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2006 | 09 | 4 |
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Conglutinin, CL-43 and CL-46 - three bovine collectins

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Języki publikacji
Conglutinin, collectin-43 (CL-43) and collectin-46 (CL-46) are serum proteins characteristic for Bovidae. They belong to collectins - family of oligomeric proteins composed of trimeric subunits containing collagen-like sequences joined to C-type lectin domains. The genes encoding conglutinin, CL-43 and CL-46 are located on the bovine chromosome 28, and phylogenetic analysis indicates their common origin - from the lung surfactant protein D gene. Northern blot or immunocytochemical analysis confirm biosynthesis of bovine collectins mainly in the liver (conglutinin, CL-43) and in the thymus (CL-46). The level of conglutinin in the serum of dairy cows depends on many factors such as breeding, the season of the year, the stage of the reproductive cycle and infection. The collectins are involved in the innate immune defense. They bind to microbial surface carbohydrates inducing aggregation and, thereby, impeding infectivity. On the other hand the destruction of pathogens occurs due to stimulation of effector cells. CL-43 as well as conglutinin, binds to the collectin receptor (ClqR) localized on many types of cells identified as a surface variant of calreticulin. Conglutinin and CL-43 show antiviral activities towards influenza A virus and rotaviruses. Conglutinin also displays protective activity against bacterial infections.
Opis fizyczny
  • Agricultural Academy, Akademicka 12, 20-033 Lublin, Poland
  • Andersen O, Sorensen AM, Svehag SE, Fenouillet E (1991) Conglutinin binds the HIV-1 envelope glycoprotein gp 160 and inhibits its interaction with cell membrane CD4. Scand J Immunol 33: 81-88.
  • Andersen O, Nielsen EH, Storgaard P, Hojrup P, Friis P, Leslie G, Svehag SE (1992a) Biochemical and ultrastruc- tural studies of the C-type lectin bovine conglutinin. J Struct Biol 109: 201-207.
  • Andersen O, Friis P, Holm Nielsen E, Vilsgaard K, Leslie RG, Svehag SE (1992b)Purification, subunit characteriz­ation and ultrastructure of three soluble bovine lectins: conglutinin, mannose-binding protein and the pentraxin serum amyloid P-component. Scand I Immunol 36: 131-141.
  • Araga S, Irie H, Takahashi K (1984) Conglutinin microtiter plate ELISA system for detecting circulating immune complexes. J Neuroimmunol 6: 161-168.
  • Arora M, Munoz E, Tenner AJ (2001) Identification of a site on mannan-binding lectin critical for enhancement of phagocytosis. J Biol Chem 16;276: 43087-43094.
  • Atochina EN, Beck JM, Preston AM, Haczku A, Tomer Y, Scanlon ST, Fusaro T, Casey J, Hawgood S, Gow AJ, Beers MF (2004) Enhanced lung injury and delayed clearance of Pneumocystis carinii in surfactant protein A-deficient mice: attenuation of cytokine responses and reactive oxygen-nitrogen species. Infect Immun 72: 6002-6011.
  • Bobak DA, Gaither TA, Frank MM, Tenner AJ (1987) Modulation of FcR function by complement: subcompo­nent Clq enhances the phagocytosis of IgG-opsonized targets by human monocytes and culture-derived macro­phages. J Immunol 15; 138: 1150-1156.
  • Coombs RRA, Coombs AM, Ingram DG (1961) The serol­ogy of conglutination and its relation to disease. Black- well Sci Publ, Oxford.
  • D’Amelio R, Brighouse G, Barnet M, Lambert PH (1981) Antigen-specific detection of soluble immune complexes in conglutinin-binding assays. Clin Exp Immunol 45: 283-289.
  • Eda S, Suzuki Y, Kase T, Kawai T, Ohtani K, Sakamoto T, Kurimura T, Wakamiya N (1996) Recombinant bovine conglutinin, lacking the N-terminal and collagenous do­mains, has less conglutination activity but is able to in­hibit haemagglutination by influenza A virus. Biochem J 316: 43-48.
  • Eggleton P, Lieu TS, Zappi EG, Sastry K, Coburn J, Zaner KS, Sontheimer RD, Capra JD, Ghebrehiwet B, Tauber AI (1994a) Calreticulin is released from activated neu­trophils and binds to Clq and mannan-binding protein. Clin Immunol Immunopathol 72: 405-409.
