Multiplex-PCR assay for identification of Klebsiella pneumoniae isolates carrying the cps Loci for K1 and K2 capsule biosynthesis

• Autorzy: Gierczynski R , Jagielski M. , Rastawicki W. , Kaluzewski S.
• Języki publikacji: EN

Abstrakty

EN

Multiplex-PCR assay for identification of Klebsiella pneumoniae isolates carrying gene clusters for biosynthesis of capsular polysaccharide (CPS) types K1 and K2 was developed. Genes wzc and orflO of the cps cluster were applied as K1 and K2 specific markers respectively. The assay specificity was confirmed using 147 isolates of Klebsiella spp. including 77 K-antigen reference strains. The multiplex-PCR assay was found simple and cost-effective tool for identification of K. pneumoniae clinical isolates of K1 and K2 geno-serotypes.

Słowa kluczowe

EN

isolate; pathogen; wzc gene; K1 capsule; capsular polysaccharide; K2 capsule; biosynthesis; Klebsiella pneumoniae; genoserotyping; polymerase chain reaction; orf10 gene; identification

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2007
• Tom: 56
• Numer: 3
• Opis fizyczny: p.153-156,fig.,ref.

Twórcy

autor

Gierczynski R

• National Institute of Hygiene, Chocimska Street 24, 00-791 Warsaw, Poland

autor

Jagielski M.

autor

Rastawicki W.

autor

Kaluzewski S.

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