Proliferative processes within the equine corpus luteum may depend on paracrine progesterone actions

• Autorzy: Ferreira-Dias G. , Costa A.S. , Mateus L. , Korzekwa A. , Redmer D.A. , Skarzynski D.J.
• Języki publikacji: EN

Abstrakty

EN

Soon after ovulation, the corpus luteum (CL) starts secreting progesterone (P4), a hormone necessary for implantation. The aim of the study was to evaluate whether P4 exerts an autocrine/paracrine action on luteal angiogenic activity and P4, prostaglandin E2 (PGE2) and NO production in the mare. Corpora hemorrhagica (CH) and mid-luteal phase CL (MCL) were cultured with (i) no hormone (Control); (ii) P4; (iii) a P4 precursor - pregnenolone; or (iv) a P4 antagonist - onapristone [10-4M;10-5M; all steroids]. NO production decreased in MCL, with respect to CH, when treated with P4 [10-4M] and pregnenolone [10-5M]. PGE2 increased from CH to MCL, and showed a tendency to rise in pregnenolone treated luteal tissues (10-4M; p=0.06). In the CH, P4 decreased with pregnenolone [10-4M] compared to control, P4 [10-5M], onapristone [10-4M;10-5M] and pregnenolone [10-5M](p<0.05). In the MCL, pregnenolone [10-5M] decreased (p<0.05) and P4 tended to decrease (p=0.06) bovine aortic endothelial cell (BAEC) mitogenesis. Onapristone [10-4M] increased BAEC proliferation with respect to P4 (p=0.01). Since there was no direct effect of treatments on BAEC, these data suggest that long-lasting effects of P4 and its precursor may inhibit angiogenic factor(s) production by equine MCL, preparing for CL functional and structural regression.

Słowa kluczowe

EN

proliferative process; corpus luteum; paracrine progesterone action; implantation; prostaglandin E2; mare; nitric oxide; progesterone; prostaglandin; endocrine change; vascular change

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology. Supplement
• Rocznik: 2006
• Tom: 57
• Numer: 8
• Opis fizyczny: p.139-151,fig.,ref.

Twórcy

autor

Ferreira-Dias G.

• Faculdade de Medicina Veterinaria, Av. da Universidade Tecnica, 1300-477 Lisboa, Portugal

autor

Costa A.S.

autor

Mateus L.

autor

Korzekwa A.

autor

Redmer D.A.

autor

Skarzynski D.J.