Succinate dehydrogenase and acid phosphatase activity in Phaseolus lunatus testa

• Autorzy: Aniszewski T
• Języki publikacji: EN

Abstrakty

EN

Succinate dehydrogenase (SDH) and acid phosphatase (AcP) activity in Phaseolus lunatus seed testa are demonstrated in enzyme tests, and uptake and transport of vital and indicator dyes such as methylene blue, Congo red and tetrazolium in the seed testa are examined by light and transmission electron microscopy. SDH activity was observed in the vascular bundles (endotesta) and in some cells in endo- and mesotesta. AcP activity was located near cell walls in both meso- and endotesta. In the vascular bundles there was very little AcP activity. Vital and indicator dyes were conducted from the exotesta (hilum) to endotesta. Vesicle mobilization was observed in the mesotesta. Strong enzyme activity in the meso- and endotesta and vesicle mobilization in the mesotesta suggest the potential active role of testa strata in imbibition and the initial nutritional stage of germination.

Słowa kluczowe

EN

succinate dehydrogenase; acid phosphatase; testa; seed testa; Congo red; botany; methylene blue; Phaseolus lunatus; tetrazolium

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2006
• Tom: 48
• Numer: 2
• Opis fizyczny: p.59-65,fig.,ref.

Twórcy

autor

Aniszewski T

• University of Joensuu, P.O.Box 111, 80101 Joensuu, Finland

Bibliografia

• Aniszewski T. 2005. SDH and AcP activities In the seed testa strata of Phaseolus lunatus. In: Spalding E, Thomashow M, Long SP, Balley-Serres J, Reddy ASN, Jones AM, and Kunkel B [eds.]. Plant biology. 198-199. American Society of Plant Biologists, Seattle, Washington.
• Aniszewski T, Karttunen A-L, and Hyvarinen H. 2006. Structure of Phaseolus lunatus testa at its central point. Acta Biologica Cracoviensia Series Botanica 48/1: 69-76.
• Bailey LH. 1963. The standard cyclopedia of horticulture, vol 3. 21st edition. The Mcmillan Company. New York.
• Baudoin JP. 1988. Genetic resources, domestication and evolution of Lima bean (Phaseolus lunatus). In: Gepts P. [ed.], Genetic resources of phaseolus beans, 393-407. Kluwer Academic Publishers, Dordrecht.
• Bi IZ, Maquet A, and Baudoin JP. 2003. Population genetic structure of wild Phaseolus lunatus (Fabaceae) with special reference to population sizes. American Journal of Botany 90: 897-904.
• Bressani R, and Elias LG. 1980. Nutritional value of legume crops for humans and animals. In: Summerfield RJ, and Bunting AH [eds.], Advances in legume science, 135-155. Proceedings of International Legume Conference, Kew.
• Burstone M. 1961. Histochemical demonstration of phosphatases in frozen sections with AS-phosphates. Journal of Histochemical Cytochemistry 9: 146-153.
• Campbell NA, Reece JB, and Mitchell LG. 1999. Biology. 5th Edition. Addison-Wesley Longmen Inc, Menlo Park, California.
• Čirković TD, Gavrović-Jankulović MDJ, Bukilica MN, Mandić L, Petrović SZ, and Jankov RM. 2002. Isolation and partial characterization of an acid phosphatase from Artemisia vulgaris pollen extract. Journal of Serbian Chemical Society 67: 567-572.
• Duke SH, and Kakefuda G. 1981. Role of the testa in preventing cellular rupture during imbibition of legume seed. Plant Physiology 67: 449-456.
• Duke SH, Kakefuda G, and Harvey TM. 1983. Differential leakage of intracellular substances from imbibing soybean seeds. Plant Physiology 72: 919-924.
• Fahn A. 1974. Plant anatomy. Pergamon Press, Oxford.
• Ferte N, Moustacs A-M, Nari J, Teisserre M, Borel M, Thiebart I, and Noat G. 1993. Characterization of kinetic properties of a soya-bean cell-wall phosphatase. European Journal of Biochemistry 211: 297-304.
• Fineran BA. 1997. Cyto and histochemical demonstration of lignins in plant cell walls: an evaluation of the chlorine water ethanolamine silver nitrate method of Coppick and Fowler. Protoplasma 198: 186-201.
• Freeman S. 2002. Biological science. Prentice-Hall, New York.
