Oznaczanie sulfonamidow w miodach pszczelich metoda TLC

• Autorzy: Piekut J , Borawska M.H. , Rodziewicz L.

Warianty tytułu

EN

Determination of sulfonamides in bee honeys by the thin layer chromatography [TLC] method

• Języki publikacji: PL

Abstrakty

PL

Celem pracy było przeprowadzenie oznaczeń obecności sulfonamidów w dostępnych w handlu naturalnych miodach pszczelich z zastosowaniem chromatografii cienkowarstwowej (TLC) jako metody przesiewowej do wykrywania tych zanieczyszczeń.

EN

Methods of honey bee care against various diseases in Poland are based on usage of veterinary drugs, which can be found in bee products and cause their toxic effects on human organism. This research is focused on sulfa drugs - used as a veterinary drug against Bacillus larvae - etiological factor of American foulbrood disease. According to European Union law, any appearance of sulfa drugs is forbidden. Unfortunately Polisulfamid (mixture of three sulfonamides - sulfacetamide (SFA), sulfatiazole (SFT) and sulfadimethazine (SFM)) has used by polish beekeepers. Samples were prepared by solving 2.5 g of honey in 12.5 ml of acctic buffer (pH=5). The clean up of honey sample, mainly from sugar components, was performed on a solid phase extraction column. After a SPE percolation, clution of sulfonamides with acctonitrylc, evaporation in nitrogen stream and dilution in water was made. The prepared solution was placcd on Silica Gel for chromatography. After the thin layer chromatography is finished, the platelets arc revealed with fluoresceine and watched under UV lamp of wave length 254 nm. The method occurred to be sensitive enough to determine presence of sulfacetamide and sulfatiazole white sulfadimethazine contamination of honey bee honey in smallest quantities that previously have been determined using HPLC. It has been shown that the method is much easier to perform and cheaper for screening detection sulfonamides in natural honeys.

Słowa kluczowe

PL

konferencje; Bialystok konferencja; miod pszczeli; sulfonamidy; chromatografia cienkowarstwowa; oznaczanie

EN

conference; Bialystok conference; bee honey; sulphonamide; thin-layer chromatography; determination

• Wydawca: -
• Czasopismo: Bromatologia i Chemia Toksykologiczna
• Rocznik: 2005
• Tom: 38
• Numer: Supl.
• Opis fizyczny: s.395-398,rys.,bibliogr.

Twórcy

autor

Piekut J

• Akademia Medyczna, Bialystok

autor

Borawska M.H.

autor

Rodziewicz L.