EN
Nine strains of lactic acid bacteria isolated from raw pork and beef were the subject of the study. The antagonistic activity of the tested strains was determined against pathogenic bacteria: Staphylococcus aureus (MRSA and MSSA), enteropathogenic E. coli and Listeria monocytogenes. A modified Spelhaugh and Harlander method was used. The research was carried at anaerobic conditions and with the solid medium MRS-0.2. The lactic acid bacteria strains, which showed the largest antibacterial activity on the solid medium, were used for research in MRS-0.2 broth and minced beef. Pediococcus pentosaceus W24, Lactobacillus sp. W27, Lactobacillus brevis F4 strains showed the largest antibacterial activity against enteropathogenic E. coli strains on the solid medium. In MRS-0.2 broth in the presence of Lactobacillus sp. W27 (ltFCFU/ml) the growth of E. coli 6B0124:K72 was completely inhibited after five days of incubation. The number of Listeria monocytogenes cells in the presence of Pediococcus pentosaceus F50 (106CFU/ml) was lower than that of the control sample during the first two days. Incubation during the next four days resulted in an increase of the number of Listeria monocytogenes cells (6xl07CFU/ml) but the number of cells did not exceed the number of cells in the control sample (109CFU/ml). The antagonistic activity of these strains under investigation in minced beef was restricted. The growth of total of MRSA strains and majority of MSSA strains was inhibited by Pediococcus pentosaceus F50 and Lactobacillus brevis F14 strains on the solid medium MRS-0.2. In MRS-0.2 broth in the presence of Pediococcus pentosaceus F50 (106CFL/ml) the growth of MRSA 3 strain was completely inhibited after four days of incubation.