Techniki molekularne stosowane w identyfikacji gatunkow Toxocara

• Autorzy: Fogt R

Warianty tytułu

EN

Molecular techniques applied in species identification of Toxocara

• Języki publikacji: PL

Abstrakty

EN

Toxocarosis is still an important and actual problem in human medicine. It can manifest as visceral (VLM), ocular (OLM) or covert (CT) larva migrans syndroms. Complicated life cycle of Toxocara, lack of easy and practical methods of species differentiation of the adult nematode and embarrassing in recognition of the infection in definitive hosts create difficulties in fighting with the infection. Although studies on human toxocarosis have been continued for over 50 years there is no conclusive answer, which of species - T. canis or T. cati constitutes a greater risk of transmission of the nematode to man. Neither blood serological examinations nor microscopic observations of the morphological features of the nematode give the satisfied answer on the question. Since the 90-ths molecular methods were developed for species identification and became useful tools being widely applied in parasitological diagnosis. This paper cover the survey of methods of DNA analyses used for identification of Toxocara species. The review may be helpful for researchers focused on Toxocara and toxocarosis as well as on detection of new species. The following techniques are described: PCR (Polymerase Chain Reaction), RFLP (Restriction Fragment Length Polymorphism), RAPD (Random Amplified Polymorphic DNA) and SSCP (Single Strand Conformation Polymorphism).

Słowa kluczowe

PL

identyfikacja gatunkowa; Toxocara canis; Toxocara cati; polimorfizm molekularny; lancuchowa reakcja polimerazy; nicienie; pasozyty; losowa amplifikacja DNA; Toxocara

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 2006
• Tom: 52
• Numer: 1
• Opis fizyczny: s.31-35,bibliogr.

Twórcy

autor

Fogt R

• Akademia Wychowania Fizycznego, ul.Krolowej Jadwigi 27/39, 61-871 Poznan

Bibliografia

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