• Gueneron M, Timmers ACJ, Boucher C, and Arlat M. 2000. Two novel proteins, PopB, which has functional nuclear localization signals, and PopC, which has a large leucine-rich repeat domain, are secreted through the Hrp-secretion apparatus of Ralstonia solanecearum. Molecular Microbiology 36: 261-277.
• Hahalis D, Cochrane MP, and Smith ML. 1996. Water penetration sites in the testa of soybeans (Glycine max L Merril) during seed imbibition. Science Legumes 3: 218-226.
• Hare PD, Cress WA, and Van Staden J. 2003. A regulatory role for proline metabolism in stimulating Arabidopsis thaliana seed germination. Plant Growth Regulation 39: 41-50.
• Karcz J, Ksiazczyk T, and Maluszynska J. 2005. Seed coat patterns in rapid cycling Brassica forms. Acta Biologica Cracoviensia Series Botanica 47: 159-165.
• Kleinsmith LJ, and Rish VM. 1995. Principles of cell and molecular biology. Harper Collins, New York.
• Legesse N, and Powell AA. 1992. Comparison of water uptake and imbibition damage in eleven cowpea cultivars. Seed Science and Technology 20: 173-180.
• Lersten NR. 1979. A distinctive seed coat pattern in the Viceaceae (Papilionoideae, Leguminosae). Proceedings of Iowa Academy of Science 86: 102-104.
• Lioi L, and Galasso I. 2002. Oligonucleotide DNA fingerprinting revealing polymorphism in Phaseolus lunatus L. Genetic Resources and Crop Evolution 49: 53-58.
• Miao ZH, Fortuna JA, and Gallagher J. 2001. Anatomical structure and nutritive value of lupin seed coats. Australian Journal of Agricultural Research 52: 985-993.
• Newell CA, and Hymowitz T. 1978. Seed-coat variation in Glycine (Leguminosae) by SEM. Brittonia 30: 76-88.
• Nari J, Noat G, and Rilcard J. 1991. Pectine methylesterase, metal ions and plant cell-wall extension - hydrolysis of pectin by plant cell-wall pectin methylesterase. Biochemical Journal 279: 343-350.
• Oritani T, Fukuhara N, Okajima T, Kitamura F, and Ohsaka T. 2004. Electrochemical and spectroscopic studies on electron-transfer reaction between novel water-soluble tetrazolium salts and a superoxide ion. Inorganica Chimica Acta 357: 436-442.
• Pearse AGE. 1972. Histochemistry, theoretical and applied, vol. 2. 3rd ed. Churchill-Livingstone, Edinburg and London.
• Powell AA. 1990. The importance of genetically determined seed coat characteristics to seed quality in grain legumes. Annals of Botany 63: 169-175.
• Powell AA, and Matthews S. 1978. The damaging effect of water on dry pea embryos during imbibition. Journal of Experimental Botany 29: 1215-1229.
• Powell AA, Oliveira MDA, and Matthews S. 1986. The role of imbibition damage in determining the vigour of white and coloured seed coats of dwarf French beans (Phaseolus vulgaris). Journal of Experimental Botany 57: 716-722.
• Rudrapal D, Okubo H, Uemoto S, and Fujieda K. 1992. Comparison of the anatomy and physiology of seeds of 2 varieties of vinged bean (Psophocarpus tetragonolobus). Scientia Horticulturae 51: 13-24.
• Serva Electrophoresis GMBH. 2004. Enzymology. Tetrazolium Salts. Highly sensitive colour indicators of enzymatic reactions. Serva on line. Heidelberg. /http:www.serva.de/
• Trillo TA, and Carro AJM.1993. Germination, sead-coat structure and protein-patterns of seeds from Adenocarpus decorticans and Astragalus granatiensis growing at different altitudes. Seed Science Technology 21: 317-326.
• Tully RE, Musgrave ME, and Leopold AC. 1981. The seed coat as a control of imbibition chilling injury. Crop Science 21: 312-317.
• Vargas EM, Castro E, Macaya G, and Rocha OJ. 2003 Variation in fruit and seed size from 38 wild populations of Phaseolus lunatus (Fabaceae) from Central Valley, Costa Rica. Revista de Biologia Tropical 51: 707-724.
• Verbelen JP, and Stickens D.1995. In vivo determination of fibril orientation in plant-cell walls with polarization CSLM. Journal of Microscopy - Oxford 177: 1-6.
• Werker E. 1997. Seed anatomy. In: Carlquist S. Cutler DE, Fink S, Ozenda P, Roth I, and Ziegler [eds], Encyclopedia of plant anatomy, vol. 10, part 3, 1-424. Gebrüder Borntraeger, Berlin, Stuttgart.
• Windsor JB, Symonds VV, Mendenhall J, and Lloyd AM. 2000. Arabidopsis seed coat development: morphological differentiation on the outer integument. Plant Journal 22: 483-493.