Wykrywanie Pasteurella multocida i Bordetella bronchiseptica - czynnikow etiologicznych zakaznego zanikowego zapalenia nosa swin przy pomocy testow PCR

• Autorzy: Markowska-Daniel I , Stepniewska K. , Pejsak Z.

Warianty tytułu

EN

Detection of Pasteurella multocida and Bordetella bronchiseptica - etiological agents of atrophic rhinitis, by means of PCR tests

• Języki publikacji: PL

Abstrakty

EN

Dermonecrotoxigenic (DNT) strains of Pasteurella multocida (Pm) and Bordetella bronchiseptica (Bbr), etiological agents of atrophic rhinitis (AR) of swine, are usually detected by conventional microbiological methods. Currently the main tasks in the diagnosis of infectious diseases is to detect the specific genes of pathogens directly from clinical samples within the shortest possible period of time by using sufficiently specific and sensitive methods. In this study two PCR tests were developed for the detection of genes encoding DNT in Pm and Bbr directly in nasal swabs. They were used for examining 481 nasal swabs collected from pigs raised in 56 farms, suspected of AR or displaying clinical symptoms of PAR. Additionally, the usefulness of PCR in the routine diagnosis of AR was compared with standard microbiological and serological methods. DNT+ Pm were directly evidenced in 74 samples (15.4%) from 27 tested herds (48.2%) while DNT+ Bbr were detected in 196 swabs (40.7%) from 41 farms (73.2%). Using the standard microbiological examination, the presence of bacterial cultures of the morphology typical of Pm was observed in 114 tested samples (23.7%) and those typical of Bbr, in 156 samples (32.45%). In the PCR test performed to confirm the presence of the DNT- -encoding gene in Pm isolates, positive results were obtained in 26 samples (22.8%), while among isolated Bbr strains, 95 out of 156 (60.1%) possessed the gene encoding DNT. In the ELISA test, 22 out of 26 isolates (84.6%) were DNT+. In the case of DNT- isolates of PM, the results of ELISA were in full agreement with PCR results. In total, PAR was detected in 27 farms (48.2%), NPAR in 21 farms (37.5%), and 8 farms (14.8%) were diagnosed as free from AR since no positive results were obtained by either method. Summarizing, the results of serological, bacteriological and molecular examinations clearly demonstrated the usefulness of PCR in the routine diagnosis of AR directly from clinical material.

Słowa kluczowe

PL

trzoda chlewna; choroby zwierzat; zakazne zanikowe zapalenie nosa; progresywne zakazne zanikowe zapalenie nosa; nieprogresywne zakazne zanikowe zapalenie nosa; czynniki chorobotworcze; Pasteurella multocida; Bordetella bronchiseptica; wykrywanie; badania bakteriologiczne; techniki biologii molekularnej; lancuchowa reakcja polimerazy; test PCR

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2010
• Tom: 66
• Numer: 01
• Opis fizyczny: s.45-50,fot.,tab.,bibliogr.

Twórcy

autor

Markowska-Daniel I

• Panstwowy Instytut Weterynaryjny - Panstwowy Instytut Badawczy, Al.Partyzantow 57, 24-100 Pulawy

autor

Stepniewska K.

autor

Pejsak Z.

Bibliografia

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