  • Eggleton P, Ghebrehiwet B, Coburn JP, Sastry KN, Zaner KS, Tauber AI (1994b) Characterization of the human neutrophil Clq receptor and functional effects of free ligand on activated neutrophils. Blood 84: 1640-1649.
  • Favoreel HW, Van de Walle GR, Nauwynck HJ, Pensaert MB (2003) Virus complement evasion strategies. J Gen Virol 84: 1-15.
  • Fischer PB, Ellermann-Eriksen S, Thiel S, Jensenius JC, Mogensen SC (1994) Mannan-binding protein and bov­ine conglutinin mediate enhancement of herpes simplex virus type 2 infection in mice. Scand J Immunol 39: 439-445.
  • Friis P, Svehag SE, Andersen O, Gahrn-Hansen B, Leslie RG (1991) Conglutinin exhibits a complement-depend­ent enhancement of the respiratory burst of phagocytes stimulated by E. coli. Immunology 74: 680-684.
  • Friis-Christiansen P, Thiel S, Svehag SE, Dessau R, Sven- dsen P, Andersen O, Laursen SB, Jensenius JC (1990) In vivo and in vitro antibacterial activity of conglutinin, a mammalian plasma lectin. Scand J Immunol 31: 453-460.
  • Gallagher DS Jr, Ryan AM, Liou LS, Sastry KN, Womack JE (1993) Somatic cell mapping of conglutinin (CGN1) to cattle syntenic group U29 and fluorescence in situ lo­calization to Chromosome 28. Mamm Genome 4: 716-719.
  • Ghebrehiwet B, Silvestri L, McDevitt C (1984) Identifica­tion of the Raji cell membrane-derived Clq inhibitor as a receptor for human Clq. Purification and im­munochemical characterization. J Exp Med 160: 1375-1389.
  • Ghiran I, Klickstein LB, Nicholson-Weller A (2003) Cal­reticulin is at the surface of circulating neutrophils and uses CD59 as an adaptor molecule. J Biol Chem 278: 21024-21031.
  • Gjerstorff M, Hansen S, Jensen B, Dueholm B, Horn P, Bendixen C, Holmskov U (2004) The genes encoding bovine SP-A, SP-D, MBL-A, conglutinin, CL-43 and CL-46 form a distinct collectin locus on Bos taurus chro­mosome 28 (BTA28) at position q.1.8-1.9. Anim Genet 35: 333-337.
  • Guan E, Robinson SL, Goodman EB, Tenner AJ (1994) Cell-surface protein identified on phagocytic cells modu­lates the Clq-mediated enhancement of phagocytosis. J Immunol 152: 4005-4016.
  • Hakansson K, Reid KB (2000) Collectin structure: a review. Protein Sci 9: 1607-1617.
  • Hansen S, Holmskov U (1998) Structural aspects of collec- tins and receptors for collectins. Immunobiology 199: 165-189.
  • Hansen S, Holm D, Moeller V, Vitved L, Bendixen C, Reid KB, Skjoedt K, Holmskov U (2002) CL-46, a novel col­lectin highly expressed in bovine thymus and liver. J Im­munol 169: 5726-5734.
  • Hansen S, Holm D, Moeller V, Vitved L, Bendixen C, Skjoedt K, Holmskov U (2003) Genomic and molecular characterization of CL-43 and its proximal promoter. Biochim Biophys Acta 1625: 1-10.
  • Hartley CA, Jackson DC, Anders EM (1992) Two distinct serum mannose-binding lectins function as beta inhibi­tors of influenza virus: identification of bovine serum beta inhibitor as conglutinin. J Virol 66: 4358-4363.
  • Hartshorn KL, Sastry K, Brown D, White MR, Okarma TB, Lee YM, Tauber AI (1993) Conglutinin acts as an op­sonin for influenza A viruses. J Immunol 151: 6265-6273.
  • Hartshorn K, Chang D, Rust K, White M, Heuser J, Crouch E (1996a) Interactions of recombinant human pulmon­ary surfactant protein D and SP-D multimers with influ­enza A. Am J Physiol 271: L753-762.
