Tracking chromatin states using controlled DNase I treatment and real-time PCR

• Autorzy: Martins R P , Platts A.E. , Krawetz S.A.
• Języki publikacji: EN

Abstrakty

EN

A novel approach to DNase I-sensitivity analysis was applied to examining genes of the spermatogenic pathway, reflective of the substantial morphological and genomic changes that occur during this program of differentiation. A new real-time PCR-based strategy that considers the nuances of response to nuclease treatment was used to assess the nuclease susceptibility through differentiation. Data analysis was automated with the K-Lab PCR algorithm, facilitating the rapid analysis of multiple samples while eliminating the subjectivity usually associated with Ct analyses. The utility of this assay and analytical paradigm as applied to nuclease-sensitivity mapping is presented.

Słowa kluczowe

EN

spermatogenesis; real-time polymerase chain reaction; differentiation; DNase I-sensitivity; analytical paradigm

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2007
• Tom: 12
• Numer: 4
• Opis fizyczny: p.545-555,fig.,ref.

Twórcy

autor

Martins R P

• Wayne State University School of Medicine, Detroit, Michigan, USA

autor

Platts A.E.

autor

Krawetz S.A.

Bibliografia

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