Herpesviruses as possible cofactors in HPV-16-related oncogenesis

• Autorzy: Szostek S. , Zawilinska B. , Kopec J. , Kosz-Vnenchak M.
• Języki publikacji: EN

Abstrakty

EN

 Cervical carcinogenesis is a complex problem with papillomavirus widely accepted as a causative agent. Integration of a human papillomavirus (HPV) of the high-risk type into the host cell genome is one of the major contributing factors to cervical malignant transformation. In this study, the correlation of CMV, EBV, HSV-1, HSV-2, HHV-6 and HHV-7 infections with the physical status of the HPV genome in cervical cancer and precancerous cervical lesions was investigated in sixty HPV-16-positive women. Cervical secretion samples were submitted to DNA extraction and analyzed by PCR. HPV-16 DNA was confirmed in genotyping with the reverse hybridization line probe assay. Multiplex PCR with specific primers for the E2/E6 genes was used to assess the viral integration status of HPV-16. Our results show that CMV DNA was more frequently present in samples with mixed forms of HPV-16 than in the episomal form (P < 0.025). Such a correlation was also observed in the case of EBV (P < 0.005). The presence of CMV resulted in a six-fold (OR 6.069; 95% CI 1.91-19.22; P = 0.002), while EBV caused a seven-fold (OR 7.11; 95% CI 1.70-29.67; P = 0.007) increase in the risk of the integrated or mixed HPV-16 genome occurrence. Our data suggest that coinfection with herpesviruses, especially CMV and EBV, may be involved in the integration of the HPV-16 genome and may contribute to the development of cervical cancer.

Słowa kluczowe

PL

microbiology; herpesvirus; human papillomavirus; papillomavirus; squamous intraepithelial lesion; cervical carcinoma; carcinogenesis; human herpesvirus; genome; polymerase chain reaction; cervical cancer

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2009
• Tom: 56
• Numer: 2
• Opis fizyczny: p.337-342,fig.,ref.

Twórcy

autor

Szostek S.

• Jagiellonian University Medical College, Chair of Microbiology, Czysta 18, 31-121 Krakow, Poland

autor

Zawilinska B.

autor

Kopec J.

autor

Kosz-Vnenchak M.