  • Hartshorn KL, Reid KB, White MR, Jensenius JC, Morris SM, Tauber AI, Crouch E (1996b) Neutrophil deactiva­tion by influenza A viruses: mechanisms of protection after viral opsonization with collectins and hemagglutina­tion-inhibiting antibodies. Blood 87: 3450-3461.
  • Hartshorn KL, White MR, Shepherd V, Reid K, Jensenius JC, Crouch EC (1997) Mechanisms of anti-influenza ac­tivity of surfactant proteins A and D: comparison with serum collectins. Am J Physiol 273: L1156-1166.
  • Hartshorn KL, Crouch E, White MR, Colamussi ML, Kak- kanatt A, Tauber B, Shepherd V, Sastry KN (1998) Pul­monary surfactant proteins A and D enhance neutrophil uptake of bacteria. Am J Physiol 274: L958-969.
  • Hartshorn KL, Sastry KN, Chang D, White MR, Crouch EC (2000) Enhanced anti-influenza activity of a surfactant protein D and serum conglutinin fusion protein. Am J Physiol Lung Cell Mol Physiol 278: L90-98.
  • Hartshorn KL, Holmskov U, Hansen S, Zhang P, Meschi J, Mogues T, White MR, Crouch EC (2002) Distinctive anti-influenza properties of recombinant collectin 43. Biochem J 366: 87-96.
  • Haurum JS, Thiel S, Haagsman HP, Laursen SB, Larsen B, Jensenius JC (1993) Studies on the carbohydrate-binding characteristics of human pulmonary surfactant-associated protein A and comparison with two other collectins: mannan-binding protein and conglutinin. Biochem J 293: 873-878.
  • Heinonen O, Syrjanen S, Soininen H, Talasniemi S, Kaski M, Mantyjarvi R, Syrjanen K, Riekkinen P Sr (1993) Cir­culating immune complexes in sera from patients with Alzheimer’s disease, multi-infarct dementia and Down’s syndrome. Neurosci Lett 149: 67-70.
  • Hirani S, Lambris JD, Muller-Eberhard HJ (1985) Localiz­ation of the conglutinin binding site on the third compo­nent of human complement. J Immunol. 134: 1105-1109.
  • Hirani S, Lambris JD, Muller-Eberhard HJ (1986) Struc­tural analysis of the asparagine-linked oligosaccharides of human complement component C3. Biochem J 233: 613-616.
  • HolmskovU, Haas H, Teisner B, Andersen O, Jensenius JC 1992) Calcium-dependent and calcium-independent sig­nals in the conglutinin-binding assay (KgBa) for immune complexes. Influence of anti-collagen-antibodies. J Im­munol Methods 148: 225-232.
  • Holmskov U, Teisner B, Pedersen NT, Laursen SB, Rasmus­sen HB, Jensenius JC (1992) Tissue localization of con- glutinin, a bovine C-type lectin. Immunology 76: 169-173.
  • Holmskov U, Teisner B, Willis AC, Reid KB, Jensenius JC 1993) Purification and characterization of a bovine serum lectin (CL-43) with structural homology to con­glutinin and SP-D and carbohydrate specificity similar to mannan-binding protein. J Biol Chem 1993 268: 10120-10125.
  • Holmskov U, Laursen SB, Malhotra R, Wiedemann H, Timpl R, Stuart GR, Tornoe I, Madsen PS, Reid KB, Jensenius JC (1995) Comparative study of the structural and functional properties of a bovine plasma C-type lec­tin, collectin-43, with other collectins. Biochem J 305: 889-896.
  • Holmskov U, Jensenius JC, Tornoe I, Lovendahl P (1998) The plasma levels of coglutinin are heritable in cattle and low levels predispose to infection. Immunology 93: 431-436.
  • Holmskov UL (2000) Collectins and collectin receptors in innate immunity. APMIS Suppl 100: 1-59.
  • Hoppe HJ, Reid KB (1994) Collectins-soluble proteins con­taining collagenous regions and lectin domains-and their roles in innate immunity. Protein Sci 3: 1143-1158.
  • Ingram DG (1959a) The conglutination phenomenon. XIII. In vivo interactions of conglutinin and experimental bac­terial infection. Immunology 2: 322-333.
