Application of mitochondrial DNA in the identification of diverse crayfish species

• Autorzy: Soroka M

Warianty tytułu

PL

Wykorzystanie mitochondrialnego DNA w identyfikacji roznych gatunkow rakow

• Języki publikacji: EN

Abstrakty

EN

The populations of two indigenous Polish species, the noble crayfish (Astacus astacus) and the narrow-clawed crayfish (Astacus leptodactylus), were gradually reduced over the 20th century by extensive water pollution, the subsequent eutrophication over time of lakes and rivers, and the epizooty plague caused by the fungus Aphanomyces astaci. The crayfish populations were also adversely affected by the introduction of the American crayfish in 1890 (Orconectes limosus) and the signal crayfish (Pacifastacus lenisculus) in the late 1960s. This work aims at finding molecular markers that would permit objective identification of crayfish species. This could facilitate monitoring the distribution and protection of crayfish species, both in Poland and throughout the world. To this end, the PCR-RFLP technique was used for the analysis of the mitochondrial coxl gene, using 5 restriction enzymes. The best molecular marker was the restriction enzyme AluI, which proved efficient in differentiating all the studied crayfish. Five unique genotypes that occurred in a single species were observed, with most of them in the enzyme AluI, which therefore may be regarded as a species-diagnostic marker.

PL

Rak szlachetny (Astacus astacus) i rak błotny (Astacus leptodactylus) należą do rodzimych gatunków raków, których populacje pod względem ilości i liczebności ulegają systematycznemu zmniejszeniu od XIX w. Przyczyną tego jest postępująca eutrofizacja wód i ich zanieczyszczenie, powtarzające się epizoocje dżumy raczej oraz intensywne zasiedlanie stanowisk występowania raków przez dwa introdukowane z Ameryki Północnej gatunki, raka pręgowatego (Pacifastacus leniusculus) i sygnałowego (Orconectes limosus). Program ochrony i restytucji raków w Polsce wymaga opracowania skutecznych metod ich identyfikacji gatunkowej na różnych etapach rozwoju osobniczego. Przeprowadzone w pracy analizy PCR-RFLP dla genu cox1 u czterech gatunków raków (A. astacus, A. leptodactylus oraz P. leniusculus, O. limosus) pozwoliły na opracowanie markerów RFLP do identyfikacji taksonomicznej. Najbardziej skuteczny okazał się enzym restrykcyjny AluI, którego efekt trawienia jest diagnostyczny dla analizowanych gatunków. Z czterech badanych gatunków raków najłatwiejszy do identyfikacji okazał się P. leniusculus posiadający największą liczbę genotypów unikalnych.

• Wydawca: -
• Czasopismo: Polish Journal of Natural Sciences
• Rocznik: 2008
• Tom: 23
• Numer: 3
• Opis fizyczny: p.624-634,fig.,ref.

Twórcy

autor

Soroka M

• University of Szczecin, Piastow 40b, 71-065 Szczecin, Poland

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