Influence of the short-term road transport stress on blood parameters in cows

• Autorzy: Ali-Gholi R , Siamak A.-R. , Daryoush M.
• Języki publikacji: EN

Abstrakty

EN

Blood parameter changes have been investigated after short-term road transport stress in 18 crossbred dairy cows in northwest of Iran. Cows were transported in 4 groups of four cows and one group of two cows in five different days. Each group was transported by truck up to a 40 km round trip for an hour. Blood was taken in 5 ml amounts from the jugular vein from each cow before transport and then repeated bleeding at 1.5 hours intervals up to 7.5 hours after transportation. Blood samples were assessed for cortisol, (ELISA), glucose, calcium (Ca), phosphorus (IP), magnesium (Mg) (spectrophotometer method), sodium (Na), potassium (K) (flame Photometer), total protein (TPP), fibrinogen (Refractometer method), leucocytes and differential count. Blood cortisol, glucose and leucocytes count increased up to 340%, 48.6% and 23% within 1.5, 7.5 and 6 hours after transport, respectively. Haematocrit decreased up to 17% after 7.5 hours transport. The mean differences (Wilk.s Lambda) for these parameters before and after transport were significant (P < 0.05). The mean differences for Ca, IP, Mg and eosinophil count were also significant (P < 0.05). The highest changes were observed for glucose, hematocrit after 7.5 hours, Ca and the leucocytes count after 6 hours, cortisol after 1.5 hours and the lymphocyte count after 3 hours of transportation. There were significant (P < 0.05) relationships between cortisol&Mg (r = .0.54), cortisol&TPP (r = .0.55), cortisol&fibrinogen (r = .0.52), cortisol&neutrophil (r = .0.52) cortisol & lymphocyte count (r = 0.79) 3 hours after transport. It is concluded that short-term road transport stress increased blood cortisol, that changes in the blood parameters are mainly due to glucose, hematocrit, and leucocyte count and blood electrolytes. Although these changes were within the reference range for cattle, since these parameters are known as health parameters in a ruminant.s life they should be considered during the transportation.

Słowa kluczowe

EN

cow; blood parameter; transport stress; animal stress; dairy cow; Iran; blood sample; glucose; cortisol; hematocrit; calcium; health parameter; transportation

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2007
• Tom: 63
• Numer: 11
• Opis fizyczny: p.1311-1315,fig.,ref.

Twórcy

autor

Ali-Gholi R

• Urmia University, P.O.Box 1177, Urmia, Iran

autor

Siamak A.-R.

autor

Daryoush M.