  • Ingram DG (1959b) The conglutination phenomenon. XIV. The resistance enhancing effect of conglutinin and im- muno-conglutinin in experimental bacterial infections. Immunology 2: 334-345.
  • Ingram DG, Barnum DA (1965) Fluctuations in the level of conglutinin in bovine serum. Can Vet J 11: 162-169.
  • Ingram DG, Mitchell WR (1970) Conglutinin levels in dairy cattle: changes associated with parturition. Am J Vet Res 31: 487-492.
  • Ingram DG, Mitchell WR (1971a) Conglutinin levels in dairy cattle: seasonal fluctuations. Am J Vet Res 32: 23-28.
  • Ingram DG, Mitchell WR (1971b) Conglutinin level in dairy cattle: changes associated with disease. Am J Vet Res 32: 875-878.
  • Kakoma I, Kinyanjui M (1974) The effect of breed and age on the distribution of conglutinin and immunoconglutinin in normal cattle. Res Vet Sci 17: 122-124.
  • Kawasaki N, Yokota Y, Kawasaki T (1993) Differentiation of conglutination activity and sugar-binding activity of conglutinin after removal of NH2-terminal 54 amino acid residues by endogenous serine protease(s). Arch Bio­ chem Biophys 305: 533-540.
  • Hartshorn KL, Crouch E, White MR, Colamussi ML, Kak- kanatt A, Tauber B, Shepherd V, Sastry KN (1998) Pul­monary surfactant proteins A and D enhance neutrophil uptake of bacteria. Am J Physiol 274: L958-969.
  • Kishore U, Sontheimer RD, Sastry KN, Zaner KS, Zappi EG, Hughes GR, Khamashta MA, Strong P, Reid KB, Eggleton P (1997) Release of calreticulin from neu­trophils may alter Clq-mediated immune functions. Bio­chem J 322: 543-550.
  • Kishore U, Ghai R, Greenhough TJ, Shrive AK, Bonifati DM, Gadjeva MG, Waters P, Kojouharova MS, Chak- raborty T, Agrawal A (2004) Structural and functional anatomy of the globular domain of complement protein Clq. Immunol Lett 95: 113-128.
  • Koch A, Melbye M, Sorensen P, Homoe P, Madsen HO, Molbak K, Hansen CH, Andersen LH, Hahn GW, Gar- red P (2001) Acute respiratory tract infections and man­nose-binding lectin insufficiency during early childhood. JAMA 285: 1316-1321
  • Kolatkar AR, Weis WI (1996) Structural basis of galactose recognition by C-type animal lectins. Biol Chem 271: 6679-6685.
  • Krogh-Meibom T, Holmskov U, Lovendahl P, Nielsen NI, Ingvartsen KL (2004a) A time-resolved immunof- luorometric assay for quantification of the bovine collec­tin conglutinin. J Immunol Methods 286: 87-96.
  • Krogh-Meibom T, Holmskov U, Lovendahl P, Ingvartsen KL(2004b) A time-resolved immunofluorometric assay for quantification of collectin-43. J Immunol Methods 295: 161-167.
  • Lachmann PJ (1962) A comparison of some properties of bovine conglutinin with those of rabbit immuno-con- glutinin. Immunology 5: 687-705.
  • Lachmann PJ (1967) Conglutinin and immunoconglutinins. Adv Immunol 6: 479-527.
  • Laursen SB, Thiel S, Teisner B, Holmskov U, Wang Y, Sim RB, Jensenius JC (1994) Bovine conglutinin binds to an oligosaccharide determinant presented by iC3b, but not by C3, C3b or C3c. Immunology 81: 648-654.
  • Lee YM, Leiby KR, Allar J, Paris K, Lerch B, Okarma TB (1991a) Primary structure of bovine conglutinin, a mem­ber of the C-type animal lectin family. J Biol Chem 266: 2715-2723.
  • Lee RT, Ichikawa Y, Fay M, Drickamer K, Shao MC, Lee YC (1991b) Ligand-binding characteristics of rat serum-type mannose-binding protein (MBP-A). Homol­ogy of binding site architecture with mammalian and chicken hepatic lectins. J Biol Chem 266: 4810-4815.
  • Lim BL, Lu J, Reid KB (1993) Structural similarity between bovine conglutinin and bovine lung surfactant protein D and demonstration of liver as a site of synthesis of conglutinin. Immunology 78: 159-165.