Bibliografia

• Broccolo F, Cassina G, Chiari S, Garcia-Parra R, Villa A, Leone BE, Brenna A, Locatelli G, Mangioni C, Cocuzza CE (2008) Frequency and clinical significance of human beta-herpesviruses in cervical samples from Italian women. J Med Virol 80: 147-153. 
• Cassinotti P, Mietz H, Siegl G (1996) Suitability and clinical application of a multiplex nested PCR assay for the diagnosis of herpes simplex virus infections. J Med Virol 50: 75-81. 
• Chan PKS, Peiris JSM, Yuen KY, Liang RHS, Lau YL, Chen FE, Lo SKF, Cheung CY, Chan TK, Ng MH (1997) Human herpesvirus-6 and human herpesvirus-7 infections in bone marrow transplant recipients. J Med Virol 53: 295-305. 
• Chan PK, Chan MY, Li WW, Chan DP, Cheung JL, Cheng AF (2001) Association of human beta-herpesviruses with the development of cervical cancer: bystanders or cofactors. J Clin Pathol 54: 48-53. 
• DiPaolo JA, Woodworth CD, Coutlée F, Zimonic DB, Bryant J, Kessous A (1998) Relationship of stable integration of herpes simplex virus-2 Bg/II N subfragment Xho2 to malignant transformation of human papillomavirus-immortalized cervical keratinocytes. Int J Cancer 76: 865-871. 
• Glaser SL, Hsu JL, Gulley ML (2004) Epstein-Barr virus and breast cancer: state of the evidence for viral carcinogenesis. Cancer Epidemiol Biomarkers Prev 13: 688-697. 
• Guzik K, Bzowska M, Dobrucki J, Pryjma J (1999) Heat-shocked monocytes are resistant to Staphylococcus aureus-induced apoptotic DNA fragmentation due expression of HSP 72. Infect Immun 67: 4216-4222. 
• Heggie AD, Wentz WB, Reagan JW, Anthony DD (1986) Roles of cytomegalovirus and Chlamydia trachomatis in the induction of cervical neoplasia in the mouse. Cancer Res 46: 5211-5214. 
• Howley PM, Lowy DR (2007) Papillomaviruses. In Filds virology. Knipe DM, Howley PM, eds, pp 2299. Lippincott Williams & Wilkins, a Wolters Kluwer Business, Philadelphia.
• Huang LW, Chao SL, Lee BH (2008) Integration of human papillomavirus type-16 and type-18 is very early event in cervical carcinogenesis. J Clin Pathol 61: 627-631. 
• Kim NR, Lin Z, Kim KR, Cho HY, Kim I (2005) Epstein-Barr virus and p16INK4A methylation in squamous cell carcinoma and precancerous lesions of the cervix uteri. J Korean Med Sci 20: 636-642. 
• Kleter B, van Doorn LJ, Schrauwen L, Molijn A, Sastrowijoto S, ter Schegget J, Linderman J, ter Harmsel B, Burger M, Quint W (1999) Development and clinical evaluation of a highly sensitive PCR-reverse hybridization line probe assay for detection and identification of anogenital human papillomavirus. J Clin Microbiol 37: 2508-2517.  
• Kulmala S-MA, Syrjänen SM, Gyllensten UB, Shabalova IP, Petrovichev N, Tosi P, Syrjänen KJ, Johansson BC (2006) Early integration of high copy HPV16 detectable in women with normal and low grade cervical cytology and histology. J Clin Pathol 59: 513-517. 
• Lanham S, Herbert A, Basarab A, Watt P (2001) Detection of cervical infections in colposcopy clinic patients. J Clin Microbiol 39: 2946-2950. 
• Lattario F, Furtado YL, Fonseca R, Silveira FA, do Val IC, Almeida G, Carvalho MG (2008) Analysis of human papillomavirus and Epstein-Barr virus infection and aberrant death-associated protein kinase methylation in high-grade squamous intraepithelial lesions. Int J Gynecol Cancer 18: 785-789.  
• Matias KP (2004) Studies of the viral origins of some cancers lead to new prevention, treatment strategies. OncoLog 49: 1-4.
• Mitchell SM, Fox JD, Tedder RS, Gazzard BG, Lightman S (1994) Vitreous fluid sampling and viral genome detection for the diagnosis of viral retinitis in patients with AIDS. J Med Virol 43: 336-340. 
• Rawls WE, Tompkins WA, Figueroa ME, Melnick JL (1968) Herpesvirus type 2: association with carcinoma of the cervix. Science 161: 1255-1256. 
• Romano N, Romano FM, Viviano E, Vitale F, Villafrate MR, Perna AM, Bonura F, Guttadauro R (1996) Rare association of human herpesvirus 6 DNA with human papillomavirus DNA in cervical smears of women with normal and abnormal cytologies. J Clin Microbiol 34: 1589-1591. 
• Sasagawa T, Shimakage M, Nakamura M, Sakaike J, Ishikawa H, Inoue M (2000) Epstein-Barr virus (EBV) genes expression in cervical intraepithelial neoplasia and invasive cervical cancer: a comparative study with human papillomavirus (HPV) infection. Hum Pathol 31: 318-326. 
• Scheurer ME, Tortolero-Luna G, Adler-Storthz K (2005) Human papillomavirus infection: biology, epidemiology, and prevention. Int J Gynecol Cancer 15: 727-746. 
• Schmidt MT, Olejnik AK, Goździcka-Józefiak A (2005) The HPV16 E2 transcriptional regulator mode of action depends on the physical state of the viral genome. Acta Biochim Polon 52: 823-832. 
• Shen Y, Zhu H, Shenk T (1997) Human cytomegalovirus IE1 and IE2 proteins are mutagenic and mediate "hit-and-run" oncogenic transformation in cooperation with the adenovirus E1A proteins. Proc Natl Acad Sci USA 94: 3341-3345. 
• Szostek S, Klimek M, Zawilińska B, Ryś J, Kopeć J, Daszkiewicz E (2006) Detection of human papillomavirus in cervical cell specimens by hybrid capture and PCR with different primers. Acta Biochim Polon 53: 603-607. 
• Szostek S, Klimek M, Zawilińska B, Kosz-Vnenchak M (2008a) Genotype-specific human papillomavirus detection in cervical smears. Acta Biochim Polon 55: 687-692.  
• Szostek S, Klimek M, Zawilińska B, Kosz-Vnenchak M (2008b) Physical state of human papillomavirus type 16 in cervical cell DNA. Folia Biol (Kraków) 56: 269-271. 
• Venard V, Carret AS, Pascal N, Rihn B, Bordigoni P, Le Faou A (2000) A convenient semi-quantitative method for the diagnosis of Epstein-Barr virus reactivation. Arch Virol 145: 2211-2216. 
• Vo QN, Geradts J, Gulley ML, Boudreau DA, Bravo JC, Schneider BG (2002) Epstein-Barr virus in gastric adenocarcinomas: association with ethnicity and CDKN2A promoter methylation. J Clin Pathol 55: 669-75. 
• Yoshinouchi M, Hongo A, Nakamura K, Kodama J, Itoh S, Sakai H, Kudo T (1999) Analysis by multiplex PCR of the physical status of human papillomavirus type 16 DNA in cervical cancer. J Clin Microbiol 37: 3514-3517. 
• Zawilińska B, Bulek K, Kopeć J, Kosz-Vnenchak M (2006) In situ detection of DNA and mRNA of human cytomegalovirus to distinguish different forms of viral infection in leukocytes. Acta Biochim Polon 53: 457-461.  
• Zawilińska B, Kosińska A, Lenart M, Kopeć J, Piątkowska-Jakubas B, Skotnicki A, Kosz-Vnenchak M (2008) Detection of specific lytic and latent transcripts can help to predict the status of Epstein-Barr virus infection in transplant recipients with high virus load. Acta Biochim Polon 55: 693-699. 
• zur Hausen H (2000) Papillomaviruses causing cancer: evasion from host-cell control in early events in carcinogenesis. J Natl Cancer Inst 92: 690-698. 
• zur Hausen H (2002) Papillomaviruses and cancer: from basic studies to clinical application. Nat Rev Cancer 2: 342-350.