Adhezyny paleczek z rodzaju Acinetobacter

• Autorzy: Fleischer M. , Przondo-Mordarska A.
• Języki publikacji: PL

Abstrakty

PL

Obecność i typ fimbrii występujących u czterech gatunków rodzaju Acinetobacter: A. baumannii, A. junii, A. lwoffii i A. haemolyticus określono testem hemaglutynacji z 3% zawiesiną świeżych i taninowanych erytrocytów świnki morskiej, bydlęcych oraz ludzkich grupy O i AB bez, lub z dodatkiem inhibitorów cukrowych. U szczepów każdego z gatunków dominowały he- maglutyniny MR odpowiadające fimbriom typu 3 obok których, zwłaszcza u szczepów izolowanych z układu moczowego i oddechowego, występowały dodatkowe adhezyny o różnej swoistości cukrowej.

EN

One of the important factors contributing to the pathogenicity of bacteria is the presence of adhesines on cell surface, which facilitate colonisation in the macroorganism. The presence and type of adhesines occurring in four species of the genus Acinetobacter: A. baumannii (184), A. junii (59), A. lwoffii (65) and A. haemolyticus (22) was determined by haemagglutination test with a 3% suspension of fresh, tannic acid-treated of guinea pig, cow and human group O and AB erythrocytes, with or without the addition of one of sugar inhibitors (D- mannose, α-methylmannopyranoside, D-galactose-N-acetyl-D-glucosamine, L-fucose and D-ribose). In strains from all species, adhesines of the mannose-resistant (MR) type dominated. The mannose-sensitive (MS) type was present solely on the surface of one A. Iwoffi strains. A. baumannii (36), A. junii (8), A. Iwoffii (11) and A. haemolyticus (4) exhibited mannose-resistant hemagglutination in relation to fresh erythrocytes and that reaction was restrained by D-galactose, D-galactose and L-fucose (no other inhibitor used restrained it). The results achieved prove that cell adhesines other than those of MR type must be present on the cell surface. Additional adhesines occurred mainly in strains isolated from the respiratory and urinary tract infection simples, but were not found in isolates from blood cultures.

Słowa kluczowe

PL

Acinetobacter baumannii; Acinetobacter haemolyticus; Acinetobacter junii; adhezja bakterii; fimbrie; test hemaglutynacji; oznaczanie; bakterie; adhezyny; Acinetobacter lwoffii

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 1998
• Tom: 50
• Numer: 3-4
• Opis fizyczny: s.229-237,tab.,bibliogr.

Twórcy

autor

Fleischer M.

• Akademia Medyczna, 50-368 Wroclaw, ul.Chalubinskiego 4

autor

Przondo-Mordarska A.

Bibliografia

• 1. Andreoni S. Adhesive properties of Enterobacteriaceae. Alpe Adria Micr J 1994; 3: 159-73.
• 2. Beachey EH, Abraham SN. Biological properities of bacterial surface proteins: type 1 fim¬briae of Escherichia coli. in: Schrinner E, Richmond PMM, Seibert G, Schwarz U (eds.) Surface structures of microorganisms and their interaction with the mammalian host. VCH. Weinheim, 1988.
• 3. Bergogne-Berezin E, Joly-Guillou ML, Vieu JF. Epidemiology of nosocomial infections due to Acinetobacter calcoaceticus. J Hosp Infect 1987; 10: 105-13.
• 4. Bouvet PJM, Grimont PAD. Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov. and Acinetobacter junii sp. nov. and emended descriptions of Acinetobacter calcoaceticus Acinetobacter Iwoffii. Int J Syst Bacteriol 1986; 36: 228-40.
• 5. Clegg S, Gerlach GF. Enterobacterial fimbriae. J Bacteriol 1987; 169: 934-38.
• 6. Davis ChP, Huston CW, Fader RC i inni. Immunoglobulin A and secretory immunoglobulin A antibody to purified type 1 Klebsiella pneumoniae pili in human colostrum. Infect Immun 1982; 38: 496-501.
• 7. Fleischer M, Przondo-Mordarska A. Występowanie gatunków rodzaju Acinetobacter w mate¬riale od chorych i w środowisku szpitalnym. Med Dośw Mikrobiol 1993; 45: 213-17.
• 8. Hanne LF, Finkelstein RA. Characterization and distribution of the hemagglutinius produced by Vibro cholerae. Infect Immun 1982; 36: 209-14.
• 9. Henrichsen J, Blom J. Correlation between twitching motility and possession of polar fimbriae in Acinetobacter calcoaceticus. Acta Pathol Microbiol Scand (B) 1975; 83: 103-15.
• 10. Hornick DB, Allen BL, Horn MA i inni. Fimbrial types among respiratory isolates belonging to the family Enterobacteriacea. J Clin Microbiol 1991; 29: 1795-1800.
• 11. Jann K, Hoschutzky H. Nature and organization of adhesins. Current Topies in Microbiology and Immunology. 1990; 151: 55-69.
• 12. Jones GW, Freter R. Adhesive properties of Vibro cholerae: nature of the interaction with isolated rabbit brush border membranes and human erythrocytes. Infect Immun 1976; 14: 240-45.
• 13. Kleiman R. Bacteria-immune system interactions II. Adherence of Acinetobacter calcoaceticus strains to human lymphocytes. Immunol Commun 1983; 12: 473-80.
• 14. Koljalg S, Vuopio-Varkila J, Lyytikainen O i inni. Cell surface properties of Acinetobacter baumannii. APMIS. 1996, 104: 659-66.
• 15. Mobley HLT, Chippendale GR, Tenney JH i inni. MR/K hemagglutination of Providencia stuartii correlates with persistence 30. in: catheter-associated bacteriuria. J Infect Dis 1988; 157: 264-71.
• 16. Murray PA, Kern DG, Winkler JR. Identification of a galactose-binding lectin on Fusobacte¬rium nuceatum FN-2. Infect Immun 1988; 56: 1314-19.
• 17. Ofek I, Sharon N. Lectinophagocytosis: a molecular mechanism of recognition between cell surface sugars and lectins in the phagocytosis of bacteria. Infect Immun 1988; 56: 539-47.
• 18. Ofek I, Sharon N. Adhesions as lectins: specificity and role in infection. Curr Topies Microbiol Immun 1990; 151, 91-113.
• 19. Przondo-Hessek H, Pulverer G. Hemagglutinins of Klebsiella pneumoniae and Klebsiella oxytoca. Zbl Bakt Hyg I Abt Orig A, 1983; 255: 472-78.
• 20. Rodriguez-Ortega M, Ofek I, Sharon N. Membrane glycoproteins of human polymorphonuc¬lear leukocytes that act as receptors for mannose-specific Escherichia coli. Infect Immun 1987; 55: 968-73.
• 21. Rosenberg M, Perry A, Bayer EA i inni. Adherence of Acinetobacter calcoaceticus RAG-1 to human epithelial cells and hexadecane. Infect Immun 1981; 33: 29-33.
• 22. Sharon N, Lis H. Glycoproteins in: Neurath H, Hill RL (eds.) The proteins. 3-rd edn. Academic. New York, 1982; 5: 1-144.
• 23. Tarrkanen AM, Allen BL, Westerlund B i inni. Type V collagen as the target for type-3 fimbriae, enterobacterial adherence organells. Mol Microbiol 1990; 4: 1353-61.
• 24. Vaisanen-Rhen V, Korhonen TK, Jokinen M i inni. Blood group M specific hemagglutynin in pyelonephritogenic Escherichia coli. Lancet, 1982; i: 1192