  • Lim BL, Willis AC, Reid KB, Lu J, Laursen SB, Jensenius JC, Holmskov U (1994) Primary structure of bovine col- lectin-43 (CL-43). Comparison with conglutinin and lung surfactant protein-D. J Biol Chem 269: 11820-11824
  • Liou LS, Sastry R, Hartshorn KL, Lee YM, Okarma TB, Tauber AI, Sastry KN (1994) Bovine conglutinin (BC) mRNA expressed in liver: cloning and characterization of the BC cDNA reveals strong homology to surfactant pro- tein-D. Gene 141: 277-281.
  • Loveless RW, Holmskov U, Feizi T (1995) Collectin-43 is a serum lectin with a distinct pattern of carbohydrate recognition. Immunology 85: 651-659.
  • Lu J (1997) Collectins: collectors of microorganisms for the innate immune system. Bioessays 19: 509-518.
  • Lu J, Laursen SB, Thiel S, Jensenius JC, Reid KB (1993) The cDNA cloning of conglutinin and identification of liver as a primary site of synthesis of conglutinin in mem­bers of the Bovidae. Biochem J 292: 157-162.
  • Madan T, Reid KB, Singh M, Sarma PU, Kishore U (2005) Susceptibility of mice genetically deficient in the surfac­tant protein (SP)-A or SP-D gene to pulmonary hyper­sensitivity induced by antigens and allergens of Aspergil­lus fumigatus. J Immunol 174: 6943-6954.
  • Malhotra R, Sim RB (1989) Chemical and hydrodynamic characterization of the human leucocyte receptor for complement subcomponent Clq. Biochem J 262: 625-631.
  • Malhotra R, Thiel S, Reid KB, Sim RB (1990) Human leukocyte Clq receptor binds other soluble proteins with collagen domains. J Exp Med 172: 955-959.
  • Malhotra R, Haurum J, Thiel S, Sim RB (1992) Interaction of Clq receptor with lung surfactant protein A. Eur J Im­munol 22: 1437-1445.
  • Malhotra R, Laursen SB, Willis AC, Sim RB (1993) Localiz­ation of the receptor-binding site in the collectin family of proteins. Biochem J 293: 15-19.
  • Michalak M, Corbett EF, Mesaeli N, Nakamura K, Opas M (1999) Calreticulin: one protein, one gene, many func­tions. Biochem J 344: 281-292.
  • Mizuochi T, Loveless RW, Lawson AM, Chai W, Lachmann PJ, Childs RA, Thiel S, Feizi T (1989) A library of oligosaccharide probes (neoglycolipids) from N-glycosylated proteins reveals that conglutinin binds to certain complex-type as well as high mannose-type oligosaccharide chains. J Biol Chem 264: 13834-13839.
  • Nepomuceno RR, Tenner AJ (1998) ClqRP, the Clq recep­tor that enhances phagocytosis, is detected specifically in human cells of myeloid lineage, endothelial cells, and platelets. J Immunol 160: 1929-1935.
  • Ng KK, Drickamer K, Weis WI (1996) Structural analysis of monosaccharide recognition by rat liver mannose-binding protein. Biol Chem 271: 663-674.
  • Ogden CA, deCathelineau A, Hoffmann PR, Bratton D, Ghebrehiwet B, Fadok VA, Henson PM (2001) Clq and mannose binding lectin engagement of cell surface cal­reticulin and CD91 initiates macropinocytosis and uptake of apoptotic cells. J Exp Med 194: 781-795.
  • Ohtani K, Suzuki Y, Eda S, Kawai T, Kase T, Yamazaki H, Shimada T, Keshi H, Sakai Y, Fukuoh A, Sakamoto T, Wakamiya N (1999) Molecular cloning of a novel human collectin from liver (CL-L1). J Biol Chem 274: 13681-13689.
  • Ohtani K, Suzuki Y, Eda S, Kawai T, Kase T, Keshi H, Sakai Y, Fukuoh A, Sakamoto T, Itabe H, Suzutani T, Ogasawara M, Yoshida I, Wakamiya N (2001) The mem­brane-type collectin CL-P1 is a scavenger receptor on vascular endothelial cells. J Biol Chem 276: 44222-44228.
