Species-specific chloroplast DNA polymorphism in the trnV-rbcL region in Pinus sylvestris and P. mugo

• Autorzy: Wachowiak W , Baczkiewicz A. , Celinski K. , Prus-Glowacki W.
• Języki publikacji: EN

Abstrakty

EN

Four cpDNA regions were analyzed with the use of PCR-RFLP technique and nucleotide sequences of two mtDNA regions were characterized in order to find P. sylvestris and P. mugo species specific markers useful for studies of the species hybridization. The difference in the restriction fragment patterns of trnV-rbcL region after digestion with MvaI endonuclease was detected. The analyses of the species representatives from various geographic regions revealed that the observed polymorphism is species specific. No differences have been disclosed in the analyzed trnS-trnT, trnK1-trnK2, trnC-trnD cpDNA regions. The P. sylvestris and P.mugo mtDNA sequences of orf25 and coxI regions proved to be identical.

Słowa kluczowe

EN

Scotch pine; Pinus sylvestris; Pinus mugo; dwarf pine; hybridization; DNA marker; mtDNA; trnV-rbcL region; chloroplast; DNA polymorphism

• Wydawca: -
• Czasopismo: Dendrobiology
• Rocznik: 2004
• Tom: 51
• Opis fizyczny: p.67-72,fig.,ref.

Twórcy

autor

Wachowiak W

• Polish Academy of Sciences, Institute of Dendrology, Parkowa 5, 62-035 Kornik, Poland

autor

Baczkiewicz A.

autor

Celinski K.

autor

Prus-Glowacki W.

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