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2006 | 62 | 09 |

Tytuł artykułu

Mieszane zakazenia konskimi herpeswirusami typu 1 i 2 [EHV-1 i EHV-2]

Warianty tytułu

EN
Mixed infections of equine herpes virus types 1 and 2 [EHV-1 and EHV-2]

Języki publikacji

PL

Abstrakty

EN
110 blood samples from clinically healthy mares of English breed, half-blood and the Wielkopolska race were tested for the presence of EHV-1 and EHV-2 using nested PCR. 15 samples were EHV-1-positive, 24 samples were EHV-2-positive, whereas only 4 samples were both EHV-1 and EHV-2 positive. The virus was isolated from PBLs in equine dermal cell cultures by co-cultivation or by culture inoculation with cell lysates derived from PBLs. A total of 14 strains were isolated from EHV-2 and EHV-1/EHV-2-positive samples. However, all of them were identified by nPCR as being type 2. Since no EHV-1 was isolated, even from dually infected leukocytes, it was concluded that the presence of EHV-2 does not stimulate in-vitro isolation of EHV-1 from infected leukocytes. It is tempting to speculate that such stimulatory effects in-vivo may involve EHV-2-induced immunosupression. Despite any possible mechanism of EHV-1 stimulation, it seems that EHV-2 does not play a significant role in the epidemiology of EHV-1-caused miscarriages in horses since mixed infections are rather rare.

Wydawca

-

Rocznik

Tom

62

Numer

09

Opis fizyczny

s.1071-1072,tab.,bibliogr.

Twórcy

autor
  • Szkola Glowna Gospodarstwa Wiejskiego, ul.Ciszewskiego 8, 02-786 Warszawa
autor
autor

Bibliografia

  • 1.Allen G. P., Bryans J. T.: Molecular epizootiology, pathogenesis, and prophylaxis of equine herpesvirus-1 infections. Prog. Vet. Microb. Imm. 1986, 2, 78-144.
  • 2.Allen G. P., Yeargan M. R., Bryans J. T.: Alterations in the equine herpesvirus 1 genome after in vitro and in vivo virus passage. Infect. Immun. 1983, 40, 436-439.
  • 3.Banbura M. W., Chmielewska A., Tucholska A., Malicki K.: Występowanie wirusa zakaźnego ronienia klaczy w leukocytach krwi obwodowej koni. Medycyna Wet. 2000, 56, 521-523.
  • 4.Bańbura M. W.: Produktywne i latentne zakażenia końskim herpeswirusem typu 1 (EHV-1). Wyd. SGGW 2003.
  • 5.Belak S., Palfi V., Tuboly S., Bartha L.: Passive immunization of foals to prevent respiratory disease caused by equine herpesvirus type 2. Zbl. Vet. Med. 1980, 27, 826-830.
  • 6.Blakeslee J. R., Olsen R. G., McAllister E. S., Fassbender J., Dennis R.: Evidence of respiratory tract infection induced by equine herpesvirus, type 2, in the horse. Can. J. Mirobiol. 1975, 21, 1940-1946.
  • 7.Borchers K., Slater J.: A nested PCR for the detection and differentiation of EHV-1 and EHV-4. J. Virol. Meth. 1993, 45, 331-336.
  • 8.Borchers K., Wolfinger U., Golz M., Broll H., Ludwig H.: Distribution and relevance of equine herpesvirus type 2 (EHV-2) infections. Arch. Virol. 1997, 142, 917-928.
  • 9.Borchers K., Wolfinger U., Ludwig H., Thein P., Baxi S., Field H. J., Slater J. D.: Virological and molecular biological investigation into equine herpes virus type 2 (EHV-2) experimental infections. Virus Res. 1998, 55, 101-106.
  • 10.Browning G. F., Studdert M. J.: Equine herpesvirus 2 (equine cytomegalovirus). Vet. Bull. 1988, 58, 775-790.
  • 11.Collinson P. N., O'Rielly J. L., Ficorilli N., Studdert M. J.: Isolation of equine herpesvirus type 2 (equine gammaherpesvirus 2) from foals with keratoconjunctivitis. J. Am. Vet. Med. Assoc. 1994, 205, 329-331.
  • 12.Dzieciątkowski T.: Wpływ końskiego herpeswirusa typu 2 (EHV-2) na produktywne zakażenie wirusem zakaźnego ronienia klaczy (EHV-1) w warunkach in vitro. Praca doktorska, Wydz. Med. Wet. SGGW, Warszawa 2004.
  • 13.Harden T. J., Bagust T. J., Pascoe R. R., Spradbrow P. B.: Studies on equine herpesviruses. 5. Isolation and characterization of slowly cytopathic equine herpesviruses in Queensland. Aust. Vet. J. 1974, 50, 483-488.
  • 14.Kemeny L., Pearson J. E.: Isolation of herpesvirus from equine leukocytes: comparison with equine rhinopneumonitis virus. Can. J. Comp. Med. 1970, 34, 59-65.
  • 15.Nordegrahn A., Rusvai M., Merza M., Ekström J., Morein B., Belak S.: Equine herpesvirus type 2 (EHV-2) as a predisposing factor for Rhodococcus equi pneumonia in foals: prevention of the bifactoral disease with EHV-2 immunostimulating complexes. Vet. Mocrobiol. 1996, 51, 55-68.
  • 16.Palfi V., Belak S., Molnar T.: Isolation of herpesvirus type 2 from foals showing respiratory symptoms. Zbl. Vet. Med. 1978, 25, 165-167.
  • 17.Roeder P. L., Scott G. R.: The prevalence of of equid herpes virus 2 infections. Vet. Rec. 1975, 96, 404-405.
  • 18.Ruszczyk A., Chmielewska A., Tucholska A., Bańbura M. W.: Izolacja i identyfikacja herpeswirusa koni typu 2 (EHV-2). Medycyna Wet. 2001, 57, 603-606.
  • 19.Strauss W. M.: Preparation of genomic DNA from Mammalian tissue, [w:] Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. (red.): Current Protocols in Molecular Biology. Greene Publishing Associates and Wiley Interscience, New York 1992, 2.2.1.-2.2.3.
  • 20.Van Maanen C.: Equine herpesvirus 1 and 4 infections: an update. Vet. Quart. 2002, 24, 57-78.
  • 21.Welch H. M., Bridges C. G., Lyon A. M., Griffiths L., Edington N.: Latent equid herpesvirus 1 and 4: detection using the polymerase chain reaction and cocultivation from lymphoid tissues. J. Gen Virol. 1992, 73, 261-268.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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