Labile iron pool correlates with iron content in the nucleus and the formation of oxidative DNA damage in mouse lymphoma L5178Y cell lines

• Autorzy: Kruszewski M. , Iwanenko T.
• Języki publikacji: EN

Abstrakty

EN

Labile iron pool (LIP) constitutes a crossroad of metabolic pathways of iron-containing compounds and is midway between the cellular need for iron, its uptake and storage. In this study we investigated oxidative DNA damage in relation to the labile iron pool in a pair of mouse lymphoma L5178Y (LY) sublines (LY-R and LY-S) differing in sensitivity to hydrogen peroxide. The LY-R cells, which are hydro­gen peroxide-sensitive, contain 3 times more labile iron than the hydrogen perox­ide-resistant LY-S cells. Using the comet assay, we compared total DNA breakage in the studied cell lines treated with hydrogen peroxide (25 uM for 30 min at 4°C). More DNA damage was found in LY-R cells than in LY-S cells. We also compared the levels of DNA lesions sen­sitive to specific DNA repair enzymes in both cell lines treated with H2O2. The levels of endonuclease Ill-sensitive sites and Fapy-DNA glycosylase-sensitive sites were found to be higher in LY-R cells than in LY-S cells.

Słowa kluczowe

EN

lymphoma; cell line; mouse; mice; hydrogen peroxide; iron homeostasis; DNA damage; iron content; labile iron pool; nucleus; iron-containing compound

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 1
• Opis fizyczny: p.211-215,fig.

Twórcy

autor

Kruszewski M.

• Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warsaw, Poland

autor

Iwanenko T.

Bibliografia

• Beer JZ, Budzicka E, Niepokojczycka E, Rosiek O, Szumiel I, Walicka M. (1983) Cancer Res.; 43: 4736-42.
• Bouzyk E, Buraczewska I, Rosiek O, Sochanowicz B, Szumiel I. (1991) Radiat Environ Biophys.; 30: 105-16.
• Bouzyk E, Iwanenko T, Jarocewicz N, Kruszewski M, Sochanowicz B, Szumiel I. (1997) Free Radic Biol Med. ; 22: 697-704.
• Breuer W, Epsztejn S, Cabantchik ZI. (1996) FEBSLett; 382: 304-8.
• Breuer W, Greenberg E, Cabantchik ZI. (1997) FEBS Lett.; 403: 213-9.
• Emerit J, Beaumont C, Trivin F. (2001) BiomedPharmacother.; 55: 333-9.
• Epsztejn S, Kakhlon O, Glickstein H, Breuer W, Cabantchik I. (1997) Anal Biochem.; 248: 31-40.
• Gackowski D, Kruszewski M, Bartlomiejczyk T, Jawien A, Ciecierski M, Olinski R. (2002) J Biol Inorg Chem.; 7: 548-50.
• Henle ES, Luo Y, Gassmann W, Linn S. (1996) J Biol Chem.; 271: 21177-86.
• Konijn AM, Glickstein H, Vaisman B, Meyron-Holtz EG, Slotki IN, Cabantchik ZI. (1999) Blood.; 94: 2128-34.
• Kruszewski M, Szumiel I. (1994) Acta Biochim Polon.; 41: 120-1.
• Kruszewski M, Wojewodzka M, Iwanenko T, Collins AR, Szumiel I. (1998) MutatRes.; 416: 37-57.
• Lai CC, Huang WH, Klevay LM, Gunning WT, III, Chiu TH. (1996) Free Radic Biol Med.; 21: 233-40.
• Lipinski P, Drapier JC, Oliveira L, Retmanska H, Sochanowicz B, Kruszewski M. (2000) Blood.; 95: 2960-6.
• McCord JM. (1998) Semin Hematol.; 35: 5-12.
• Meneghini R. (1997) Free Radic Biol Med.; 23: 783-92.
• Mori T, Dizdaroglu M. (1994) Radiat Res.; 140: 85-90.
• Petrat F, De Groot H, Rauen U. (2001) Biochem J. ; 356: 61-9.
• Szumiel I. (1979) Chem-Biol Interact.; 24: 51-72.
• Szumiel I, Kapiszewska M, Kruszewski M, Iwanenko T, Lange CS. (1995) Radiat Environ Biophys.; 34: 113-9.
• Touati D. (2000) Arch Biochem Biophys.; 373: 1-6.
• Zastawny TH, Kruszewski M, Olinski R. (1998) Free Radic Biol Med.; 24: 1250-5.