Recombinant ferritin protects mice against challenge with Echinococcus granulosus?

• Autorzy: Wang Y , Li Z. , Li Z. , Bo Y. , Zhao W.
• Języki publikacji: EN

Abstrakty

EN

Ferritin is well known as the main intracellular iron storage protein in both prokaryotes and eukaryotes, keeping it in a soluble and non-toxic form, though the role of ferritin as a vaccine candidate in echinococcosis has not yet been delineated. Through our study, ferritin was cloned from Echinococcus granulosus and expressed in Escherichia coli. The recombinant E. granulosus ferritin (rEgferritin) has a molecular weight of 19 kDa and could be recognized by anti-mice serum in Western blotting. The specific antibody was detected by enzyme-linked immunosorbent assay. Mice vaccinated with rEgferritin and challenged intraperitoneally with E. granulosus protoscoleces revealed significant protective efficacy up to 85.6%, compared with the control group. Thus, rEgferritin could be a promising candidate as an effective vaccine to prevent the infection of echinococcosis.

Słowa kluczowe

EN

ferritin; intracellular iron; storage protein; prokaryote; eukaryote; non-toxic form; vaccine; Echinococcus granulosus; echinococcosis; hydatid disease; mice; mouse

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2009
• Tom: 54
• Numer: 4
• Opis fizyczny: p.335-340,fig.,ref.

Twórcy

autor

Wang Y

• Center of Scientific Technology of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China

autor

Li Z.

autor

Li Z.

autor

Bo Y.

autor

Zhao W.

Bibliografia

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