  • Peerschke El, Malhotra R, Ghebrehiwet B, Reid KB, Willis AC, Sim RB (1993) Isolation of a human endothelial cell Clq receptor (ClqR). J Leukoc Biol 53: 179-184.
  • Du Plessis JL (1985) The natural resistance of cattle to arti­ficial infection with Cowdria ruminantium: the role played by conglutinin. Onderstepoort J Vet Res 52: 273-277.
  • Purdy CW, Loan RW, Straus DC, Briggs RE, Frank GH (2000) Conglutinin and immunoconglutinin titers in stressed calves in a feedlot. Am J Vet Res 61: 1403-1409.
  • Ramig RF (2004) Pathogenesis of intestinal and systemic rotavirus infection. J Virol 78: 10213-10220.
  • Reading PC, Holmskov U, Anders EM (1998) Antiviral ac­tivity of bovine collectins against rotaviruses. J Gen Virol 79: 2255-2263.
  • Rose JE, Amerault TE, Roby TO, Martin WH (1978) Serum levels of conglutinin, complement, and im­munoconglutinin in cattle infected with Anaplasma mar­ginale. Am J Vet Res 39: 791-793.
  • Schelenz S, Malhotra R, Sim RB, Holmskov U, Bancroft GJ (1995) Binding of host collectins to the pathogenic yeast Cryptococcus neoformans: human surfactant protein D acts as an agglutinin for acapsular yeast cells. Infect Immun 63: 3360-3366.
  • Shi L, Takahashi K, Dundee J, Shahroor-Karni S, Thiel S, Jensenius JC, Gad F, Hamblin MR, Sastry KN, Ezekowitz RA (2004) Mannose-binding lectin-deficient mice are susceptible to infection with Staphylococcus aureus. J Exp Med 199: 1379-1390.
  • Stuart GR, Lynch NJ, Lu J, Geick A, Moffatt BE, Sim RB, Schwaeble WJ (1996) Localisation of the Clq binding site within Clq receptor/calreticulin. FEBS Lett 397: 245-249.
  • Stuart GR, Lynch NJ, Day AJ, Schwaeble WJ, Sim RB (1997) The Clq and collectin binding site within Clq receptor (cell surface calreticulin). Immunopharmacol- ogy 38: 73-80.
  • Suzuki Y, Yin Y, Makino M, Kurimura T, Wakamiya N (1993) Cloning and sequencing of a cDNA coding for bovine conglutinin. Biochem Biophys Res Commun 191: 335-342.
  • Tenner AJ, Robinson SL, Borchelt J, Wright JR (1989) Hu­man pulmonary surfactant protein (SP-A), a protein structurally homologous to Clq, can enhance FcR- and CRl-mediated phagocytosis. J Biol Chem 264: 13923-13928.
  • Tenner AJ, Robinson SL, Ezekowitz RA (1995) Mannose binding protein (MBP) enhances mononuclear phagocyte function via a receptor that contains the 126,000 M(r) component of the Clq receptor. Immunity 3: 485-493.
  • Thiel S, Frederiksen PD, Jensenius JC (2006) Clinical mani­festations of mannan-binding lectin deficiency. Mol Im­munol 43: 86-96.
  • van de Wetering JK, van Golde LM, Batenburg JJ (2004) Collectins: players of the innate immune system. Eur J Biochem 271: 1229-1249.
  • Vandivier RW, Ogden CA, Fadok VA, Hoffmann PR, Brown KK, Botto M, Walport MJ, Fisher JH, Henson PM, Greene KE (2002) Role of surfactant proteins A, D, and Clq in the clearance of apoptotic cells in vivo and in vitro: calreticulin and CD91 as a common collectin recep­tor complex. J Immunol 169: 3978-3986.
  • Wakamiya N, Okuno Y, Sasao F, Ueda S, Yoshimatsu K, Naiki M, Kurimura T (1992) Isolation and characteriz­ation of conglutinin as an influenza A virus inhibitor. Bio­chem Biophys Res Commun 187: 1270-1278.
  • Weis WI, Drickamer K, Hendrickson WA (1992) Structure of a C-type mannose-binding protein complexed with an oligosaccharide. Nature 360: 127-134.
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