Odpowiedz immunologiczna kawki [Corvus monedula] przeciwko antygenom nicienia Capillaria resecta - technika western blotting

• Autorzy: Fronska-Popiel A , Okulewicz A. , Perec A.
• Języki publikacji: PL

Abstrakty

EN

Biotinylated surface and somatic protein extracts of the nematode Capillaria resecta were analysed by SDS-PAGE followed by Western blotting in order to examine their antigenic character. The antigens were probing with serum of C. resecta naturally infected birds (jackdaws). Surface and somatic antigens were recognised by serum IgG, IgA and IgM antibodies. The most intense reactivity of sera was evident with TBS-soluble surface proteins. The present paper has shown that surface proteins of C. resecta are more immunogenic than somatic and they are able to elicit stronger immune response in the bird's body.

Słowa kluczowe

PL

odpowiedz immunologiczna; pasozyty zwierzat; kawka; Capillaria resecta; przeciwciala; metoda western blot; parazytologia; nicienie; Corvus monedula

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 2001
• Tom: 47
• Numer: 4
• Opis fizyczny: s.693-698,bibliogr.

Twórcy

autor

Fronska-Popiel A

• Uniwersytet Wroclawski, ul.Przybyszewskiego 63, 51-148 Wroclaw

autor

Okulewicz A.

autor

Perec A.

Bibliografia

• ASHMAN K., MATHER J., WILTSHIRE C., JACOBS H.J., MEEUSEN E.1995. Isolation of a larval surface glucoprotein from Haemonchus contortus and its possible role in evading host immunity. Molecular Biochem. Parasitol. 70: 175-179.
• BARUŠ V., SERGEJEVA T.P. 1990. Capillariids parasitic in birds in the Palearctic region (3). Genus Baruscapillaria. Acta Scientarum Naturalium, Brno, 24 (10): 6-9.
• BLAXTER M. L., PAGE A. P., RUDIN W., MAIZELS R. M. 1992. Nematode surface coats: Actively evading immunity. Parasitology Today 8: 243-247.
• CANALS A., GASBARRE L.C. 1990. Ostertagia ostertagi isolation and patrial characterization of somatic and metabolic antigens. Int. J. Parasitol. 19: 57-61.
• DE GRAAF D. C., BERGHEN P., MOENS L., DE MAREZ T. M., RAES S., BLAXTER M. L., VERCRUYSSE J. 1996. Isolation, characterization and immunolocalization of a globinlike atigen from Osterlagia ostertagi adults. Parasitology 113: 63-69.
• DZIUBEK Z. (red.) 1996. Mechanizmy immunologiczne w zakażeniach pasożytniczych. PZWL. Warszawa.
• GOLIŃSKA Z. 1994. Przeciwciała powstąjące pod wpływem stymulacji antygenami pasożytów. Wiad. Parazylol. 30 (1): 73-83.
• KETIH K. A., DUNCAN M.C., MURRAY M., BAIRDENS K., TAIT A. 1990. Stage-specific cuticular proteins of Ostertagia circumcincta and Ostertagia ostertagi. Int. J. Parasitol. 20 (8): 1037-1045.
• LUSTIGMAN S., HUIMA T., BROTMAN B., MILLER K., PRINCE A. 1990. Onchocerca volvulus: Biochemical and morfological characteristics of the third- and fourth-stage larvae. Exp. Parasitol. 71 : 489-495.
• LUSTIGMAN S. 1993. Molting enzymes and new targets for chemotherapy of Onchocerca volvulus. Parasitology Today 9: 294-297.
• MAIZELS R. M., MEGHJI M., OGILVIE B. M. 1983. Restricted sets of parasite antigens from the surface of different sexes of the nematode parasite Nippostrongylus brasiliensis. Immunology 48: 107-121.
• SELKRIK M. E., NIELSEN L., KELLY C., PARTONO F., SAYERS G., MAIZELS R. M. 1989. Identification, synthesis and immunogenicity of cuticular collagens from the filarial nematodes Burgia malayi and Burgia pafangi. Molecular Biochem. Parasitol. 37: 73-86.
• TOWBIN H., STACKLIN T., GORDON J. 1979. Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76: 4350-4354.
• WĘDRYCHOWICZ H., BAIRDEN K., TAIT A., HOLMES P. H. 1992. Immune response of sheep to surface antigens of infective larvae of Ostertagia circumcincta. Parasite Immunology 14: 249-266.
• WĘDRYCHOWICZ H. 1994. Ostertagia circumcincta: surface and cuticular proteins and glycoproteins isolated by biotin-streptavidin affinity chromatography. Acta Parasitol. 39(1): 46-52.
• WĘDRYCHOWICZ H., HOLMES P. H., TAIT A. 1994. Surface antigens of exheathed infective larvae of Ostertagia circumcincta. Acta Parastol. 39(3): 162-169.
• WĘDRYCHOWICZ H., TAIT A., BAIRDEN K., DUNLOP E., HOLMES P. H. 1995. Immune response of lambs to vaccination with Ostertagia circumcincta surface antigens eliciting bile antibody responses. Int. J. Parasitol. 25: 1111-1121.
• WĘDRYCHOWICZ H. 1997. Antygeny powierzchniowe nicieni i ich rola w układzie pasożyt-żywiciel. Med. Wet. 53(10): 566-569.
• WU Z., NAGANO I., TAKAHASHI Y. 1999. A panel of antigens of muscle larvae of Jrichinella spiralis and T pseudospiralis as revealed by two-dimensional Western blot and immunoelectron microscopy. Parasitology 118: 615-622.