Effect of platelet-activating factor on motility, plasmalemma integrity, the process of capacitation and acrosome reaction of fresh and cryopreserved boar spermatozoa

• Autorzy: Kordan W. , Lecewicz M. , Tobolski J.
• Języki publikacji: EN

Abstrakty

EN

The aim of the present study was to establish the effects of platelet-activating factor (PAF) on selected movement parameters, plasmalemma integrity, capacitation process and acrosome reaction in cryopreserved boar spermatozoa. A positive effect of PAF addition to cryopreserved semen on sperm motility was demonstrated, particularly with the application of phospholipid concentration of 1 x 10-6M-1 x 10-5M. A moderate induction of plasmalemma damage of cryopreserved spermatozoa was observed when PAF was used at a low concentration (1 x 10-8M-1 x 10-7M). The rate at which PAF induced the process of capacitation was inversely proportional to its concentration in the sample (the highest for the concentration of 1 x 10-8M, and the lowest at 1 x 10-5M). In turn, the strongest induction of acrosome reaction of spermatozoa was observed in samples with the addition of PAF at a concentration of 1 x 10-7M. The results obtained suggest that the application of PAF supplement to post-thawed boar semen can be used as a laboratory test of the ability of spermatozoa to induce the acrosome reaction.

Słowa kluczowe

EN

boar; spermatozoon; motility; plasmalemma integrity; capacitation; acrosome reaction; fresh spermatozoon; cryopreserved spermatozoon; physiological function; platelet-activating factor

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2009
• Tom: 12
• Numer: 2
• Opis fizyczny: p.175-181,fig.,ref.

Twórcy

autor

Kordan W.

• University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-719 Olsztyn-Kortowo, Poland

autor

Lecewicz M.

autor

Tobolski J.

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