A new automatized fluorometric assay for anti-Leishmania drug screening

• Autorzy: Le Pape P , Pagniez F , Abdala H
• Języki publikacji: EN

Abstrakty

EN

The therapeutic armentarium against leishmaniasis is very restricted, using mainly up to now drugs showing several side effects and resistance. Consequently, there is a real need to find new compounds as alternatives for the treatment of leishmaniasis. However, most of the classic antileishmanial primary screening assays are not suitable for measuring the cytotoxicity of large number of drug candidates because of the manipulations required. We have established a new assay that incorporate a fluorometric growth indicator based on the detection of a metabolic activity in culture medium after the chemical reduction of alamar Blue® by cells. This antileishmanial test was evaluated on amphotericin B, meglumine antimoniate, allopurinol, ketoconazole and edelfosine. The results reported show the advantages oj this fluorochrome on the tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT) for Leishmania cytotoxicity measurement. Moreover, this study is the first report of the use of alamar Blue® fluorogenic assay for activity assessment of potential antileishmanial drugs against promastigotes.

Słowa kluczowe

EN

antileishmanial drug; leishmaniasis; fluorometric assay; Leishmania mexicana; therapy; parasite proliferation

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2002
• Tom: 47
• Numer: 1
• Opis fizyczny: p.79-81,fig.

Twórcy

autor

Le Pape P

• Faculte de Pharmacie, Laboratoire de Parasitologie et Organisation Animale, UPRES EA 1155, 1 Rue Gaston Veil, 44035 Nantes Cedex, France
• Laboratoire de Parasitologie et de Mycologie Medicale, CHU Nantes, Quai Moncousu, 44000 Nantes, France

autor

Pagniez F

• Faculte de Pharmacie, Laboratoire de Parasitologie et Organisation Animale, UPRES EA 1155, 1 Rue Gaston Veil, 44035 Nantes Cedex, France

autor

Abdala H

• Faculte de Pharmacie, Laboratoire de Parasitologie et Organisation Animale, UPRES EA 1155, 1 Rue Gaston Veil, 44035 Nantes Cedex, France

Bibliografia

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