Poly[ADP-ribosyl]ation of proteins associated with nuclear matrix in rat testis

• Autorzy: Quesada P. , d'Erme M. , Atorino L. , Faraone-Mennella M.R. , Caiafa P. , Farina B.
• Języki publikacji: EN

Abstrakty

EN

We have previously demonstrated that a significant percentage of poly(ADPR) polymerase is present, as a tightly-bound form, at the third level of chromatin organisation defined by chromosomal loops and nuclear matrix. The present work is focused on the study of poly(ADP-ribosyl)ation of proteins present in these nuclear subfractions. It has been shown that, due to the action of poly(ADPR) polymerase, the ADP-ribose moiety of [14C]NAD is transferred to both loosely-bound and tightly-bound chromosomal proteins, which in consequence are modified by chain polymers of ADP-ribose of different lengths. Moreover, histone-like proteins seem to be ADP-ribosylated in chromosomal loops and nuclear matrix associated regions of DNA loops (MARS). A hypothesis can be put forward that the ADP-ribosylation system is functionally related to the nuclear processes, actively coordinated by the nuclear matrix.

Słowa kluczowe

EN

chromatin; matrix associated region; chromosomal loop; nuclear matrix; DNA; rat; chromosomal protein; spermatogenesis

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1995
• Tom: 42
• Numer: 2
• Opis fizyczny: p.153-160,fig.

Twórcy

autor

Quesada P.

• Universita Federico II, Naples

autor

d'Erme M.

autor

Atorino L.

autor

Faraone-Mennella M.R.

autor

Caiafa P.

autor

Farina B.

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