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2002 | 07 | 2B |

Tytuł artykułu

The use of RAPD and Semi-random markers to verify somatic hybrids between diploid lines of Solanum tuberosum L.

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Markers specific to diploid lines of cultivated potato were identified using the polymerase chain reaction (PCR), primed with arbitrary 10-mers generating RAPDs, or with semi-random primers targeting intron-exon semiconservative sequences of plant genes. One hundred and fifty RAPD primers and twelve semi-random primers were tested. Selected primers were subsequently used for verification of putative somatic hybrids.

Wydawca

-

Rocznik

Tom

07

Numer

2B

Opis fizyczny

p.671-676,fig.

Twórcy

  • Plant Breeding and Acclimatization Institute, Radzikow, 05-870 Blonie, Poland
autor

Bibliografia

  • 1. Baird, E., Cooper-Bland, S., Waugh, R., DeMaine, M. and Powell, W. Molecular characterization of inter- and intra-specific hybrids of potato using randomly amplified polymorphic DNA (RAPD) markers. Mol. Gen. Gent. 233 (1992) 469-475.
  • 2. Standler, M., Stelzer, T., Borisjuk, N., Zanke, C., Schilde-Rentscheler, L. and Hemleben, V. Distribution of novel and known repeated elements of Solanum and application for the identification of somatic hybrids among Solanum species. Theor. Appl. Gent. 92 (1995) 1271-1278.
  • 3. Oberwalder, B., Ruoß, B., Schilde-Rentschler, L., Hemleben, V. and Ninnemann, H. Asymmetric protoplast fusion between wild and cultivated species of potato (Solanum ssp.) - detection asymmetric hybrids and genome elimination. Theor. Appl. Gent. 94 (1997) 1104-1112.
  • 4. Weining, S. and Langridge, P. Identification and mapping of polymorphisms in cereales based on the polymerase chain reaction. Teor. Appl. Gent. 82 (1991) 209-216.
  • 5. Rafalski, A., Gizińska, M. and Wiśniewska, I. PCR-based systems for evaluation of relationships among maize inbreds. Gent. Biot. and Breed. Maize and Sorghum. (Tsaftaris, A. S. Ed.), Royal Soc. Chem. Cambridge U. K. (1997) 106-111.
  • 6. Przetakiewicz, J., Nadolska-Orczyk, A. and Orczyk, W. Somatic hybridization of diploid genotypes of potato (Solanum tuberosum). IX Ogólnopolska Konf. Kul. In Vitro i Biot. Roślin. 10-13 wrzesień, Gdańsk (2000).
  • 7. Murray, A. A. and Thompson, W. F. Rapid isolation of high molecular weight plant DNA. Nucleic Acid Res. 8 (1980) 4321 - 4325.
  • 8. Williams, J. G. K., Kubelik, A. R., Livak, K. J.,Rafalski J. A. and Tingey, S. V. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Res. 18 (1990) 6531-6535.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-bebf6cb5-917a-4818-bfbb-3dba545c77f2
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