Bibliografia

• 1.Arthington J. D., Eichert S. D., Kunkle W. E., Martin F. G.: Effect of transportation and commingling on the acute-phase protein response, growth and feed intake of newly weaned beef calves. J. Anim. Sci. 2003, 81, 1120-1125.
• 2.Berman A.: Tissue and external insulation estimates and their effects on prediction of energy requirements and of heat stress. J. Dairy Sci. 2004, 87, 1400-1412.
• 3.Binkhorst G. J., Heinrichs P. A., Ingh T. S., Hajer R., Nijkamp F. P.: The effects of stress on host defense system and on lung damage in calves experimentally infected with Pasteurella haemolytica. J. Vet. Med. 1990, A37, 525-536.
• 4.Blecha F., Boyles S. L., Riley J. G.: Shipping suppresses lymphocyte blastogenic responses in Angus and Brahman X Angus feeder calves. J. Anim. Sci. 1984, 59, 576-583.
• 5.Brealey J. C., Dobson H., Jones R. S.: Investigations into the effect of two sedatives on the stress response in cattle. J. Vet. Pharmacol. Therp. 1990, 13, 367-377.
• 6.Dobson H.: Effect of transport stress on Luteinizing hormone released by GnRH in dairy cows. Acta. Endocrinologica 1987, 115, 63-66.
• 7.Gentry P. A., Liptrap R. M., Tremblay R. R., Lichen L., Ross M. L.: Adrenocorticotrophic hormone fails to alter plasma fibrinogen and fibronectin values in calves but does so in rabbits. Vet. Res. Commun. 1992, 16, 253-264.
• 8.Hickey M. C., Dreanan M., Early B.: The effect of abrupt weaning of suckling calves on the plasma concentrations of cortisol, catacholamines, leukocytes, acute-phase proteins and in vitro interferon-gamma production. J. Anim. Sci. 2003, 81, 2847-2855.
• 9.Kent J. E., Ewbank R.: The effect of road transportation on the blood constituents and behavior of calves. 1. Six months old. Br. Vet. J. 1983, 139, 228-235.
• 10.Knowles T. G., Brown S. N., Warriss P. D., Philips A. J., Dolan S. K., Hunt P., Ford J. E., Edwards J. E., Watkines P. E.: Effects on sheep of transport by road for up to 24 hours. Vet. Rec. 1995, 136, 431-438.
• 11.Kumar B. R., Muralidharan M. R., Ramesh V., Arunachalam S., Sivakumar T.: Effect of Transport stress on blood profile in sheep. Indian Vet. J. 2003, 80, 511-514.
• 12.Maraherens M., Von-Richthofen I., Schmeiduch S., Hartung J.: Special problems of long distance road transports of cattle. Dtsch. Tierärztl. Wschr. 2003, 110, 120-125.
• 13.McDougall S., Anniss F. M., Cullum A. A.: Effect of transport stress on somatic cell counts in dairy goats. Proceeding of the NZ Society of Anim. Prod. 2002, 62, 16-18.
• 14.Mitchell G., Hattingh J., Ganhao M.: Stress in cattle assessed after handling, after transport and after slaughter. Vet. Rec. 1988, 123, 201-205.
• 15.Nazifi S., Rezakani A., Mohammadi G. R., Ojaghi A.: Effect of transportation stress on the haematologic parameters in calves. J. Faculty of Vet. Med., University of Tehran 2003, 57, 71-76.
• 16.Orihuela A., Sanchez-mejorada H., Toledo M.: Effect of short transport during dioestrus and proestrus on cortisol levels and estrous behavior of sheep. J. Agri. Sci. 2002, 138, 93-96.
• 17.Phillips W. A.: The effect of assembly and transit stressors on plasma fibrinogen concentration of beef calves. J. comp. Med. 1984, 48, 35-41.
• 18.Ramin A. G.: Physiological response tests and blood profiles in replacement dairy heifers and their relationship to growth rates and health parameters. Thesis, The University of Queensland 1995, pp. 23-33.
• 19.Sanhouri A. A., Jones R. S., Dobson H.: Effects of Xylazine on the stress response to transport in male goats. J. Br. Vet. 1992, 148, 119-128.
• 20.Sartorelli P., Dominoni S., Agens F.: Influence of duration of simulated transport on plasma stress markers in the calf. Zntbl. Vet. Med. 1992, 39, 401-403.
• 21.Schaefer A. L., Jones R. C., Dobson H.: Pentobarbitone inhibits the stress response to transport in male goats. J. Br. Vet. 1991, 147, 42-48.
• 22.Simone M., Holt A. C., John B., Gaughan A. D., Terry L., Madder B.: Feeding Strategies for grain fed cattle in a hot environment. Australia. J. Agri. 2004, 55, 719-725.
• 23.Steinhardt M.: Reactions of young cattle from a suckler herd to short transport by road repeated investigations before and after permanent separation of young cattle from their dams. Plasma, cortisol, biochemical, hematological variables, minerals and heart rate. Dtsch. Tierärztl. Wschr. 2002, 109, 239-245.
• 24.Steinhardt M., Thielscher H. H.: Transport stress in monozygotic twin calves. Tierärztliche Umschau 2003, 58, 645-650.
• 25.Steinhardt M., Thielscher H. H., Rath D.: Reactions of non pregnant and of cattle at different stages of pregnancy from the Holstein and Friesian breed (HF) and from the old type German black and white breed exposed to transport stress. Dtsch. Tierärztl. Wschr. 1997, 104, 505-512.
• 26.Tarrant P. V., Kenny F. J., Harnngton D.: The effect of stocking density during 4 hour transport to slaughter on behavior blood constituents and carcass bruising in Friesian stress. Meat. Sci. 1988, 24, 209-222.
• 27.Villarroel M., Maria G., Sanudo C., Garcia S., Chacon G., Senbet G.: Effect of commercial transport in Spain on cattle welfare and meat quality. Dtsch. Tierärztl. Wschr. 2003, 110, 105-107.
• 28.West J. W.: Effects of heat stress on production in dairy cattle. Dtsch. Tierärztl. Wschr. 2003, 110, 100-104.
• 29.Wikner I., Gebresenbet G., Nilsson C.: Assessment of air quality in a commercial cattle transport vehicle in Swedish summer and winter conditions. J. Dairy Sci. 2003, 84, 2314-2320.
• 30.Zavy M. T., Juniewicz P. E., Phillips W. A., Vontugeln D. L.: Effect of initial restraint, weaning and transport stress on baseline and ACTH stimulated cortisol responses in beef calves of different genotypes. J. Vet. Rec. 1992, 53, 551-557.
• 31.Zhou C., Chen H. Q., Reeves R., Agarwal N., Cammarata P. R.: Protective mechanism against water stress. Invest. Ophtalmol. Vis. Sci. 1994, 35, 4118-4125