Analysis of microsatellite instability and loss of heterozygosity in breast cancer with the use of a well characterized multiplex system

• Autorzy: Powierska-Czarny J. , Miscicka-Sliwka D. , Czarny J. , Grzybowski T. , Wozniak M. , Drewa G. , Czechowicz W. , Sir J.
• Języki publikacji: EN

Abstrakty

EN

Analysis of microsatellite instability (MI) and loss of heterozygosity (LOH) is rec­ommended for screening patients with sporadic and hereditary malignancies. This study shows an application of a fluorescent hexaplex PCR system for microsatellite typing on A.L.F. DNA Sequencer (Pharmacia Biotech). This technique detects changes in microsatellites providing a time-efficient, reliable and accurate method for MI and LOH analyses. The Fragment Manager software was used for automated size calculation and quantitation of DNA fragments, enabling rapid and precise mea­surement of allelic ratios. We examined 70 breast cancer and 70 control DNA speci­mens, classified all the patterns of microsatellite alterations, and set up MI and LOH assessment criteria for the automated multiplex fluorescent method.

Słowa kluczowe

EN

microsatellite instability; short tandem repeat; multiplex DNA typing; heterozygosity; breast cancer

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 4
• Opis fizyczny: p.1195-1203,fig.,ref.

Twórcy

autor

Powierska-Czarny J.

• The Ludwik Rydygier Medical University of Bydgoszcz, Powstancow Wielkopolskich 44-39, 85-090 Bydgoszcz, Poland

autor

Miscicka-Sliwka D.

autor

Czarny J.

autor

Grzybowski T.

autor

Wozniak M.

autor

Drewa G.

autor

Czechowicz W.

autor

Sir J.

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