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2004 | 09 | 4B |

Tytuł artykułu

Identifying leaf rust resistance genes and mapping gene Lr37 on the microsatellite map of wheat

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Based on seedling resistance tests, five resistance genes (Lr10, Lr3, Lr13, Lr14a and Lr37) against leaf rust (Puccinia triticina) were identified in 16 cultivars of European winter wheat. STS and SCAR markers were used to verify the presence of the resistance genes Lr37 and Lr10 against leaf rust in cultivars, near-isogenic lines and segregating populations. The Lr37 gene is present in a small translocation from Triticum ventricosum Ces. (Aegilops ventricosa Tausch) and is tightly linked with resistance genes Yr17 and Sr38. The Lr37 gene was identified in the cultivars Kris, Clever, Slade, Apache, Caphorn, Lorraine, Balthasar, Renan and confirmed by two PCR markers. The F3 progenies of the crosses Kris (Lr37) x Nutka (Lr37 not present) were used for map construction. Two STS/SCAR markers specific for Lr37 were mapped in relation to nine polymorphic microsatellites on chromosome 2AS. The microsatellite marker Xgwm1176 mapped relatively close to the STS and SCAR markers for Lr37 with a linkage distance of 4.1 cM.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

09

Numer

4B

Opis fizyczny

p.869-878,fig.,ref.

Twórcy

autor
  • Polish Academy of Sicneces, Strzeszynska 34, 60-479 Poznan, Poland
autor
autor
autor
autor

Bibliografia

  • 1. Park, R.F., Goyeau, H., Felsenstein, F.G., Bartoš, P. and Zeller, F.J. Regional phenotypic diversity of Puccinia triticina and wheat host resistance in western Europe, 1995. Euphytica 122 (2001) 113-127.
  • 2. Winzeler, M., Mesterhazy, A., Park, R.F., Bartoš, P. and Csosz, M. Resistance of European winter wheat germplasm to leaf rust. Agronomie 20 (2000) 783-792.
  • 3. McIntosh, R.A. Breeding wheat for resistance to biotic stresses. Euphytica (1998) 19-34.
  • 4. McIntosh, R.A., Appels, R., Devos, K.M., Dubcovsky, J., Rogers, W.J. and Yamazaki, Y. Catalogue of gene symbols for wheat. Proc. 10th Intern. Wheat Genet. Symp. Paestum Italy, 2003.
  • 5. Autrique, E., Singh, R.P., Tanksley, S.D. and Sorrells, M.E. Molecular markers for four leaf rust resistance genes introgressed into wheat from wild relatives. Genome 38 (1995) 75-83.
  • 6. Friebe, B., Jiang, J., Raupp, W.J., McIntosh, R.A. and Gill, B.S. Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91 (1996) 59-87.
  • 7. Singh, D., Park, R.F. and McIntosh, R.A. Postulation of leaf (brown) rust resistance genes in 70 wheat cultivars grown in the United Kingdom. Euphytica 120 (2001) 205-218.
  • 8. Ambrozkowá, M., Dedryver, F., Dumalasova, V., Hanzalowa, A. and Bartoš, P. Determination of the cluster of wheat rust resistance genes Yr17, Lr37 and Sr38 by a molecular marker. Plant Prot. Sci. 38 (2002) 41-45.
  • 9. Chełkowski, J., Golka, L. and Stępień, Ł. Application of STS markers for leaf rust resistance genes in near-isogenic lines of spring wheat cv. Thatcher. J. Appl. Genet. 44 (2003) 323-338.
  • 10. Stępień, Ł., Golka, L. and Chełkowski, J. Leaf rust resistance genes of wheat: identification in cultivars and resistance sources. J. Appl. Genet. 44 (2003) 193-149.
  • 11. Stakman, E.C. and Stewart, D.M. Identification of physiology races of Puccinia graminis var. tritici. U.S. Agric. Res. Serv. 617 (1962) 1-53.
  • 12. Röder, M.S., Korzun, V., Wendehake, K., Plaschke, J., Tixier, M.H., Leroy, P. and Ganal, M.W. A microsatellite map of wheat. Genetics 149 (1998) 2007-2023.
  • 13. Lander, E.S., Green, P., Abrahamson, J., Barlow, A., Daly, M.J., Lincoln, S.E. and Newburg, L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1 (1987) 174-181.
  • 14. Kosambi, D.D. The estimation of map distances from recombination values. Ann. Eugen. 12 (1994) 172-175.
  • 15. Koebner, R. and Summers, R. The impact of molecular markers on the wheat breeding paradigm. Cell. Mol. Biol. Lett. 7(2002) 695-702.
  • 16. Korzun, V. Use of molecular markers in cereal breeding. Cell. Mol. Biol. Lett. 7 (2002) 811-820.
  • 17. William, H.M., Crosby, M., Trethowan, N R., Ginkel, M., Mujeeb-Kazi, A., Pfeiffer, W., Khairallah, M. and Hoisington, D. Molecular marker service laboratory at CIMMYT: An interface between the laboratory and the field. Tenth Intern. Wheat Genet. Symp., Paestum, Italy, 2 (2003) 852-854.
  • 18. Brown-Guedira, G. and Singh, S. Disease resistance. Leaf rust. Lr37. (2003) http://maswheat.ucdavis.edu/protocols/lr39/index.htm.
  • 19. Chełkowski, J. and Stępień, Ł. Molecular markers for leaf rust resistance genes in wheat. J. Appl. Genet. 42 (2001) 117-126.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-bfaf35e3-05ab-4c66-8562-1d2d32d20d29
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