An easy procedure to transform the ratio of two polynomials of first degree into Michaelis-Menten-type equations. Application to the ordered Uni Bi enzyme mechanism

• Autorzy: Fontes R. , Ribeiro J.M. , Sillero A.
• Języki publikacji: EN

Abstrakty

EN

It is not always clear that some equations affected by complicated factors can, actually, be interpreted as a ratio of two polynomials of first degree and so that they can be, in general, represented by rectangular hyperbolas. In this paper we present an easy procedure to rearrange those equations into Michaelis-Menten-type equations and so to make the aspects of these rectangular hyperbolas more clear, particularly for researchers familiar with general biochemistry. As an example, the method is applied to transform the classical rate equation of the Cleland's Ordered Uni Bi enzyme mechanism.

Słowa kluczowe

EN

enzyme inhibition; enzyme equation; enzyme mechanism; enzyme kinetics

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2000
• Tom: 47
• Numer: 1
• Opis fizyczny: p.259-268,fig.

Twórcy

autor

Fontes R.

autor

Ribeiro J.M.

autor

Sillero A.

Bibliografia

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• 7. Hofstee, B.H.J. (1952) On the evaluation of the constants Vm and Km in enzyme reactions. Science 116, 329-331.