Antioxidative defense system in lupin roots exposed to increasing concentrations of lead

• Autorzy: Rucinska-Sobkowiak R , Pukacki P.M.
• Języki publikacji: EN

Abstrakty

EN

Yellow lupin (Lupinus luteus) cv. Juno seedlings exposed to increasing concentrations of Pb²⁺ (50-350 mg l⁻¹) were analysed in respect to its effect on the degradation of lipids, the content of antioxidants (ascorbate, α-tocopherol) and the activity of the ascorbate glutathione cycle enzymes (dehydroascorbate reductase DAR; EC 1.8.5.1 and glutathione reductase GR; EC 1.6.4.2). Lipid peroxidation, expressed as the content of TBArm (thiobarbituric acid reactive metabolites), increased only at 50 and 100 mgl⁻¹ Pb²⁺, whereas at higher lead concentrations it decreased as compared with the control. The level of free fatty acids was not significantly affected as a result of Pb²⁺ exposure, except for 300 mgl⁻¹ Pb²⁺. The content of α-tocopherol increased significantly at the range of concentrations between 150 and 300 mgl⁻¹ and at these concentrations lipid peroxidation was inhibited. Ascorbic acid (AA) and dehydroascorbic acid (DHA) content increased considerably in roots treated with 100 and 150 mgl⁻¹ Pb²⁺. In general the activity of DHAR and GR was stimulated by lead, however at higher Pb²⁺ concentrations (300 and 350 mgl⁻¹) GR revealed lower enzymatic activtty. Our results suggest that in lead-treated roots lipids might be protected against reactive oxygen species (ROS) by lipid-soluble molecules of α-tocopherol and this direct defense seems to be supported by AA as well as the enzymes: DHAR and GR.

Słowa kluczowe

EN

alpha-tocopherol; yellow lupin; plant physiology; Lupinus luteus; Juno cultivar; dehydroascorbate reductase; glutathione reductase; seedling; lupin; lead; root

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2006
• Tom: 28
• Numer: 4
• Opis fizyczny: p.357-364,fig.,fig.

Twórcy

autor

Rucinska-Sobkowiak R

• Adam Mickiewicz University, Al.Niepodleglosci 14, 61-713 Poznan, Poland

autor

Pukacki P.M.

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