w tuszach wołowych metodą PCR. Medycyna Wet. 2008, 64, 179-182.
• 15. Osek J.: Występowanie chorób odzwierzęcych i ich czynników etiologicznych w 2006 r. w świetle raportu Europejskiego Urzędu do spraw Bezpieczeństwa Żywności. Życie Wet. 2008, 83, 192-201.
• 16. Pezzotti G., Serafin A., Luzzi I., Mioni R., Milan M., Perin R.: Occurrence and resistance to antibiotics of Campylobacter jejuni and Campylobacter coli in animals and meat in northeastern Italy. Int. J. Food Microbiol. 2003, 82, 281-287.
• 17. Phillips D., Sumner J., Alexander J. F., Dutton K. M.: Microbiological quality of Australian beef. J. Food Prot. 2001, 64, 692-699.
• 18. Rivera-Betancourt M., Shackelford S. D., Arthur T. M., Westmoreland K. E., Bellinger G., Rossman M., Reagan J. O., Koohmaraie M.: Prevalence of Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella in two geographically distant commercial beef processing plants in the United States. J. Food Prot. 2004, 67, 295-302.
• 19. Samadpour M., Barbour M. W., Nguyen T., Cao T. M., Buck F., Depavia G. A., Mazengia E., Yang P., Alfi D., Lopes M., Stopforth J. D.: Incidence of enterohemorrhagic Escherichia coli, Escherichia coli O157, Salmonella, and Listeria monocytogenes in retail fresh ground beef, sprouts, and mushrooms. J. Food Prot. 2006, 69, 441-443.
• 20. The Community Summary Report on Trends and Sources of Zoonoses and Zoonotic Agents in the European Union in 2007. http://www.efsa.europa.eu.
• 21. Uradziński J., Wysok B.: Zagrożenia bezpieczeństwa żywności powodowane czynnikami zoonotycznymi. Medicina Veterinaria 2008, 7, 15-22.
• 22. Wieczorek K., Osek J.: Testy multiplex PCR do równoczesnej identyfikacji Campylobacter jejuni i Campylobacter coli. Medycyna Wet. 2005, 61, 797-799.